Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS14475 and CA265_RS14480 are separated by 6 nucleotides CA265_RS14480 and CA265_RS14485 are separated by 109 nucleotides CA265_RS14485 and CA265_RS14490 are separated by 173 nucleotides CA265_RS14490 and CA265_RS14495 are separated by 38 nucleotides
CA265_RS14475: CA265_RS14475 - glutathione peroxidase, at 3,650,485 to 3,650,997
_RS14475
CA265_RS14480: CA265_RS14480 - hypothetical protein, at 3,651,004 to 3,651,618
_RS14480
CA265_RS14485: CA265_RS14485 - bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase, at 3,651,728 to 3,653,935
_RS14485
CA265_RS14490: CA265_RS14490 - transcriptional repressor, at 3,654,109 to 3,654,606
_RS14490
CA265_RS14495: CA265_RS14495 - adenylosuccinate synthase, at 3,654,645 to 3,655,925
_RS14495
Position (kb)
3651
3652
3653
3654 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3650.754 kb on + strand, within CA265_RS14475 at 3650.830 kb on - strand, within CA265_RS14475 at 3650.872 kb on - strand, within CA265_RS14475 at 3650.897 kb on - strand, within CA265_RS14475 at 3650.897 kb on - strand, within CA265_RS14475 at 3650.903 kb on - strand, within CA265_RS14475 at 3650.949 kb on + strand at 3650.972 kb on + strand at 3650.998 kb on + strand at 3651.000 kb on + strand at 3651.001 kb on - strand at 3651.001 kb on - strand at 3651.001 kb on - strand at 3651.005 kb on + strand at 3651.005 kb on + strand at 3651.006 kb on - strand at 3651.006 kb on - strand at 3651.049 kb on - strand at 3651.081 kb on + strand, within CA265_RS14480 at 3651.088 kb on + strand, within CA265_RS14480 at 3651.111 kb on - strand, within CA265_RS14480 at 3651.211 kb on - strand, within CA265_RS14480 at 3651.218 kb on - strand, within CA265_RS14480 at 3651.373 kb on + strand, within CA265_RS14480 at 3651.379 kb on - strand, within CA265_RS14480 at 3651.380 kb on + strand, within CA265_RS14480 at 3651.396 kb on - strand, within CA265_RS14480 at 3651.396 kb on - strand, within CA265_RS14480 at 3651.441 kb on + strand, within CA265_RS14480 at 3651.441 kb on + strand, within CA265_RS14480 at 3651.442 kb on - strand, within CA265_RS14480 at 3651.589 kb on - strand at 3651.654 kb on + strand at 3651.655 kb on - strand at 3651.677 kb on - strand at 3651.682 kb on - strand at 3651.695 kb on - strand at 3651.698 kb on + strand at 3651.699 kb on - strand at 3651.787 kb on + strand at 3651.814 kb on + strand at 3651.818 kb on + strand at 3651.883 kb on + strand at 3651.884 kb on - strand at 3651.908 kb on + strand at 3651.911 kb on - strand at 3651.922 kb on + strand at 3651.973 kb on + strand, within CA265_RS14485 at 3651.974 kb on - strand, within CA265_RS14485 at 3651.978 kb on + strand, within CA265_RS14485 at 3652.031 kb on - strand, within CA265_RS14485 at 3652.109 kb on - strand, within CA265_RS14485 at 3652.109 kb on - strand, within CA265_RS14485 at 3652.164 kb on + strand, within CA265_RS14485 at 3652.204 kb on + strand, within CA265_RS14485 at 3652.204 kb on + strand, within CA265_RS14485 at 3652.244 kb on - strand, within CA265_RS14485 at 3652.299 kb on + strand, within CA265_RS14485 at 3652.299 kb on + strand, within CA265_RS14485 at 3652.300 