Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS18775 and CA265_RS18780 are separated by 326 nucleotides CA265_RS18780 and CA265_RS18785 are separated by 58 nucleotides CA265_RS18785 and CA265_RS18790 are separated by 68 nucleotides
CA265_RS18775: CA265_RS18775 - hypothetical protein, at 4,672,309 to 4,673,040
_RS18775
CA265_RS18780: CA265_RS18780 - YicC family protein, at 4,673,367 to 4,674,230
_RS18780
CA265_RS18785: CA265_RS18785 - guanylate kinase, at 4,674,289 to 4,674,855
_RS18785
CA265_RS18790: CA265_RS18790 - four helix bundle protein, at 4,674,924 to 4,675,292
_RS18790
Position (kb)
4673
4674
4675 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4672.393 kb on - strand, within CA265_RS18775 at 4672.401 kb on + strand, within CA265_RS18775 at 4672.401 kb on + strand, within CA265_RS18775 at 4672.401 kb on + strand, within CA265_RS18775 at 4672.537 kb on + strand, within CA265_RS18775 at 4672.539 kb on + strand, within CA265_RS18775 at 4672.547 kb on + strand, within CA265_RS18775 at 4672.547 kb on + strand, within CA265_RS18775 at 4672.547 kb on + strand, within CA265_RS18775 at 4672.814 kb on - strand, within CA265_RS18775 at 4672.814 kb on - strand, within CA265_RS18775 at 4672.814 kb on - strand, within CA265_RS18775 at 4672.859 kb on + strand, within CA265_RS18775 at 4672.940 kb on + strand, within CA265_RS18775 at 4673.010 kb on + strand at 4673.011 kb on - strand at 4673.047 kb on - strand at 4673.064 kb on + strand at 4673.152 kb on - strand at 4673.319 kb on + strand at 4673.319 kb on + strand at 4673.320 kb on - strand at 4673.383 kb on + strand at 4673.605 kb on + strand, within CA265_RS18780 at 4673.619 kb on + strand, within CA265_RS18780 at 4673.639 kb on + strand, within CA265_RS18780 at 4673.709 kb on + strand, within CA265_RS18780 at 4673.878 kb on + strand, within CA265_RS18780 at 4673.988 kb on + strand, within CA265_RS18780 at 4674.001 kb on + strand, within CA265_RS18780 at 4674.008 kb on + strand, within CA265_RS18780 at 4674.128 kb on + strand, within CA265_RS18780 at 4674.175 kb on + strand at 4674.179 kb on + strand at 4674.854 kb on - strand at 4674.889 kb on + strand at 4674.892 kb on - strand at 4674.892 kb on - strand at 4674.911 kb on + strand at 4674.914 kb on - strand at 4674.914 kb on - strand at 4674.944 kb on - strand at 4675.095 kb on - strand, within CA265_RS18790 at 4675.098 kb on + strand, within CA265_RS18790 at 4675.100 kb on + strand, within CA265_RS18790 at 4675.166 kb on - strand, within CA265_RS18790 at 4675.184 kb on + strand, within CA265_RS18790 at 4675.188 kb on + strand, within CA265_RS18790 at 4675.188 kb on + strand, within CA265_RS18790 at 4675.189 kb on - strand, within CA265_RS18790 at 4675.216 kb on + strand, within CA265_RS18790 at 4675.223 kb on - strand, within CA265_RS18790
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 4,672,393 - CA265_RS18775 0.11 +1.7 4,672,401 + CA265_RS18775 0.13 +1.3 4,672,401 + CA265_RS18775 0.13 +1.2 4,672,401 + CA265_RS18775 0.13 -0.0 4,672,537 + CA265_RS18775 0.31 +0.6 4,672,539 + CA265_RS18775 0.31 +1.2 4,672,547 + CA265_RS18775 0.33 +0.9 4,672,547 + CA265_RS18775 0.33 -0.0 4,672,547 + CA265_RS18775 0.33 +1.2 4,672,814 - CA265_RS18775 0.69 -0.6 4,672,814 - CA265_RS18775 0.69 -0.2 4,672,814 - CA265_RS18775 0.69 -1.4 4,672,859 + CA265_RS18775 0.75 -0.0 4,672,940 + CA265_RS18775 0.86 -0.0 4,673,010 + -0.7 4,673,011 - -0.7 4,673,047 - +0.1 4,673,064 + -2.2 4,673,152 - +0.2 4,673,319 + +0.4 4,673,319 + +1.3 4,673,320 - +0.3 4,673,383 + +1.8 4,673,605 + CA265_RS18780 0.28 +1.1 4,673,619 + CA265_RS18780 0.29 +1.2 4,673,639 + CA265_RS18780 0.31 +1.4 4,673,709 + CA265_RS18780 0.40 +1.8 4,673,878 + CA265_RS18780 0.59 +1.3 4,673,988 + CA265_RS18780 0.72 +2.7 4,674,001 + CA265_RS18780 0.73 +1.2 4,674,008 + CA265_RS18780 0.74 +1.1 4,674,128 + CA265_RS18780 0.88 +0.8 4,674,175 + +2.0 4,674,179 + +1.0 4,674,854 - -1.2 4,674,889 + +1.0 4,674,892 - -2.0 4,674,892 - -0.2 4,674,911 + -0.0 4,674,914 - +0.5 4,674,914 - +2.2 4,674,944 - -0.7 4,675,095 - CA265_RS18790 0.46 -1.2 4,675,098 + CA265_RS18790 0.47 -1.4 4,675,100 + CA265_RS18790 0.48 -0.8 4,675,166 - CA265_RS18790 0.66 -0.2 4,675,184 + CA265_RS18790 0.70 +0.5 4,675,188 + CA265_RS18790 0.72 -0.7 4,675,188 + CA265_RS18790 0.72 -0.6 4,675,189 - CA265_RS18790 0.72 -1.8 4,675,216 + CA265_RS18790 0.79 -0.1 4,675,223 - CA265_RS18790 0.81 +0.3
Or see this region's nucleotide sequence