Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS17965 and CA265_RS17970 are separated by 180 nucleotides CA265_RS17970 and CA265_RS17975 are separated by 156 nucleotides CA265_RS17975 and CA265_RS17980 are separated by 170 nucleotides
CA265_RS17965: CA265_RS17965 - N-acyl-L-amino acid amidohydrolase, at 4,490,162 to 4,491,466
_RS17965
CA265_RS17970: CA265_RS17970 - hypothetical protein, at 4,491,647 to 4,492,156
_RS17970
CA265_RS17975: CA265_RS17975 - glucose-6-phosphate isomerase, at 4,492,313 to 4,493,959
_RS17975
CA265_RS17980: CA265_RS17980 - peptidase M16, at 4,494,130 to 4,495,443
_RS17980
Position (kb)
4492
4493
4494 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4491.398 kb on + strand at 4491.475 kb on + strand at 4491.476 kb on - strand at 4491.476 kb on - strand at 4491.479 kb on + strand at 4491.494 kb on - strand at 4491.542 kb on + strand at 4491.542 kb on + strand at 4491.543 kb on - strand at 4491.563 kb on + strand at 4491.582 kb on - strand at 4491.676 kb on + strand at 4491.677 kb on - strand at 4491.727 kb on + strand at 4491.728 kb on - strand, within CA265_RS17970 at 4491.748 kb on + strand, within CA265_RS17970 at 4491.848 kb on + strand, within CA265_RS17970 at 4491.863 kb on - strand, within CA265_RS17970 at 4491.865 kb on + strand, within CA265_RS17970 at 4491.865 kb on + strand, within CA265_RS17970 at 4491.915 kb on - strand, within CA265_RS17970 at 4491.915 kb on - strand, within CA265_RS17970 at 4491.915 kb on - strand, within CA265_RS17970 at 4491.959 kb on - strand, within CA265_RS17970 at 4491.973 kb on + strand, within CA265_RS17970 at 4491.984 kb on - strand, within CA265_RS17970 at 4491.984 kb on - strand, within CA265_RS17970 at 4492.006 kb on + strand, within CA265_RS17970 at 4492.016 kb on + strand, within CA265_RS17970 at 4492.091 kb on - strand, within CA265_RS17970 at 4492.097 kb on - strand, within CA265_RS17970 at 4492.103 kb on + strand, within CA265_RS17970 at 4492.104 kb on - strand, within CA265_RS17970 at 4492.118 kb on + strand at 4492.156 kb on + strand at 4492.156 kb on + strand at 4492.157 kb on - strand at 4492.162 kb on - strand at 4492.191 kb on + strand at 4492.205 kb on + strand at 4492.259 kb on + strand at 4493.962 kb on + strand at 4493.982 kb on + strand at 4493.986 kb on + strand at 4494.066 kb on - strand at 4494.243 kb on - strand at 4494.282 kb on - strand, within CA265_RS17980 at 4494.286 kb on + strand, within CA265_RS17980 at 4494.287 kb on - strand, within CA265_RS17980 at 4494.287 kb on - strand, within CA265_RS17980 at 4494.287 kb on - strand, within CA265_RS17980 at 4494.321 kb on + strand, within CA265_RS17980 at 4494.399 kb on - strand, within CA265_RS17980 at 4494.409 kb on - strand, within CA265_RS17980 at 4494.494 kb on + strand, within CA265_RS17980 at 4494.527 kb on + strand, within CA265_RS17980 at 4494.564 kb on - strand, within CA265_RS17980 at 4494.566 kb on + strand, within CA265_RS17980 at 4494.590 kb on + strand, within CA265_RS17980 at 4494.689 kb on + strand, within CA265_RS17980 at 4494.689 kb on + strand, within