Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS11660 and CA265_RS11665 are separated by 272 nucleotides CA265_RS11665 and CA265_RS11670 are separated by 170 nucleotides CA265_RS11670 and CA265_RS11675 are separated by 62 nucleotides
CA265_RS11660: CA265_RS11660 - hypothetical protein, at 2,889,804 to 2,892,119
_RS11660
CA265_RS11665: CA265_RS11665 - transketolase, at 2,892,392 to 2,894,812
_RS11665
CA265_RS11670: CA265_RS11670 - hypothetical protein, at 2,894,983 to 2,895,375
_RS11670
CA265_RS11675: CA265_RS11675 - aspartate aminotransferase, at 2,895,438 to 2,896,637
_RS11675
Position (kb)
2892
2893
2894
2895 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2891.422 kb on + strand, within CA265_RS11660 at 2891.444 kb on - strand, within CA265_RS11660 at 2891.454 kb on + strand, within CA265_RS11660 at 2891.466 kb on - strand, within CA265_RS11660 at 2891.466 kb on - strand, within CA265_RS11660 at 2891.509 kb on + strand, within CA265_RS11660 at 2891.510 kb on - strand, within CA265_RS11660 at 2891.510 kb on - strand, within CA265_RS11660 at 2891.510 kb on - strand, within CA265_RS11660 at 2891.510 kb on - strand, within CA265_RS11660 at 2891.558 kb on + strand, within CA265_RS11660 at 2891.565 kb on - strand, within CA265_RS11660 at 2891.567 kb on - strand, within CA265_RS11660 at 2891.567 kb on - strand, within CA265_RS11660 at 2891.567 kb on - strand, within CA265_RS11660 at 2891.634 kb on + strand, within CA265_RS11660 at 2891.647 kb on + strand, within CA265_RS11660 at 2891.647 kb on + strand, within CA265_RS11660 at 2891.658 kb on - strand, within CA265_RS11660 at 2891.666 kb on - strand, within CA265_RS11660 at 2891.667 kb on + strand, within CA265_RS11660 at 2891.668 kb on - strand, within CA265_RS11660 at 2891.686 kb on + strand, within CA265_RS11660 at 2891.687 kb on - strand, within CA265_RS11660 at 2891.687 kb on - strand, within CA265_RS11660 at 2891.703 kb on - strand, within CA265_RS11660 at 2891.786 kb on - strand, within CA265_RS11660 at 2891.790 kb on + strand, within CA265_RS11660 at 2891.790 kb on + strand, within CA265_RS11660 at 2891.791 kb on - strand, within CA265_RS11660 at 2891.803 kb on + strand, within CA265_RS11660 at 2891.862 kb on - strand, within CA265_RS11660 at 2891.869 kb on + strand, within CA265_RS11660 at 2891.883 kb on - strand, within CA265_RS11660 at 2892.142 kb on - strand at 2892.207 kb on - strand at 2892.214 kb on - strand at 2892.269 kb on - strand at 2892.342 kb on - strand at 2894.886 kb on - strand at 2894.928 kb on + strand at 2894.940 kb on - strand at 2894.969 kb on + strand at 2894.979 kb on + strand at 2894.979 kb on + strand at 2894.999 kb on + strand at 2895.024 kb on - strand, within CA265_RS11670 at 2895.035 kb on + strand, within CA265_RS11670 at 2895.035 kb on + strand, within CA265_RS11670 at 2895.035 kb on + strand, within CA265_RS11670 at 2895.048 kb on + strand, within CA265_RS11670 at 2895.121 kb on + strand, within CA265_RS11670 at 2895.134 kb on - strand, within CA265_RS11670 at 2895.163 kb on - strand, within CA265_RS11670 at 2895.192 kb on + strand, within CA265_RS11670 at 2895.193 kb on - strand, within CA265_RS11670 at 2895.193 kb on - strand, within CA265_RS11670 at 2895.198 kb on - strand, within CA265_RS11670 at 2895.201 kb on + strand, within CA265_RS11670 at 2895.205 kb on + strand, within CA265_RS11670 