Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS02865 and CA265_RS02870 are separated by 82 nucleotides CA265_RS02870 and CA265_RS02875 are separated by 109 nucleotides CA265_RS02875 and CA265_RS02880 are separated by 9 nucleotides CA265_RS02880 and CA265_RS02885 are separated by 375 nucleotides CA265_RS02885 and CA265_RS02890 are separated by 2 nucleotides
CA265_RS02865: CA265_RS02865 - elongation factor Tu, at 714,719 to 715,906
_RS02865
CA265_RS02870: CA265_RS02870 - tRNA-Trp, at 715,989 to 716,059
_RS02870
CA265_RS02875: CA265_RS02875 - preprotein translocase subunit SecE, at 716,169 to 716,366
_RS02875
CA265_RS02880: CA265_RS02880 - transcription termination/antitermination factor NusG, at 716,376 to 716,915
_RS02880
CA265_RS02885: CA265_RS02885 - hypothetical protein, at 717,291 to 717,800
_RS02885
CA265_RS02890: CA265_RS02890 - hypothetical protein, at 717,803 to 718,321
_RS02890
Position (kb) 716
717 Strain fitness (log2 ratio) -1
0
1
2 at 717.027 kb on - strand at 717.053 kb on - strand at 717.056 kb on + strand at 717.056 kb on + strand at 717.056 kb on + strand at 717.057 kb on - strand at 717.074 kb on - strand at 717.083 kb on + strand at 717.124 kb on + strand at 717.124 kb on + strand at 717.136 kb on - strand at 717.136 kb on - strand at 717.136 kb on - strand at 717.274 kb on - strand at 717.274 kb on - strand at 717.274 kb on - strand at 717.346 kb on + strand, within CA265_RS02885 at 717.346 kb on + strand, within CA265_RS02885 at 717.346 kb on + strand, within CA265_RS02885 at 717.349 kb on + strand, within CA265_RS02885 at 717.349 kb on + strand, within CA265_RS02885 at 717.402 kb on - strand, within CA265_RS02885 at 717.407 kb on + strand, within CA265_RS02885 at 717.407 kb on + strand, within CA265_RS02885 at 717.408 kb on - strand, within CA265_RS02885 at 717.429 kb on - strand, within CA265_RS02885 at 717.502 kb on + strand, within CA265_RS02885 at 717.503 kb on - strand, within CA265_RS02885 at 717.540 kb on + strand, within CA265_RS02885 at 717.542 kb on + strand, within CA265_RS02885 at 717.542 kb on + strand, within CA265_RS02885 at 717.574 kb on + strand, within CA265_RS02885 at 717.575 kb on - strand, within CA265_RS02885 at 717.575 kb on - strand, within CA265_RS02885 at 717.575 kb on - strand, within CA265_RS02885 at 717.601 kb on - strand, within CA265_RS02885 at 717.610 kb on - strand, within CA265_RS02885 at 717.616 kb on + strand, within CA265_RS02885 at 717.616 kb on + strand, within CA265_RS02885 at 717.663 kb on - strand, within CA265_RS02885 at 717.663 kb on - strand, within CA265_RS02885 at 717.664 kb on + strand, within CA265_RS02885 at 717.774 kb on - strand at 717.834 kb on + strand at 717.865 kb on - strand, within CA265_RS02890
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 717,027 - +0.7 717,053 - +1.1 717,056 + +1.9 717,056 + +0.5 717,056 + +0.2 717,057 - -0.1 717,074 - +0.1 717,083 + -0.2 717,124 + -0.0 717,124 + +1.0 717,136 - -0.3 717,136 - -0.1 717,136 - +0.5 717,274 - -0.2 717,274 - +1.2 717,274 - +1.0 717,346 + CA265_RS02885 0.11 -0.3 717,346 + CA265_RS02885 0.11 +1.1 717,346 + CA265_RS02885 0.11 +0.9 717,349 + CA265_RS02885 0.11 +0.2 717,349 + CA265_RS02885 0.11 -0.1 717,402 - CA265_RS02885 0.22 +0.1 717,407 + CA265_RS02885 0.23 +0.2 717,407 + CA265_RS02885 0.23 -0.3 717,408 - CA265_RS02885 0.23 -0.3 717,429 - CA265_RS02885 0.27 -0.8 717,502 + CA265_RS02885 0.41 -0.2 717,503 - CA265_RS02885 0.42 -0.8 717,540 + CA265_RS02885 0.49 +0.3 717,542 + CA265_RS02885 0.49 -0.8 717,542 + CA265_RS02885 0.49 -0.3 717,574 + CA265_RS02885 0.55 +0.6 717,575 - CA265_RS02885 0.56 -0.9 717,575 - CA265_RS02885 0.56 -0.1 717,575 - CA265_RS02885 0.56 -1.2 717,601 - CA265_RS02885 0.61 -0.4 717,610 - CA265_RS02885 0.63 -0.3 717,616 + CA265_RS02885 0.64 -0.5 717,616 + CA265_RS02885 0.64 +0.2 717,663 - CA265_RS02885 0.73 -0.1 717,663 - CA265_RS02885 0.73 -0.5 717,664 + CA265_RS02885 0.73 +0.5 717,774 - -0.0 717,834 + +2.1 717,865 - CA265_RS02890 0.12 -0.3
Or see this region's nucleotide sequence