Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS11295 and CA265_RS11300 are separated by 513 nucleotides CA265_RS11300 and CA265_RS11305 are separated by 350 nucleotides
CA265_RS11295: CA265_RS11295 - Tat (twin-arginine translocation) pathway signal sequence containing protein, at 2,798,359 to 2,799,081
_RS11295
CA265_RS11300: CA265_RS11300 - N-acetylglucosamine kinase (EC 2.7.1.59) (from data) , at 2,799,595 to 2,800,536
_RS11300
CA265_RS11305: CA265_RS11305 - glucuronyl hydrolase, at 2,800,887 to 2,802,092
_RS11305
Position (kb)
2799
2800
2801 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2798.662 kb on - strand, within CA265_RS11295 at 2798.849 kb on - strand, within CA265_RS11295 at 2798.857 kb on + strand, within CA265_RS11295 at 2798.868 kb on + strand, within CA265_RS11295 at 2798.869 kb on - strand, within CA265_RS11295 at 2798.869 kb on - strand, within CA265_RS11295 at 2798.871 kb on - strand, within CA265_RS11295 at 2798.922 kb on + strand, within CA265_RS11295 at 2798.931 kb on + strand, within CA265_RS11295 at 2798.956 kb on - strand, within CA265_RS11295 at 2798.956 kb on - strand, within CA265_RS11295 at 2799.097 kb on - strand at 2799.142 kb on + strand at 2799.144 kb on + strand at 2799.145 kb on - strand at 2799.145 kb on - strand at 2799.149 kb on + strand at 2799.149 kb on + strand at 2799.150 kb on - strand at 2799.150 kb on - strand at 2799.150 kb on - strand at 2799.155 kb on + strand at 2799.166 kb on + strand at 2799.203 kb on - strand at 2799.345 kb on + strand at 2799.345 kb on + strand at 2799.345 kb on + strand at 2799.346 kb on - strand at 2799.354 kb on + strand at 2799.355 kb on - strand at 2799.486 kb on - strand at 2799.516 kb on - strand at 2799.602 kb on - strand at 2799.712 kb on - strand, within CA265_RS11300 at 2799.756 kb on - strand, within CA265_RS11300 at 2799.775 kb on + strand, within CA265_RS11300 at 2799.775 kb on + strand, within CA265_RS11300 at 2799.807 kb on + strand, within CA265_RS11300 at 2799.817 kb on + strand, within CA265_RS11300 at 2799.817 kb on + strand, within CA265_RS11300 at 2799.818 kb on - strand, within CA265_RS11300 at 2799.825 kb on + strand, within CA265_RS11300 at 2799.826 kb on - strand, within CA265_RS11300 at 2799.829 kb on - strand, within CA265_RS11300 at 2799.879 kb on - strand, within CA265_RS11300 at 2799.912 kb on - strand, within CA265_RS11300 at 2799.912 kb on - strand, within CA265_RS11300 at 2799.949 kb on - strand, within CA265_RS11300 at 2799.986 kb on + strand, within CA265_RS11300 at 2800.039 kb on + strand, within CA265_RS11300 at 2800.040 kb on - strand, within CA265_RS11300 at 2800.048 kb on - strand, within CA265_RS11300 at 2800.093 kb on - strand, within CA265_RS11300 at 2800.117 kb on + strand, within CA265_RS11300 at 2800.153 kb on - strand, within CA265_RS11300 at 2800.153 kb on - strand, within CA265_RS11300 at 2800.173 kb on - strand, within CA265_RS11300 at 2800.216 kb on + strand, within CA265_RS11300 at 2800.216 kb on + strand, within CA265_RS11300 at 2800.224 kb on + strand, within CA265_RS11300 at 2800.226 kb on + strand, within CA265_RS11300 at 2800.279 kb on + strand, within CA265_RS11300 at 2800.334 kb on - strand, within CA265_RS11300 at 2800.334 kb on - strand, within CA265_RS11300 at 2800.335 kb on + strand, within CA265_RS11300 at 2800.378 kb on - strand, within CA265_RS11300 at 2800.378 kb on - strand, within CA265_RS11300 at 2800.477 kb on + strand at 2800.523 kb on + strand at 2800.562 kb on + strand at 2800.563 kb on - strand at 2800.632 kb on + strand at 2800.633 kb on - strand at 2800.668 kb on - strand at 2800.669 kb on + strand at 2800.670 kb on - strand at 2800.722 kb on - strand at 2800.739 kb on + strand at 2800.749 kb on + strand at 2800.758 kb on + strand at 2800.758 kb on + strand at 2800.759 kb