Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS15420 and CA265_RS15425 are separated by 290 nucleotides CA265_RS15425 and CA265_RS15430 are separated by 241 nucleotides CA265_RS15430 and CA265_RS15435 are separated by 173 nucleotides
CA265_RS15420: CA265_RS15420 - hypothetical protein, at 3,878,860 to 3,879,708
_RS15420
CA265_RS15425: CA265_RS15425 - phosphopyruvate hydratase, at 3,879,999 to 3,881,294
_RS15425
CA265_RS15430: CA265_RS15430 - septum formation initiator, at 3,881,536 to 3,881,835
_RS15430
CA265_RS15435: CA265_RS15435 - glycine transporter (from data) , at 3,882,009 to 3,882,623
_RS15435
Position (kb)
3879
3880
3881
3882 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 3879.103 kb on - strand, within CA265_RS15420 at 3879.103 kb on - strand, within CA265_RS15420 at 3879.155 kb on - strand, within CA265_RS15420 at 3879.161 kb on + strand, within CA265_RS15420 at 3879.170 kb on + strand, within CA265_RS15420 at 3879.170 kb on + strand, within CA265_RS15420 at 3879.229 kb on + strand, within CA265_RS15420 at 3879.238 kb on - strand, within CA265_RS15420 at 3879.258 kb on - strand, within CA265_RS15420 at 3879.263 kb on + strand, within CA265_RS15420 at 3879.263 kb on + strand, within CA265_RS15420 at 3879.264 kb on - strand, within CA265_RS15420 at 3879.264 kb on - strand, within CA265_RS15420 at 3879.279 kb on - strand, within CA265_RS15420 at 3879.292 kb on - strand, within CA265_RS15420 at 3879.347 kb on + strand, within CA265_RS15420 at 3879.351 kb on + strand, within CA265_RS15420 at 3879.352 kb on - strand, within CA265_RS15420 at 3879.384 kb on + strand, within CA265_RS15420 at 3879.384 kb on + strand, within CA265_RS15420 at 3879.396 kb on + strand, within CA265_RS15420 at 3879.421 kb on - strand, within CA265_RS15420 at 3879.458 kb on - strand, within CA265_RS15420 at 3879.458 kb on - strand, within CA265_RS15420 at 3879.459 kb on + strand, within CA265_RS15420 at 3879.476 kb on - strand, within CA265_RS15420 at 3879.597 kb on + strand, within CA265_RS15420 at 3879.598 kb on - strand, within CA265_RS15420 at 3879.632 kb on + strand at 3879.633 kb on - strand at 3879.666 kb on + strand at 3879.666 kb on + strand at 3879.669 kb on - strand at 3879.686 kb on + strand at 3879.752 kb on + strand at 3879.753 kb on - strand at 3879.757 kb on + strand at 3879.758 kb on - strand at 3879.761 kb on + strand at 3879.762 kb on - strand at 3879.824 kb on + strand at 3879.863 kb on - strand at 3879.875 kb on + strand at 3879.875 kb on + strand at 3879.881 kb on - strand at 3879.904 kb on - strand at 3879.923 kb on + strand at 3881.280 kb on + strand at 3881.280 kb on + strand at 3881.281 kb on - strand at 3881.300 kb on - strand at 3881.325 kb on - strand at 3881.428 kb on - strand at 3881.455 kb on + strand at 3881.506 kb on - strand at 3881.533 kb on + strand at 3881.833 kb on + strand at 3881.966 kb on + strand at 3881.967 kb on - strand at 3881.973 kb on + strand at 3882.030 kb on - strand at 3882.036 kb on - strand at 3882.151 kb on + strand, within CA265_RS15435 at 3882.152 kb on - strand, within CA265_RS15435 at 3882.152 kb on - strand, within CA265_RS15435 at 3882.207 kb on - strand, within CA265_RS15435 at 3882.248 kb on + strand, within CA265_RS15435 at 3882.277 kb on - strand, within CA265_RS15435 at 3882.289 kb on + strand, within CA265_RS15435
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 3,879,103 - CA265_RS15420 0.29 -0.5 3,879,103 - CA265_RS15420 0.29 -0.6 3,879,155 - CA265_RS15420 0.35 -0.2 3,879,161 + CA265_RS15420 0.35 -0.4 3,879,170 + CA265_RS15420 0.37 +0.7 3,879,170 + CA265_RS15420 0.37 +0.3 3,879,229 + CA265_RS15420 0.43 +1.9 3,879,238 - CA265_RS15420 0.45 -0.5 3,879,258 - CA265_RS15420 0.47 -1.5 3,879,263 + CA265_RS15420 0.47 -1.3 3,879,263 + CA265_RS15420 0.47 +0.7 3,879,264 - CA265_RS15420 0.48 -0.7 3,879,264 - CA265_RS15420 0.48 -1.6 3,879,279 - CA265_RS15420 0.49 -1.2 3,879,292 - CA265_RS15420 0.51 -0.2 3,879,347 + CA265_RS15420 0.57 -0.6 3,879,351 + CA265_RS15420 0.58 +0.3 3,879,352 - CA265_RS15420 0.58 -0.7 3,879,384 + CA265_RS15420 0.62 +0.4 3,879,384 + CA265_RS15420 0.62 +0.4 3,879,396 + CA265_RS15420 0.63 -2.0 3,879,421 - CA265_RS15420 0.66 -0.5 3,879,458 - CA265_RS15420 0.70 -0.4 3,879,458 - CA265_RS15420 0.70 -0.3 3,879,459 + CA265_RS15420 0.71 -1.8 3,879,476 - CA265_RS15420 0.73 -0.6 3,879,597 + CA265_RS15420 0.87 -0.2 3,879,598 - CA265_RS15420 0.87 +1.4 3,879,632 + -0.5 3,879,633 - -2.0 3,879,666 + +0.9 3,879,666 + -0.1 3,879,669 - -1.4 3,879,686 + +0.5 3,879,752 + -0.6 3,879,753 - -0.5 3,879,757 + -0.1 3,879,758 - +0.2 3,879,761 + +0.1 3,879,762 - -0.8 3,879,824 + -1.4 3,879,863 - +0.7 3,879,875 + -0.4 3,879,875 + +0.3 3,879,881 - +0.3 3,879,904 - +0.1 3,879,923 + +0.2 3,881,280 + +0.1 3,881,280 + -2.3 3,881,281 - -4.8 3,881,300 - -2.4 3,881,325 - -1.7 3,881,428 - -2.4 3,881,455 + -1.7 3,881,506 - -0.2 3,881,533 + -0.1 3,881,833 + -1.1 3,881,966 + +0.2 3,881,967 - -0.4 3,881,973 + -0.8 3,882,030 - -0.4 3,882,036 - +0.5 3,882,151 + CA265_RS15435 0.23 -0.7 3,882,152 - CA265_RS15435 0.23 +0.5 3,882,152 - CA265_RS15435 0.23 +0.1 3,882,207 - CA265_RS15435 0.32 -0.5 3,882,248 + CA265_RS15435 0.39 +0.6 3,882,277 - CA265_RS15435 0.44 +0.4 3,882,289 + CA265_RS15435 0.46 -0.3
Or see this region's nucleotide sequence