at 2895.244 kb on - strand, within CA265_RS11670 at 2895.244 kb on - strand, within CA265_RS11670 at 2895.259 kb on - strand, within CA265_RS11670 at 2895.279 kb on + strand, within CA265_RS11670 at 2895.327 kb on + strand, within CA265_RS11670 at 2895.392 kb on - strand at 2895.549 kb on - strand at 2895.565 kb on + strand, within CA265_RS11675 at 2895.586 kb on + strand, within CA265_RS11675 at 2895.626 kb on + strand, within CA265_RS11675 at 2895.676 kb on - strand, within CA265_RS11675 at 2895.680 kb on + strand at 2895.691 kb on - strand, within CA265_RS11675 at 2895.696 kb on - strand, within CA265_RS11675 at 2895.698 kb on + strand, within CA265_RS11675 at 2895.698 kb on + strand, within CA265_RS11675 at 2895.699 kb on - strand, within CA265_RS11675
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 2,891,422 + CA265_RS11660 0.70 -0.3 2,891,444 - CA265_RS11660 0.71 +0.6 2,891,454 + CA265_RS11660 0.71 -0.7 2,891,466 - CA265_RS11660 0.72 +2.2 2,891,466 - CA265_RS11660 0.72 +1.1 2,891,509 + CA265_RS11660 0.74 -0.4 2,891,510 - CA265_RS11660 0.74 -0.2 2,891,510 - CA265_RS11660 0.74 -0.5 2,891,510 - CA265_RS11660 0.74 +1.2 2,891,510 - CA265_RS11660 0.74 +0.1 2,891,558 + CA265_RS11660 0.76 +0.3 2,891,565 - CA265_RS11660 0.76 -0.3 2,891,567 - CA265_RS11660 0.76 +0.1 2,891,567 - CA265_RS11660 0.76 -0.1 2,891,567 - CA265_RS11660 0.76 +1.0 2,891,634 + CA265_RS11660 0.79 +0.1 2,891,647 + CA265_RS11660 0.80 +0.3 2,891,647 + CA265_RS11660 0.80 +0.1 2,891,658 - CA265_RS11660 0.80 +0.3 2,891,666 - CA265_RS11660 0.80 -0.4 2,891,667 + CA265_RS11660 0.80 -0.7 2,891,668 - CA265_RS11660 0.80 +0.4 2,891,686 + CA265_RS11660 0.81 +0.2 2,891,687 - CA265_RS11660 0.81 -2.1 2,891,687 - CA265_RS11660 0.81 +0.9 2,891,703 - CA265_RS11660 0.82 -0.3 2,891,786 - CA265_RS11660 0.86 +0.4 2,891,790 + CA265_RS11660 0.86 +0.7 2,891,790 + CA265_RS11660 0.86 +0.1 2,891,791 - CA265_RS11660 0.86 -0.3 2,891,803 + CA265_RS11660 0.86 -0.0 2,891,862 - CA265_RS11660 0.89 +0.1 2,891,869 + CA265_RS11660 0.89 -0.3 2,891,883 - CA265_RS11660 0.90 -0.6 2,892,142 - -1.7 2,892,207 - -1.2 2,892,214 - +0.7 2,892,269 - +1.4 2,892,342 - +0.1 2,894,886 - -0.1 2,894,928 + -0.0 2,894,940 - -3.5 2,894,969 + +0.4 2,894,979 + +0.6 2,894,979 + +0.0 2,894,999 + -2.3 2,895,024 - CA265_RS11670 0.10 -1.4 2,895,035 + CA265_RS11670 0.13 -2.0 2,895,035 + CA265_RS11670 0.13 -2.2 2,895,035 + CA265_RS11670 0.13 -0.8 2,895,048 + CA265_RS11670 0.17 +0.6 2,895,121 + CA265_RS11670 0.35 -1.2 2,895,134 - CA265_RS11670 0.38 -0.9 2,895,163 - CA265_RS11670 0.46 -2.9 2,895,192 + CA265_RS11670 0.53 +0.1 2,895,193 - CA265_RS11670 0.53 -2.0 2,895,193 - CA265_RS11670 0.53 -1.2 2,895,198 - CA265_RS11670 0.55 -3.6 2,895,201 + CA265_RS11670 0.55 -1.5 2,895,205 + CA265_RS11670 0.56 -2.0 2,895,244 - CA265_RS11670 0.66 -2.3 2,895,244 - CA265_RS11670 0.66 -3.9 2,895,259 - CA265_RS11670 0.70 -1.2 2,895,279 + CA265_RS11670 0.75 -1.9 2,895,327 + CA265_RS11670 0.88 -2.1 2,895,392 - -3.8 2,895,549 - +0.1 2,895,565 + CA265_RS11675 0.11 -0.3 2,895,586 + CA265_RS11675 0.12 +0.1 2,895,626 + CA265_RS11675 0.16 -0.4 2,895,676 - CA265_RS11675 0.20 -0.9 2,895,680 + +1.6 2,895,691 - CA265_RS11675 0.21 +0.5 2,895,696 - CA265_RS11675 0.21 -0.5 2,895,698 + CA265_RS11675 0.22 +0.0 2,895,698 + CA265_RS11675 0.22 +1.2 2,895,699 - CA265_RS11675 0.22 -0.4
Or see this region's nucleotide sequence