Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS08705 and CA265_RS08710 are separated by 416 nucleotides CA265_RS08710 and CA265_RS08715 are separated by 260 nucleotides
CA265_RS08705: CA265_RS08705 - glutamate synthase, at 2,180,332 to 2,181,798
_RS08705
CA265_RS08710: CA265_RS08710 - hypothetical protein, at 2,182,215 to 2,183,417
_RS08710
CA265_RS08715: CA265_RS08715 - metallophosphoesterase, at 2,183,678 to 2,184,781
_RS08715
Position (kb)
2182
2183
2184 Strain fitness (log2 ratio)
-1
0
1
2 at 2181.422 kb on + strand, within CA265_RS08705 at 2181.446 kb on + strand, within CA265_RS08705 at 2181.446 kb on + strand, within CA265_RS08705 at 2181.446 kb on + strand, within CA265_RS08705 at 2181.447 kb on - strand, within CA265_RS08705 at 2181.447 kb on - strand, within CA265_RS08705 at 2181.487 kb on - strand, within CA265_RS08705 at 2181.501 kb on + strand, within CA265_RS08705 at 2181.501 kb on + strand, within CA265_RS08705 at 2181.552 kb on - strand, within CA265_RS08705 at 2181.590 kb on + strand, within CA265_RS08705 at 2181.635 kb on - strand, within CA265_RS08705 at 2181.635 kb on - strand, within CA265_RS08705 at 2181.635 kb on - strand, within CA265_RS08705 at 2181.635 kb on - strand, within CA265_RS08705 at 2181.696 kb on - strand at 2181.746 kb on - strand at 2181.796 kb on + strand at 2181.860 kb on - strand at 2181.966 kb on + strand at 2181.986 kb on - strand at 2182.044 kb on - strand at 2182.093 kb on + strand at 2182.093 kb on + strand at 2182.155 kb on + strand at 2182.155 kb on + strand at 2182.156 kb on - strand at 2182.156 kb on - strand at 2182.156 kb on - strand at 2182.156 kb on - strand at 2182.513 kb on + strand, within CA265_RS08710 at 2182.608 kb on + strand, within CA265_RS08710 at 2182.608 kb on + strand, within CA265_RS08710 at 2182.660 kb on - strand, within CA265_RS08710 at 2182.660 kb on - strand, within CA265_RS08710 at 2182.661 kb on + strand, within CA265_RS08710 at 2182.661 kb on + strand, within CA265_RS08710 at 2182.661 kb on + strand, within CA265_RS08710 at 2182.732 kb on - strand, within CA265_RS08710 at 2182.779 kb on - strand, within CA265_RS08710 at 2182.779 kb on - strand, within CA265_RS08710 at 2182.793 kb on + strand, within CA265_RS08710 at 2182.973 kb on + strand, within CA265_RS08710 at 2182.979 kb on + strand, within CA265_RS08710 at 2182.987 kb on + strand, within CA265_RS08710 at 2182.987 kb on + strand, within CA265_RS08710 at 2183.016 kb on - strand, within CA265_RS08710 at 2183.016 kb on - strand, within CA265_RS08710 at 2183.018 kb on + strand, within CA265_RS08710 at 2183.018 kb on + strand, within CA265_RS08710 at 2183.119 kb on + strand, within CA265_RS08710 at 2183.141 kb on + strand, within CA265_RS08710 at 2183.144 kb on - strand, within CA265_RS08710 at 2183.144 kb on - strand, within CA265_RS08710 at 2183.274 kb on + strand, within CA265_RS08710 at 2183.284 kb on - strand, within CA265_RS08710 at 2183.308 kb on - strand at 2183.314 kb on - strand at 2183.381 kb on - strand at 2183.458 kb on + strand at 2183.491 kb on + strand at 2183.652 kb on + strand at 2183.675 kb on + strand at 2183.675 kb on + strand at 2183.675 kb on + strand at 2183.675 kb on + strand at 2183.676 kb on - strand at 2183.691 kb on + strand at 2183.786 kb on + strand at 2183.929 kb on + strand, within CA265_RS08715 at 2183.930 kb on - strand, within CA265_RS08715 at 2183.961 kb on + strand, within CA265_RS08715 at 2183.967 kb on - strand, within CA265_RS08715 at 2184.007 kb on - strand, within CA265_RS08715 at 2184.106 kb on - strand, within CA265_RS08715 at 2184.152 kb on + strand, within CA265_RS08715 at 2184.220 kb on + strand, within CA265_RS08715 at 2184.229 kb on - strand, within CA265_RS08715 at 2184.252 kb on + strand, within CA265_RS08715 at 2184.317 kb on + strand, within CA265_RS08715 at 2184.338 kb on - strand, within CA265_RS08715 at 2184.348 kb on - strand, within CA265_RS08715 at 2184.361 kb on + strand, within CA265_RS08715 at 2184.381 kb on + strand, within CA265_RS08715
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 2,181,422 + CA265_RS08705 0.74 +0.1 2,181,446 + CA265_RS08705 0.76 -0.0 2,181,446 + CA265_RS08705 0.76 +0.5 2,181,446 + CA265_RS08705 0.76 -0.1 2,181,447 - CA265_RS08705 0.76 -0.5 2,181,447 - CA265_RS08705 0.76 -1.2 2,181,487 - CA265_RS08705 0.79 -0.6 2,181,501 + CA265_RS08705 0.80 +2.1 2,181,501 + CA265_RS08705 0.80 +0.6 2,181,552 - CA265_RS08705 0.83 -0.3 2,181,590 + CA265_RS08705 0.86 +0.0 2,181,635 - CA265_RS08705 0.89 -0.6 2,181,635 - CA265_RS08705 0.89 -1.6 2,181,635 - CA265_RS08705 0.89 -1.2 2,181,635 - CA265_RS08705 0.89 -0.1 2,181,696 - -0.3 2,181,746 - -0.3 2,181,796 + +0.2 2,181,860 - +0.0 2,181,966 + -0.8 2,181,986 - +1.1 2,182,044 - +0.1 2,182,093 + +0.0 2,182,093 + +1.7 2,182,155 + -0.7 2,182,155 + +0.0 2,182,156 - +1.0 2,182,156 - -0.7 2,182,156 - -1.1 2,182,156 - -0.9 2,182,513 + CA265_RS08710 0.25 +0.2 2,182,608 + CA265_RS08710 0.33 -0.1 2,182,608 + CA265_RS08710 0.33 -0.5 2,182,660 - CA265_RS08710 0.37 -0.4 2,182,660 - CA265_RS08710 0.37 +1.6 2,182,661 + CA265_RS08710 0.37 +0.6 2,182,661 + CA265_RS08710 0.37 +1.2 2,182,661 + CA265_RS08710 0.37 +0.1 2,182,732 - CA265_RS08710 0.43 +0.6 2,182,779 - CA265_RS08710 0.47 +0.7 2,182,779 - CA265_RS08710 0.47 +0.9 2,182,793 + CA265_RS08710 0.48 -0.1 2,182,973 + CA265_RS08710 0.63 -0.1 2,182,979 + CA265_RS08710 0.64 +0.4 2,182,987 + CA265_RS08710 0.64 -0.9 2,182,987 + CA265_RS08710 0.64 +0.6 2,183,016 - CA265_RS08710 0.67 +1.2 2,183,016 - CA265_RS08710 0.67 +0.3 2,183,018 + CA265_RS08710 0.67 -1.2 2,183,018 + CA265_RS08710 0.67 -0.2 2,183,119 + CA265_RS08710 0.75 +0.2 2,183,141 + CA265_RS08710 0.77 +0.2 2,183,144 - CA265_RS08710 0.77 -0.1 2,183,144 - CA265_RS08710 0.77 +2.2 2,183,274 + CA265_RS08710 0.88 +0.5 2,183,284 - CA265_RS08710 0.89 +0.1 2,183,308 - -0.3 2,183,314 - -0.2 2,183,381 - +1.5 2,183,458 + +0.2 2,183,491 + -0.1 2,183,652 + +0.7 2,183,675 + -1.0 2,183,675 + +0.2 2,183,675 + -1.2 2,183,675 + -0.1 2,183,676 - +0.2 2,183,691 + +0.1 2,183,786 + +0.7 2,183,929 + CA265_RS08715 0.23 +0.5 2,183,930 - CA265_RS08715 0.23 +0.3 2,183,961 + CA265_RS08715 0.26 -1.3 2,183,967 - CA265_RS08715 0.26 +0.9 2,184,007 - CA265_RS08715 0.30 -0.2 2,184,106 - CA265_RS08715 0.39 +0.3 2,184,152 + CA265_RS08715 0.43 -0.2 2,184,220 + CA265_RS08715 0.49 +2.1 2,184,229 - CA265_RS08715 0.50 +1.3 2,184,252 + CA265_RS08715 0.52 -0.2 2,184,317 + CA265_RS08715 0.58 +0.1 2,184,338 - CA265_RS08715 0.60 -0.1 2,184,348 - CA265_RS08715 0.61 +0.9 2,184,361 + CA265_RS08715 0.62 -0.2 2,184,381 + CA265_RS08715 0.64 -1.0
Or see this region's nucleotide sequence