Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS18730 and CA265_RS18735 are separated by 352 nucleotides CA265_RS18735 and CA265_RS18740 are separated by 86 nucleotides CA265_RS18740 and CA265_RS18745 are separated by 150 nucleotides CA265_RS18745 and CA265_RS18750 are separated by 158 nucleotides CA265_RS18750 and CA265_RS18755 are separated by 21 nucleotides
CA265_RS18730: CA265_RS18730 - hypothetical protein, at 4,663,756 to 4,665,483
_RS18730
CA265_RS18735: CA265_RS18735 - tRNA-Ser, at 4,665,836 to 4,665,923
_RS18735
CA265_RS18740: CA265_RS18740 - pyruvate kinase, at 4,666,010 to 4,667,440
_RS18740
CA265_RS18745: CA265_RS18745 - hypothetical protein, at 4,667,591 to 4,668,010
_RS18745
CA265_RS18750: CA265_RS18750 - acyl carrier protein, at 4,668,169 to 4,668,405
_RS18750
CA265_RS18755: CA265_RS18755 - beta-ketoacyl-[acyl-carrier-protein] synthase II, at 4,668,427 to 4,669,680
_RS18755
Position (kb)
4666
4667
4668 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4665.029 kb on - strand, within CA265_RS18730 at 4665.029 kb on - strand, within CA265_RS18730 at 4665.195 kb on + strand, within CA265_RS18730 at 4665.195 kb on + strand, within CA265_RS18730 at 4665.196 kb on - strand, within CA265_RS18730 at 4665.216 kb on + strand, within CA265_RS18730 at 4665.228 kb on + strand, within CA265_RS18730 at 4665.250 kb on - strand, within CA265_RS18730 at 4665.282 kb on + strand, within CA265_RS18730 at 4665.282 kb on + strand, within CA265_RS18730 at 4665.282 kb on + strand, within CA265_RS18730 at 4665.282 kb on + strand, within CA265_RS18730 at 4665.358 kb on - strand at 4665.358 kb on - strand at 4665.397 kb on + strand at 4665.428 kb on - strand at 4665.467 kb on - strand at 4665.520 kb on - strand at 4665.553 kb on - strand at 4665.604 kb on - strand at 4665.604 kb on - strand at 4665.614 kb on + strand at 4665.615 kb on - strand at 4665.636 kb on + strand at 4665.760 kb on + strand at 4665.794 kb on + strand at 4665.794 kb on + strand at 4665.825 kb on + strand at 4665.877 kb on - strand, within CA265_RS18735 at 4666.001 kb on + strand at 4666.293 kb on - strand, within CA265_RS18740 at 4667.480 kb on - strand at 4667.585 kb on + strand at 4667.786 kb on + strand, within CA265_RS18745 at 4667.790 kb on + strand, within CA265_RS18745 at 4667.934 kb on + strand, within CA265_RS18745 at 4668.055 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 4,665,029 - CA265_RS18730 0.74 +0.2 4,665,029 - CA265_RS18730 0.74 +1.5 4,665,195 + CA265_RS18730 0.83 -0.2 4,665,195 + CA265_RS18730 0.83 +0.3 4,665,196 - CA265_RS18730 0.83 +0.3 4,665,216 + CA265_RS18730 0.84 +1.7 4,665,228 + CA265_RS18730 0.85 +0.4 4,665,250 - CA265_RS18730 0.86 +0.5 4,665,282 + CA265_RS18730 0.88 +0.4 4,665,282 + CA265_RS18730 0.88 +0.4 4,665,282 + CA265_RS18730 0.88 +0.9 4,665,282 + CA265_RS18730 0.88 -0.6 4,665,358 - -1.4 4,665,358 - +0.5 4,665,397 + +0.4 4,665,428 - +0.5 4,665,467 - +0.4 4,665,520 - +1.8 4,665,553 - -0.2 4,665,604 - -0.8 4,665,604 - -0.1 4,665,614 + +1.0 4,665,615 - +1.0 4,665,636 + +0.5 4,665,760 + +0.4 4,665,794 + -0.7 4,665,794 + -0.2 4,665,825 + +0.4 4,665,877 - CA265_RS18735 0.47 -0.2 4,666,001 + +0.6 4,666,293 - CA265_RS18740 0.20 -2.6 4,667,480 - +0.5 4,667,585 + -2.0 4,667,786 + CA265_RS18745 0.46 -1.0 4,667,790 + CA265_RS18745 0.47 -2.8 4,667,934 + CA265_RS18745 0.82 -0.6 4,668,055 - -1.4
Or see this region's nucleotide sequence