Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS15880 and CA265_RS15885 are separated by 150 nucleotides CA265_RS15885 and CA265_RS15890 overlap by 8 nucleotides CA265_RS15890 and CA265_RS15895 are separated by 108 nucleotides CA265_RS15895 and CA265_RS15900 are separated by 80 nucleotides CA265_RS15900 and CA265_RS15905 overlap by 20 nucleotides
CA265_RS15880: CA265_RS15880 - sugar ABC transporter ATP-binding protein, at 3,993,115 to 3,994,107
_RS15880
CA265_RS15885: CA265_RS15885 - hypothetical protein, at 3,994,258 to 3,994,536
_RS15885
CA265_RS15890: CA265_RS15890 - DNA-binding protein, at 3,994,529 to 3,994,942
_RS15890
CA265_RS15895: CA265_RS15895 - hypothetical protein, at 3,995,051 to 3,995,836
_RS15895
CA265_RS15900: CA265_RS15900 - hypothetical protein, at 3,995,917 to 3,996,678
_RS15900
CA265_RS15905: CA265_RS15905 - RNA polymerase sigma factor, at 3,996,659 to 3,997,228
_RS15905
Position (kb) 3995
3996 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 3994.093 kb on + strand at 3994.112 kb on + strand at 3994.211 kb on + strand at 3994.212 kb on - strand at 3994.216 kb on - strand at 3994.229 kb on + strand at 3994.230 kb on - strand at 3994.539 kb on + strand at 3994.540 kb on - strand at 3994.555 kb on + strand at 3994.579 kb on + strand, within CA265_RS15890 at 3994.677 kb on - strand, within CA265_RS15890 at 3994.677 kb on - strand, within CA265_RS15890 at 3994.679 kb on + strand, within CA265_RS15890 at 3994.699 kb on + strand, within CA265_RS15890 at 3994.705 kb on + strand, within CA265_RS15890 at 3994.753 kb on - strand, within CA265_RS15890 at 3994.753 kb on - strand, within CA265_RS15890 at 3994.755 kb on + strand, within CA265_RS15890 at 3994.779 kb on + strand at 3994.856 kb on - strand, within CA265_RS15890 at 3994.862 kb on - strand, within CA265_RS15890 at 3994.862 kb on - strand, within CA265_RS15890 at 3994.863 kb on + strand, within CA265_RS15890 at 3994.864 kb on - strand, within CA265_RS15890 at 3994.880 kb on - strand, within CA265_RS15890 at 3994.903 kb on - strand at 3995.024 kb on - strand at 3995.206 kb on - strand, within CA265_RS15895 at 3995.209 kb on + strand, within CA265_RS15895 at 3995.361 kb on + strand, within CA265_RS15895 at 3995.444 kb on - strand, within CA265_RS15895 at 3995.490 kb on - strand, within CA265_RS15895 at 3995.493 kb on + strand, within CA265_RS15895 at 3995.572 kb on - strand, within CA265_RS15895 at 3995.621 kb on + strand, within CA265_RS15895 at 3995.755 kb on + strand, within CA265_RS15895 at 3995.857 kb on - strand at 3995.857 kb on - strand at 3995.866 kb on - strand at 3995.955 kb on + strand at 3996.187 kb on - strand, within CA265_RS15900 at 3996.187 kb on - strand, within CA265_RS15900 at 3996.457 kb on - strand, within CA265_RS15900 at 3996.548 kb on - strand, within CA265_RS15900 at 3996.548 kb on - strand, within CA265_RS15900 at 3996.796 kb on - strand, within CA265_RS15905 at 3996.806 kb on + strand, within CA265_RS15905 at 3996.810 kb on + strand, within CA265_RS15905 at 3996.810 kb on + strand, within CA265_RS15905 at 3996.810 kb on + strand, within CA265_RS15905 at 3996.811 kb on - strand, within CA265_RS15905 at 3996.811 kb on - strand, within CA265_RS15905 at 3996.811 kb on - strand, within CA265_RS15905 at 3996.811 kb on - strand, within CA265_RS15905 at 3996.811 kb on - strand, within CA265_RS15905
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 3,994,093 + -0.2 3,994,112 + +1.0 3,994,211 + +0.7 3,994,212 - -0.1 3,994,216 - +0.0 3,994,229 + -2.0 3,994,230 - -2.0 3,994,539 + +0.9 3,994,540 - -0.1 3,994,555 + +0.8 3,994,579 + CA265_RS15890 0.12 -1.0 3,994,677 - CA265_RS15890 0.36 -0.4 3,994,677 - CA265_RS15890 0.36 -0.9 3,994,679 + CA265_RS15890 0.36 +0.4 3,994,699 + CA265_RS15890 0.41 -0.2 3,994,705 + CA265_RS15890 0.43 +0.9 3,994,753 - CA265_RS15890 0.54 -0.0 3,994,753 - CA265_RS15890 0.54 -1.0 3,994,755 + CA265_RS15890 0.55 +0.3 3,994,779 + +1.0 3,994,856 - CA265_RS15890 0.79 +0.0 3,994,862 - CA265_RS15890 0.80 -0.2 3,994,862 - CA265_RS15890 0.80 -0.3 3,994,863 + CA265_RS15890 0.81 -0.4 3,994,864 - CA265_RS15890 0.81 +0.2 3,994,880 - CA265_RS15890 0.85 +0.3 3,994,903 - -0.1 3,995,024 - -0.9 3,995,206 - CA265_RS15895 0.20 +3.0 3,995,209 + CA265_RS15895 0.20 +3.8 3,995,361 + CA265_RS15895 0.39 +5.0 3,995,444 - CA265_RS15895 0.50 +4.0 3,995,490 - CA265_RS15895 0.56 +4.1 3,995,493 + CA265_RS15895 0.56 +3.2 3,995,572 - CA265_RS15895 0.66 +3.8 3,995,621 + CA265_RS15895 0.73 +4.9 3,995,755 + CA265_RS15895 0.90 +4.3 3,995,857 - +0.6 3,995,857 - +1.5 3,995,866 - -0.9 3,995,955 + +5.2 3,996,187 - CA265_RS15900 0.35 +4.4 3,996,187 - CA265_RS15900 0.35 +4.3 3,996,457 - CA265_RS15900 0.71 +1.0 3,996,548 - CA265_RS15900 0.83 +0.2 3,996,548 - CA265_RS15900 0.83 +1.3 3,996,796 - CA265_RS15905 0.24 -0.8 3,996,806 + CA265_RS15905 0.26 -0.1 3,996,810 + CA265_RS15905 0.26 +0.4 3,996,810 + CA265_RS15905 0.26 +0.3 3,996,810 + CA265_RS15905 0.26 +0.3 3,996,811 - CA265_RS15905 0.27 -1.3 3,996,811 - CA265_RS15905 0.27 -0.8 3,996,811 - CA265_RS15905 0.27 -0.8 3,996,811 - CA265_RS15905 0.27 -0.8 3,996,811 - CA265_RS15905 0.27 -0.4
Or see this region's nucleotide sequence