Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS10785 and CA265_RS10790 are separated by 117 nucleotides CA265_RS10790 and CA265_RS10795 are separated by 0 nucleotides CA265_RS10795 and CA265_RS10800 are separated by 76 nucleotides
CA265_RS10785: CA265_RS10785 - peptidoglycan synthetase, at 2,663,584 to 2,664,936
_RS10785
CA265_RS10790: CA265_RS10790 - 50S ribosomal protein L11 methyltransferase, at 2,665,054 to 2,665,896
_RS10790
CA265_RS10795: CA265_RS10795 - triose-phosphate isomerase, at 2,665,897 to 2,666,658
_RS10795
CA265_RS10800: CA265_RS10800 - glucose-1-phosphate thymidylyltransferase, at 2,666,735 to 2,667,901
_RS10800
Position (kb)
2665
2666
2667 Strain fitness (log2 ratio)
-2
-1
0
1 at 2664.928 kb on + strand at 2664.928 kb on + strand at 2664.929 kb on - strand at 2665.019 kb on - strand at 2665.041 kb on + strand at 2665.048 kb on - strand at 2665.112 kb on + strand at 2665.125 kb on - strand at 2665.208 kb on - strand, within CA265_RS10790 at 2665.243 kb on - strand, within CA265_RS10790 at 2665.249 kb on - strand, within CA265_RS10790 at 2665.336 kb on - strand, within CA265_RS10790 at 2665.380 kb on - strand, within CA265_RS10790 at 2665.453 kb on - strand, within CA265_RS10790 at 2665.555 kb on + strand, within CA265_RS10790 at 2665.555 kb on + strand, within CA265_RS10790 at 2665.565 kb on + strand, within CA265_RS10790 at 2665.684 kb on + strand, within CA265_RS10790 at 2665.685 kb on - strand, within CA265_RS10790 at 2665.689 kb on + strand, within CA265_RS10790 at 2665.690 kb on - strand, within CA265_RS10790 at 2665.691 kb on + strand, within CA265_RS10790 at 2665.713 kb on + strand, within CA265_RS10790 at 2665.715 kb on + strand, within CA265_RS10790 at 2665.756 kb on - strand, within CA265_RS10790 at 2665.792 kb on - strand, within CA265_RS10790 at 2665.792 kb on - strand, within CA265_RS10790 at 2665.873 kb on - strand at 2665.877 kb on - strand at 2665.890 kb on - strand at 2665.915 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 2,664,928 + -1.1 2,664,928 + +0.5 2,664,929 - +0.0 2,665,019 - -0.7 2,665,041 + -1.0 2,665,048 - +0.7 2,665,112 + +0.2 2,665,125 - +0.3 2,665,208 - CA265_RS10790 0.18 +0.3 2,665,243 - CA265_RS10790 0.22 -0.7 2,665,249 - CA265_RS10790 0.23 +0.2 2,665,336 - CA265_RS10790 0.33 -0.8 2,665,380 - CA265_RS10790 0.39 -2.2 2,665,453 - CA265_RS10790 0.47 -0.2 2,665,555 + CA265_RS10790 0.59 -1.9 2,665,555 + CA265_RS10790 0.59 -0.3 2,665,565 + CA265_RS10790 0.61 -0.3 2,665,684 + CA265_RS10790 0.75 -0.8 2,665,685 - CA265_RS10790 0.75 -0.1 2,665,689 + CA265_RS10790 0.75 -1.2 2,665,690 - CA265_RS10790 0.75 -0.8 2,665,691 + CA265_RS10790 0.76 +0.5 2,665,713 + CA265_RS10790 0.78 -0.8 2,665,715 + CA265_RS10790 0.78 -0.4 2,665,756 - CA265_RS10790 0.83 -1.2 2,665,792 - CA265_RS10790 0.88 +0.1 2,665,792 - CA265_RS10790 0.88 +0.1 2,665,873 - -0.6 2,665,877 - +0.1 2,665,890 - +0.6 2,665,915 - +1.0
Or see this region's nucleotide sequence