on - strand at 2800.862 kb on - strand at 2800.910 kb on - strand at 2801.014 kb on + strand, within CA265_RS11305 at 2801.034 kb on + strand, within CA265_RS11305 at 2801.132 kb on - strand, within CA265_RS11305 at 2801.142 kb on + strand, within CA265_RS11305 at 2801.143 kb on - strand, within CA265_RS11305 at 2801.207 kb on + strand, within CA265_RS11305 at 2801.243 kb on + strand, within CA265_RS11305 at 2801.250 kb on + strand, within CA265_RS11305 at 2801.260 kb on - strand, within CA265_RS11305 at 2801.264 kb on + strand, within CA265_RS11305 at 2801.265 kb on - strand, within CA265_RS11305 at 2801.354 kb on - strand, within CA265_RS11305 at 2801.412 kb on - strand, within CA265_RS11305 at 2801.431 kb on + strand, within CA265_RS11305 at 2801.433 kb on + strand, within CA265_RS11305 at 2801.536 kb on - strand, within CA265_RS11305
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 2,798,662 - CA265_RS11295 0.42 -0.9 2,798,849 - CA265_RS11295 0.68 +0.9 2,798,857 + CA265_RS11295 0.69 +0.6 2,798,868 + CA265_RS11295 0.70 +1.1 2,798,869 - CA265_RS11295 0.71 -0.9 2,798,869 - CA265_RS11295 0.71 -1.2 2,798,871 - CA265_RS11295 0.71 +0.6 2,798,922 + CA265_RS11295 0.78 -0.3 2,798,931 + CA265_RS11295 0.79 -1.7 2,798,956 - CA265_RS11295 0.83 +0.8 2,798,956 - CA265_RS11295 0.83 -0.8 2,799,097 - -0.3 2,799,142 + +0.3 2,799,144 + -0.2 2,799,145 - -0.5 2,799,145 - -1.4 2,799,149 + -0.4 2,799,149 + +1.1 2,799,150 - +0.0 2,799,150 - -0.6 2,799,150 - +0.2 2,799,155 + +1.1 2,799,166 + +1.5 2,799,203 - -1.8 2,799,345 + +0.4 2,799,345 + +0.9 2,799,345 + +0.4 2,799,346 - -0.7 2,799,354 + -1.6 2,799,355 - -1.0 2,799,486 - -0.5 2,799,516 - -2.0 2,799,602 - -0.1 2,799,712 - CA265_RS11300 0.12 -0.0 2,799,756 - CA265_RS11300 0.17 -0.1 2,799,775 + CA265_RS11300 0.19 +0.0 2,799,775 + CA265_RS11300 0.19 +0.5 2,799,807 + CA265_RS11300 0.23 +0.2 2,799,817 + CA265_RS11300 0.24 -0.8 2,799,817 + CA265_RS11300 0.24 +0.1 2,799,818 - CA265_RS11300 0.24 +1.1 2,799,825 + CA265_RS11300 0.24 -1.0 2,799,826 - CA265_RS11300 0.25 -1.1 2,799,829 - CA265_RS11300 0.25 +0.5 2,799,879 - CA265_RS11300 0.30 +0.2 2,799,912 - CA265_RS11300 0.34 +0.3 2,799,912 - CA265_RS11300 0.34 +0.4 2,799,949 - CA265_RS11300 0.38 +0.5 2,799,986 + CA265_RS11300 0.42 +1.4 2,800,039 + CA265_RS11300 0.47 -0.2 2,800,040 - CA265_RS11300 0.47 -0.0 2,800,048 - CA265_RS11300 0.48 -1.7 2,800,093 - CA265_RS11300 0.53 -0.3 2,800,117 + CA265_RS11300 0.55 -0.8 2,800,153 - CA265_RS11300 0.59 -0.5 2,800,153 - CA265_RS11300 0.59 -0.1 2,800,173 - CA265_RS11300 0.61 +0.3 2,800,216 + CA265_RS11300 0.66 +0.5 2,800,216 + CA265_RS11300 0.66 +0.3 2,800,224 + CA265_RS11300 0.67 +0.4 2,800,226 + CA265_RS11300 0.67 -0.1 2,800,279 + CA265_RS11300 0.73 +0.1 2,800,334 - CA265_RS11300 0.78 +1.7 2,800,334 - CA265_RS11300 0.78 +1.3 2,800,335 + CA265_RS11300 0.79 -0.2 2,800,378 - CA265_RS11300 0.83 -0.7 2,800,378 - CA265_RS11300 0.83 +0.8 2,800,477 + -1.1 2,800,523 + +0.4 2,800,562 + -0.2 2,800,563 - +0.9 2,800,632 + +0.5 2,800,633 - +0.3 2,800,668 - +0.2 2,800,669 + -0.2 2,800,670 - +0.7 2,800,722 - +0.8 2,800,739 + -0.8 2,800,749 + -0.6 2,800,758 + +0.3 2,800,758 + -0.3 2,800,759 - +0.3 2,800,862 - -0.0 2,800,910 - +0.4 2,801,014 + CA265_RS11305 0.11 +0.5 2,801,034 + CA265_RS11305 0.12 +0.4 2,801,132 - CA265_RS11305 0.20 -0.4 2,801,142 + CA265_RS11305 0.21 +1.2 2,801,143 - CA265_RS11305 0.21 +2.6 2,801,207 + CA265_RS11305 0.27 +0.9 2,801,243 + CA265_RS11305 0.30 +0.0 2,801,250 + CA265_RS11305 0.30 -1.1 2,801,260 - CA265_RS11305 0.31 +0.1 2,801,264 + CA265_RS11305 0.31 -0.5 2,801,265 - CA265_RS11305 0.31 +0.1 2,801,354 - CA265_RS11305 0.39 -0.2 2,801,412 - CA265_RS11305 0.44 -0.5 2,801,431 + CA265_RS11305 0.45 -0.6 2,801,433 + CA265_RS11305 0.45 +0.4 2,801,536 - CA265_RS11305 0.54 +0.1
Or see this region's nucleotide sequence