CA265_RS17980 at 4494.690 kb on - strand, within CA265_RS17980 at 4494.736 kb on + strand, within CA265_RS17980 at 4494.737 kb on - strand, within CA265_RS17980 at 4494.737 kb on - strand, within CA265_RS17980 at 4494.777 kb on - strand, within CA265_RS17980 at 4494.780 kb on + strand, within CA265_RS17980 at 4494.782 kb on + strand, within CA265_RS17980 at 4494.783 kb on - strand, within CA265_RS17980 at 4494.824 kb on + strand, within CA265_RS17980 at 4494.828 kb on + strand, within CA265_RS17980 at 4494.845 kb on + strand, within CA265_RS17980 at 4494.845 kb on + strand, within CA265_RS17980 at 4494.846 kb on - strand, within CA265_RS17980 at 4494.907 kb on - strand, within CA265_RS17980 at 4494.907 kb on - strand, within CA265_RS17980
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 4,491,398 + +0.9 4,491,475 + +1.4 4,491,476 - -0.1 4,491,476 - -0.8 4,491,479 + +0.1 4,491,494 - +0.9 4,491,542 + +0.2 4,491,542 + +1.1 4,491,543 - -0.6 4,491,563 + -0.3 4,491,582 - +0.8 4,491,676 + +0.8 4,491,677 - -1.2 4,491,727 + -0.2 4,491,728 - CA265_RS17970 0.16 +0.9 4,491,748 + CA265_RS17970 0.20 -0.3 4,491,848 + CA265_RS17970 0.39 -0.1 4,491,863 - CA265_RS17970 0.42 +0.1 4,491,865 + CA265_RS17970 0.43 +0.8 4,491,865 + CA265_RS17970 0.43 +0.3 4,491,915 - CA265_RS17970 0.53 +1.8 4,491,915 - CA265_RS17970 0.53 +1.4 4,491,915 - CA265_RS17970 0.53 +0.4 4,491,959 - CA265_RS17970 0.61 -0.1 4,491,973 + CA265_RS17970 0.64 +0.9 4,491,984 - CA265_RS17970 0.66 -0.4 4,491,984 - CA265_RS17970 0.66 -0.5 4,492,006 + CA265_RS17970 0.70 +1.5 4,492,016 + CA265_RS17970 0.72 +0.1 4,492,091 - CA265_RS17970 0.87 -0.3 4,492,097 - CA265_RS17970 0.88 -1.0 4,492,103 + CA265_RS17970 0.89 +1.1 4,492,104 - CA265_RS17970 0.90 -0.8 4,492,118 + +1.0 4,492,156 + +0.9 4,492,156 + -1.0 4,492,157 - +0.3 4,492,162 - -0.3 4,492,191 + +1.9 4,492,205 + -1.2 4,492,259 + +0.6 4,493,962 + -0.7 4,493,982 + -0.0 4,493,986 + -3.6 4,494,066 - +0.7 4,494,243 - +0.2 4,494,282 - CA265_RS17980 0.12 +1.0 4,494,286 + CA265_RS17980 0.12 +1.4 4,494,287 - CA265_RS17980 0.12 -0.0 4,494,287 - CA265_RS17980 0.12 +0.5 4,494,287 - CA265_RS17980 0.12 +0.2 4,494,321 + CA265_RS17980 0.15 +0.2 4,494,399 - CA265_RS17980 0.20 +0.4 4,494,409 - CA265_RS17980 0.21 -0.3 4,494,494 + CA265_RS17980 0.28 +0.3 4,494,527 + CA265_RS17980 0.30 +1.6 4,494,564 - CA265_RS17980 0.33 +0.3 4,494,566 + CA265_RS17980 0.33 +0.0 4,494,590 + CA265_RS17980 0.35 +1.0 4,494,689 + CA265_RS17980 0.43 +0.3 4,494,689 + CA265_RS17980 0.43 +0.2 4,494,690 - CA265_RS17980 0.43 +0.1 4,494,736 + CA265_RS17980 0.46 +1.1 4,494,737 - CA265_RS17980 0.46 -0.2 4,494,737 - CA265_RS17980 0.46 +0.4 4,494,777 - CA265_RS17980 0.49 +0.4 4,494,780 + CA265_RS17980 0.49 -0.3 4,494,782 + CA265_RS17980 0.50 +0.4 4,494,783 - CA265_RS17980 0.50 -0.7 4,494,824 + CA265_RS17980 0.53 +0.8 4,494,828 + CA265_RS17980 0.53 +0.4 4,494,845 + CA265_RS17980 0.54 -3.1 4,494,845 + CA265_RS17980 0.54 -0.3 4,494,846 - CA265_RS17980 0.54 +0.4 4,494,907 - CA265_RS17980 0.59 +0.4 4,494,907 - CA265_RS17980 0.59 -0.9
Or see this region's nucleotide sequence