Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS03115 and CA265_RS03120 are separated by 3 nucleotides CA265_RS03120 and CA265_RS03125 are separated by 431 nucleotides CA265_RS03125 and CA265_RS03130 are separated by 240 nucleotides
CA265_RS03115: CA265_RS03115 - peroxiredoxin, at 772,023 to 772,484
_RS03115
CA265_RS03120: CA265_RS03120 - disulfide bond formation protein DsbA, at 772,488 to 773,006
_RS03120
CA265_RS03125: CA265_RS03125 - hypothetical protein, at 773,438 to 773,908
_RS03125
CA265_RS03130: CA265_RS03130 - 4-hydroxyphenylpyruvate dioxygenase, at 774,149 to 775,279
_RS03130
Position (kb) 773
774 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 772.454 kb on - strand at 772.488 kb on - strand at 772.553 kb on - strand, within CA265_RS03120 at 772.588 kb on - strand, within CA265_RS03120 at 772.610 kb on + strand, within CA265_RS03120 at 772.736 kb on + strand, within CA265_RS03120 at 772.826 kb on - strand, within CA265_RS03120 at 772.869 kb on - strand, within CA265_RS03120 at 772.879 kb on + strand, within CA265_RS03120 at 773.004 kb on + strand at 773.004 kb on + strand at 773.041 kb on + strand at 773.091 kb on - strand at 773.091 kb on - strand at 773.106 kb on + strand at 773.236 kb on - strand at 773.254 kb on - strand at 773.293 kb on + strand at 773.336 kb on - strand at 773.405 kb on + strand at 773.430 kb on + strand at 773.451 kb on + strand at 773.452 kb on - strand at 773.634 kb on - strand, within CA265_RS03125 at 773.759 kb on - strand, within CA265_RS03125 at 773.927 kb on - strand at 773.937 kb on - strand at 774.004 kb on + strand at 774.080 kb on - strand at 774.086 kb on + strand at 774.195 kb on - strand at 774.235 kb on + strand at 774.299 kb on + strand, within CA265_RS03130 at 774.344 kb on + strand, within CA265_RS03130 at 774.347 kb on + strand, within CA265_RS03130 at 774.347 kb on + strand, within CA265_RS03130 at 774.348 kb on - strand, within CA265_RS03130 at 774.348 kb on - strand, within CA265_RS03130 at 774.391 kb on + strand, within CA265_RS03130 at 774.391 kb on + strand, within CA265_RS03130 at 774.392 kb on - strand, within CA265_RS03130 at 774.487 kb on + strand, within CA265_RS03130 at 774.487 kb on + strand, within CA265_RS03130 at 774.488 kb on - strand, within CA265_RS03130 at 774.510 kb on + strand, within CA265_RS03130 at 774.514 kb on - strand, within CA265_RS03130 at 774.522 kb on + strand, within CA265_RS03130 at 774.678 kb on - strand, within CA265_RS03130 at 774.723 kb on - strand, within CA265_RS03130 at 774.746 kb on - strand, within CA265_RS03130 at 774.746 kb on - strand, within CA265_RS03130 at 774.748 kb on - strand, within CA265_RS03130 at 774.749 kb on + strand, within CA265_RS03130 at 774.750 kb on - strand, within CA265_RS03130 at 774.817 kb on + strand, within CA265_RS03130 at 774.869 kb on - strand, within CA265_RS03130
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 772,454 - -0.5 772,488 - -2.2 772,553 - CA265_RS03120 0.13 -0.3 772,588 - CA265_RS03120 0.19 -0.8 772,610 + CA265_RS03120 0.24 -0.5 772,736 + CA265_RS03120 0.48 +0.5 772,826 - CA265_RS03120 0.65 -1.5 772,869 - CA265_RS03120 0.73 +0.4 772,879 + CA265_RS03120 0.75 -0.4 773,004 + +0.5 773,004 + +2.0 773,041 + +0.4 773,091 - +0.7 773,091 - +0.4 773,106 + -0.4 773,236 - +1.6 773,254 - -0.1 773,293 + -0.1 773,336 - +2.4 773,405 + +0.4 773,430 + +0.4 773,451 + +0.5 773,452 - +1.0 773,634 - CA265_RS03125 0.42 +1.6 773,759 - CA265_RS03125 0.68 +0.4 773,927 - -0.4 773,937 - -3.0 774,004 + -0.4 774,080 - +0.5 774,086 + +0.9 774,195 - +1.2 774,235 + +1.5 774,299 + CA265_RS03130 0.13 +0.2 774,344 + CA265_RS03130 0.17 -0.6 774,347 + CA265_RS03130 0.18 -1.0 774,347 + CA265_RS03130 0.18 -0.2 774,348 - CA265_RS03130 0.18 +0.6 774,348 - CA265_RS03130 0.18 +0.4 774,391 + CA265_RS03130 0.21 -0.3 774,391 + CA265_RS03130 0.21 -0.4 774,392 - CA265_RS03130 0.21 +0.9 774,487 + CA265_RS03130 0.30 -0.8 774,487 + CA265_RS03130 0.30 -1.2 774,488 - CA265_RS03130 0.30 +0.4 774,510 + CA265_RS03130 0.32 -0.4 774,514 - CA265_RS03130 0.32 +0.8 774,522 + CA265_RS03130 0.33 -0.5 774,678 - CA265_RS03130 0.47 +0.4 774,723 - CA265_RS03130 0.51 +0.5 774,746 - CA265_RS03130 0.53 -0.5 774,746 - CA265_RS03130 0.53 +0.3 774,748 - CA265_RS03130 0.53 +0.2 774,749 + CA265_RS03130 0.53 -0.1 774,750 - CA265_RS03130 0.53 +0.9 774,817 + CA265_RS03130 0.59 +1.0 774,869 - CA265_RS03130 0.64 +0.8
Or see this region's nucleotide sequence