kb on - strand, within CA265_RS14485 at 3652.300 kb on - strand, within CA265_RS14485 at 3652.300 kb on - strand, within CA265_RS14485 at 3652.300 kb on - strand, within CA265_RS14485 at 3652.340 kb on + strand, within CA265_RS14485 at 3652.359 kb on - strand, within CA265_RS14485 at 3652.620 kb on - strand, within CA265_RS14485 at 3652.630 kb on - strand, within CA265_RS14485 at 3652.639 kb on - strand, within CA265_RS14485 at 3652.749 kb on + strand, within CA265_RS14485 at 3652.760 kb on - strand, within CA265_RS14485 at 3653.075 kb on - strand, within CA265_RS14485 at 3653.075 kb on - strand, within CA265_RS14485 at 3653.934 kb on - strand at 3653.937 kb on + strand at 3653.972 kb on - strand at 3654.035 kb on + strand at 3654.035 kb on + strand at 3654.080 kb on + strand at 3654.084 kb on + strand at 3654.614 kb on + strand at 3654.614 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 3,650,754 + CA265_RS14475 0.52 +1.4 3,650,830 - CA265_RS14475 0.67 -1.1 3,650,872 - CA265_RS14475 0.75 +0.5 3,650,897 - CA265_RS14475 0.80 +1.0 3,650,897 - CA265_RS14475 0.80 -0.3 3,650,903 - CA265_RS14475 0.81 +0.3 3,650,949 + +0.9 3,650,972 + +1.8 3,650,998 + -0.0 3,651,000 + +0.9 3,651,001 - +0.1 3,651,001 - -1.0 3,651,001 - -1.0 3,651,005 + +0.7 3,651,005 + +2.9 3,651,006 - -0.4 3,651,006 - -0.6 3,651,049 - -0.7 3,651,081 + CA265_RS14480 0.13 +0.5 3,651,088 + CA265_RS14480 0.14 +1.3 3,651,111 - CA265_RS14480 0.17 +1.4 3,651,211 - CA265_RS14480 0.34 -1.2 3,651,218 - CA265_RS14480 0.35 +0.3 3,651,373 + CA265_RS14480 0.60 +0.7 3,651,379 - CA265_RS14480 0.61 -0.6 3,651,380 + CA265_RS14480 0.61 +2.3 3,651,396 - CA265_RS14480 0.64 -0.7 3,651,396 - CA265_RS14480 0.64 +0.4 3,651,441 + CA265_RS14480 0.71 +2.0 3,651,441 + CA265_RS14480 0.71 +1.1 3,651,442 - CA265_RS14480 0.71 +1.1 3,651,589 - +0.8 3,651,654 + +1.4 3,651,655 - -1.6 3,651,677 - -2.1 3,651,682 - -1.1 3,651,695 - -1.1 3,651,698 + +1.8 3,651,699 - -2.6 3,651,787 + -1.8 3,651,814 + -1.4 3,651,818 + -1.9 3,651,883 + +0.1 3,651,884 - +1.9 3,651,908 + +0.4 3,651,911 - +2.2 3,651,922 + +0.9 3,651,973 + CA265_RS14485 0.11 -0.1 3,651,974 - CA265_RS14485 0.11 +2.2 3,651,978 + CA265_RS14485 0.11 -0.5 3,652,031 - CA265_RS14485 0.14 +2.1 3,652,109 - CA265_RS14485 0.17 +1.9 3,652,109 - CA265_RS14485 0.17 +2.0 3,652,164 + CA265_RS14485 0.20 -2.8 3,652,204 + CA265_RS14485 0.22 +1.5 3,652,204 + CA265_RS14485 0.22 +1.8 3,652,244 - CA265_RS14485 0.23 +2.9 3,652,299 + CA265_RS14485 0.26 +2.1 3,652,299 + CA265_RS14485 0.26 +1.5 3,652,300 - CA265_RS14485 0.26 +2.3 3,652,300 - CA265_RS14485 0.26 +2.1 3,652,300 - CA265_RS14485 0.26 +2.1 3,652,300 - CA265_RS14485 0.26 +2.5 3,652,340 + CA265_RS14485 0.28 +2.6 3,652,359 - CA265_RS14485 0.29 +2.9 3,652,620 - CA265_RS14485 0.40 +2.2 3,652,630 - CA265_RS14485 0.41 +2.4 3,652,639 - CA265_RS14485 0.41 +2.3 3,652,749 + CA265_RS14485 0.46 +1.8 3,652,760 - CA265_RS14485 0.47 +2.4 3,653,075 - CA265_RS14485 0.61 -1.0 3,653,075 - CA265_RS14485 0.61 -2.4 3,653,934 - -0.9 3,653,937 + -0.4 3,653,972 - -1.0 3,654,035 + +0.4 3,654,035 + +1.1 3,654,080 + -0.0 3,654,084 + -1.6 3,654,614 + +0.0 3,654,614 + +0.4
Or see this region's nucleotide sequence