Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS06520 and CA265_RS06525 are separated by 3 nucleotides CA265_RS06525 and CA265_RS06530 are separated by 252 nucleotides CA265_RS06530 and CA265_RS06535 are separated by 348 nucleotides
CA265_RS06520: CA265_RS06520 - hypothetical protein, at 1,610,190 to 1,610,756
_RS06520
CA265_RS06525: CA265_RS06525 - hypothetical protein, at 1,610,760 to 1,611,308
_RS06525
CA265_RS06530: CA265_RS06530 - hypothetical protein, at 1,611,561 to 1,612,865
_RS06530
CA265_RS06535: CA265_RS06535 - hypothetical protein, at 1,613,214 to 1,614,185
_RS06535
Position (kb)
1611
1612
1613 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1610.600 kb on - strand, within CA265_RS06520 at 1610.622 kb on + strand, within CA265_RS06520 at 1610.737 kb on + strand at 1610.844 kb on - strand, within CA265_RS06525 at 1610.861 kb on + strand, within CA265_RS06525 at 1610.889 kb on + strand, within CA265_RS06525 at 1610.890 kb on - strand, within CA265_RS06525 at 1610.890 kb on - strand, within CA265_RS06525 at 1610.890 kb on - strand, within CA265_RS06525 at 1610.890 kb on - strand, within CA265_RS06525 at 1610.930 kb on - strand, within CA265_RS06525 at 1611.168 kb on + strand, within CA265_RS06525 at 1611.168 kb on + strand, within CA265_RS06525 at 1611.265 kb on + strand at 1611.323 kb on + strand at 1611.358 kb on + strand at 1611.380 kb on + strand at 1611.408 kb on - strand at 1611.419 kb on - strand at 1611.423 kb on - strand at 1611.431 kb on + strand at 1611.435 kb on + strand at 1611.436 kb on - strand at 1611.437 kb on + strand at 1611.437 kb on + strand at 1611.438 kb on - strand at 1611.441 kb on + strand at 1611.442 kb on - strand at 1611.444 kb on - strand at 1611.490 kb on + strand at 1611.655 kb on + strand at 1611.736 kb on + strand, within CA265_RS06530 at 1611.767 kb on + strand, within CA265_RS06530 at 1611.797 kb on + strand, within CA265_RS06530 at 1611.798 kb on - strand, within CA265_RS06530 at 1611.927 kb on - strand, within CA265_RS06530 at 1611.949 kb on + strand, within CA265_RS06530 at 1611.950 kb on - strand, within CA265_RS06530 at 1611.966 kb on - strand, within CA265_RS06530 at 1611.968 kb on + strand, within CA265_RS06530 at 1611.969 kb on - strand, within CA265_RS06530 at 1611.969 kb on - strand, within CA265_RS06530 at 1612.013 kb on - strand, within CA265_RS06530 at 1612.020 kb on - strand, within CA265_RS06530 at 1612.038 kb on - strand, within CA265_RS06530 at 1612.067 kb on + strand, within CA265_RS06530 at 1612.068 kb on - strand, within CA265_RS06530 at 1612.227 kb on - strand, within CA265_RS06530 at 1612.227 kb on - strand, within CA265_RS06530 at 1612.227 kb on - strand, within CA265_RS06530 at 1612.296 kb on - strand, within CA265_RS06530 at 1612.308 kb on + strand, within CA265_RS06530 at 1612.308 kb on + strand, within CA265_RS06530 at 1612.310 kb on + strand, within CA265_RS06530 at 1612.311 kb on - strand, within CA265_RS06530 at 1612.389 kb on - strand, within CA265_RS06530 at 1612.447 kb on + strand, within CA265_RS06530 at 1612.448 kb on - strand, within CA265_RS06530 at 1612.450 kb on - strand, within CA265_RS06530 at 1612.451 kb on + strand, within CA265_RS06530 at 1612.535 kb on - strand, within CA265_RS06530 at 1612.646 kb on + strand, within CA265_RS06530 at 1612.657 kb on + strand, within CA265_RS06530 at 1612.685 kb on + strand, within CA265_RS06530 at 1612.733 kb on + strand, within CA265_RS06530 at 1612.757 kb on + strand at 1612.758 kb on - strand at 1612.767 kb on + strand at 1612.768 kb on - strand at 1612.809 kb on + strand at 1612.837 kb on - strand at 1612.869 kb on - strand at 1612.892 kb on + strand at 1612.892 kb on + strand at 1612.920 kb on - strand at 1612.928 kb on + strand at 1613.002 kb on + strand at 1613.062 kb on - strand at 1613.062 kb on - strand at 1613.072 kb on - strand at 1613.156 kb on + strand at 1613.157 kb on - strand at 1613.168 kb on + strand at 1613.169 kb on - strand at 1613.169 kb on - strand at 1613.170 kb on + strand at 1613.195 kb on - strand at 1613.200 kb on - strand at 1613.207 kb on + strand at 1613.292 kb on + strand at 1613.294 kb on + strand at 1613.301 kb on + strand at 1613.366 kb on + strand at 1613.374 kb on - strand, within CA265_RS06535 at 1613.394 kb on - strand, within CA265_RS06535 at 1613.455 kb on + strand, within CA265_RS06535 at 1613.529 kb on + strand, within CA265_RS06535 at 1613.593 kb on + strand, within CA265_RS06535 at 1613.593 kb on + strand, within CA265_RS06535 at 1613.593 kb on + strand, within CA265_RS06535 at 1613.654 kb on - strand, within CA265_RS06535 at 1613.803 kb on - strand, within CA265_RS06535
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 1,610,600 - CA265_RS06520 0.72 +1.2 1,610,622 + CA265_RS06520 0.76 +0.5 1,610,737 + +0.3 1,610,844 - CA265_RS06525 0.15 +0.5 1,610,861 + CA265_RS06525 0.18 -0.2 1,610,889 + CA265_RS06525 0.23 -0.4 1,610,890 - CA265_RS06525 0.24 +0.5 1,610,890 - CA265_RS06525 0.24 +1.1 1,610,890 - CA265_RS06525 0.24 +0.1 1,610,890 - CA265_RS06525 0.24 -2.0 1,610,930 - CA265_RS06525 0.31 +1.6 1,611,168 + CA265_RS06525 0.74 +0.8 1,611,168 + CA265_RS06525 0.74 +0.3 1,611,265 + +0.8 1,611,323 + -0.5 1,611,358 + +1.1 1,611,380 + -1.0 1,611,408 - -0.2 1,611,419 - +0.7 1,611,423 - -0.3 1,611,431 + +0.7 1,611,435 + -0.1 1,611,436 - -0.6 1,611,437 + +0.4 1,611,437 + +0.4 1,611,438 - -1.2 1,611,441 + +0.3 1,611,442 - -0.5 1,611,444 - +0.6 1,611,490 + +0.2 1,611,655 + +0.3 1,611,736 + CA265_RS06530 0.13 -0.1 1,611,767 + CA265_RS06530 0.16 +0.2 1,611,797 + CA265_RS06530 0.18 +0.3 1,611,798 - CA265_RS06530 0.18 -0.0 1,611,927 - CA265_RS06530 0.28 +1.1 1,611,949 + CA265_RS06530 0.30 +2.0 1,611,950 - CA265_RS06530 0.30 +0.3 1,611,966 - CA265_RS06530 0.31 +0.8 1,611,968 + CA265_RS06530 0.31 -0.1 1,611,969 - CA265_RS06530 0.31 -0.8 1,611,969 - CA265_RS06530 0.31 +1.1 1,612,013 - CA265_RS06530 0.35 +1.6 1,612,020 - CA265_RS06530 0.35 +0.6 1,612,038 - CA265_RS06530 0.37 +1.2 1,612,067 + CA265_RS06530 0.39 -0.1 1,612,068 - CA265_RS06530 0.39 -0.7 1,612,227 - CA265_RS06530 0.51 +0.3 1,612,227 - CA265_RS06530 0.51 +0.8 1,612,227 - CA265_RS06530 0.51 +1.3 1,612,296 - CA265_RS06530 0.56 -0.6 1,612,308 + CA265_RS06530 0.57 -0.8 1,612,308 + CA265_RS06530 0.57 -0.2 1,612,310 + CA265_RS06530 0.57 -0.0 1,612,311 - CA265_RS06530 0.57 +0.1 1,612,389 - CA265_RS06530 0.63 +1.8 1,612,447 + CA265_RS06530 0.68 +0.8 1,612,448 - CA265_RS06530 0.68 +0.8 1,612,450 - CA265_RS06530 0.68 -1.1 1,612,451 + CA265_RS06530 0.68 -2.0 1,612,535 - CA265_RS06530 0.75 -1.4 1,612,646 + CA265_RS06530 0.83 -0.6 1,612,657 + CA265_RS06530 0.84 +0.3 1,612,685 + CA265_RS06530 0.86 +1.0 1,612,733 + CA265_RS06530 0.90 +1.7 1,612,757 + +0.4 1,612,758 - +0.8 1,612,767 + -0.2 1,612,768 - +0.3 1,612,809 + +0.8 1,612,837 - +0.9 1,612,869 - +0.8 1,612,892 + -0.2 1,612,892 + -0.6 1,612,920 - +0.4 1,612,928 + +0.1 1,613,002 + +0.3 1,613,062 - +0.1 1,613,062 - -1.4 1,613,072 - -0.1 1,613,156 + +0.1 1,613,157 - +0.3 1,613,168 + +0.4 1,613,169 - +0.5 1,613,169 - +0.8 1,613,170 + -0.6 1,613,195 - +0.4 1,613,200 - -0.0 1,613,207 + -0.2 1,613,292 + +0.2 1,613,294 + -0.2 1,613,301 + -0.2 1,613,366 + +1.6 1,613,374 - CA265_RS06535 0.16 -0.4 1,613,394 - CA265_RS06535 0.19 -0.0 1,613,455 + CA265_RS06535 0.25 -1.1 1,613,529 + CA265_RS06535 0.32 -0.4 1,613,593 + CA265_RS06535 0.39 -1.9 1,613,593 + CA265_RS06535 0.39 -0.1 1,613,593 + CA265_RS06535 0.39 -1.0 1,613,654 - CA265_RS06535 0.45 -0.5 1,613,803 - CA265_RS06535 0.61 +0.9
Or see this region's nucleotide sequence