Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS14530 and CA265_RS14535 are separated by 138 nucleotides CA265_RS14535 and CA265_RS14540 are separated by 173 nucleotides CA265_RS14540 and CA265_RS14545 overlap by 1 nucleotides CA265_RS14545 and CA265_RS14550 are separated by 53 nucleotides
CA265_RS14530: CA265_RS14530 - nicotinate-nucleotide diphosphorylase (carboxylating), at 3,660,836 to 3,661,678
_RS14530
CA265_RS14535: CA265_RS14535 - DUF4783 domain-containing protein, at 3,661,817 to 3,662,215
_RS14535
CA265_RS14540: CA265_RS14540 - phosphoglycerate mutase (2,3-diphosphoglycerate-independent), at 3,662,389 to 3,663,912
_RS14540
CA265_RS14545: CA265_RS14545 - hypothetical protein, at 3,663,912 to 3,664,442
_RS14545
CA265_RS14550: CA265_RS14550 - hypothetical protein, at 3,664,496 to 3,665,116
_RS14550
Position (kb)
3662
3663
3664 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3661.414 kb on + strand, within CA265_RS14530 at 3661.475 kb on + strand, within CA265_RS14530 at 3661.691 kb on - strand at 3661.718 kb on - strand at 3661.730 kb on - strand at 3661.753 kb on - strand at 3661.757 kb on + strand at 3661.757 kb on + strand at 3661.759 kb on + strand at 3661.913 kb on - strand, within CA265_RS14535 at 3662.170 kb on - strand, within CA265_RS14535 at 3662.171 kb on + strand, within CA265_RS14535 at 3662.171 kb on + strand, within CA265_RS14535 at 3662.186 kb on - strand at 3662.202 kb on - strand at 3662.239 kb on - strand at 3662.245 kb on - strand at 3662.307 kb on + strand at 3662.318 kb on + strand at 3662.318 kb on + strand at 3662.344 kb on + strand at 3664.002 kb on + strand, within CA265_RS14545 at 3664.003 kb on - strand, within CA265_RS14545 at 3664.012 kb on + strand, within CA265_RS14545 at 3664.196 kb on - strand, within CA265_RS14545 at 3664.217 kb on + strand, within CA265_RS14545 at 3664.218 kb on - strand, within CA265_RS14545 at 3664.218 kb on - strand, within CA265_RS14545 at 3664.223 kb on + strand, within CA265_RS14545 at 3664.224 kb on - strand, within CA265_RS14545 at 3664.301 kb on + strand, within CA265_RS14545 at 3664.301 kb on + strand, within CA265_RS14545 at 3664.326 kb on + strand, within CA265_RS14545 at 3664.345 kb on + strand, within CA265_RS14545 at 3664.351 kb on - strand, within CA265_RS14545 at 3664.369 kb on - strand, within CA265_RS14545 at 3664.369 kb on - strand, within CA265_RS14545 at 3664.503 kb on - strand at 3664.547 kb on - strand at 3664.548 kb on + strand at 3664.557 kb on - strand at 3664.571 kb on + strand, within CA265_RS14550 at 3664.595 kb on - strand at 3664.677 kb on + strand, within CA265_RS14550 at 3664.682 kb on + strand, within CA265_RS14550 at 3664.724 kb on - strand, within CA265_RS14550 at 3664.783 kb on + strand, within CA265_RS14550 at 3664.783 kb on + strand, within CA265_RS14550 at 3664.783 kb on + strand, within CA265_RS14550 at 3664.783 kb on + strand, within CA265_RS14550 at 3664.784 kb on - strand, within CA265_RS14550 at 3664.839 kb on + strand, within CA265_RS14550 at 3664.894 kb on + strand, within CA265_RS14550 at 3664.894 kb on + strand, within CA265_RS14550
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 3,661,414 + CA265_RS14530 0.69 -2.0 3,661,475 + CA265_RS14530 0.76 -2.6 3,661,691 - +0.4 3,661,718 - +0.8 3,661,730 - -0.2 3,661,753 - +0.1 3,661,757 + +1.6 3,661,757 + +1.2 3,661,759 + +3.7 3,661,913 - CA265_RS14535 0.24 +2.2 3,662,170 - CA265_RS14535 0.88 +1.7 3,662,171 + CA265_RS14535 0.89 +3.2 3,662,171 + CA265_RS14535 0.89 +1.9 3,662,186 - +1.4 3,662,202 - +2.4 3,662,239 - +1.9 3,662,245 - +2.0 3,662,307 + +0.2 3,662,318 + +0.2 3,662,318 + +1.4 3,662,344 + +1.6 3,664,002 + CA265_RS14545 0.17 -0.5 3,664,003 - CA265_RS14545 0.17 +0.7 3,664,012 + CA265_RS14545 0.19 -0.2 3,664,196 - CA265_RS14545 0.53 +1.4 3,664,217 + CA265_RS14545 0.57 +1.7 3,664,218 - CA265_RS14545 0.58 -1.6 3,664,218 - CA265_RS14545 0.58 -0.1 3,664,223 + CA265_RS14545 0.59 -0.5 3,664,224 - CA265_RS14545 0.59 -0.5 3,664,301 + CA265_RS14545 0.73 +0.9 3,664,301 + CA265_RS14545 0.73 +1.0 3,664,326 + CA265_RS14545 0.78 -0.1 3,664,345 + CA265_RS14545 0.82 -0.0 3,664,351 - CA265_RS14545 0.83 -0.6 3,664,369 - CA265_RS14545 0.86 -0.5 3,664,369 - CA265_RS14545 0.86 -1.0 3,664,503 - -0.4 3,664,547 - +0.6 3,664,548 + -1.2 3,664,557 - +0.5 3,664,571 + CA265_RS14550 0.12 -0.4 3,664,595 - -0.9 3,664,677 + CA265_RS14550 0.29 +0.7 3,664,682 + CA265_RS14550 0.30 +0.2 3,664,724 - CA265_RS14550 0.37 +0.3 3,664,783 + CA265_RS14550 0.46 -0.3 3,664,783 + CA265_RS14550 0.46 +0.3 3,664,783 + CA265_RS14550 0.46 +0.4 3,664,783 + CA265_RS14550 0.46 +0.4 3,664,784 - CA265_RS14550 0.46 -0.9 3,664,839 + CA265_RS14550 0.55 -1.0 3,664,894 + CA265_RS14550 0.64 +0.0 3,664,894 + CA265_RS14550 0.64 +0.8
Or see this region's nucleotide sequence