Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS01875 and CA265_RS01880 are separated by 5 nucleotides CA265_RS01880 and CA265_RS01885 are separated by 289 nucleotides
CA265_RS01875: CA265_RS01875 - hypothetical protein, at 468,592 to 469,029
_RS01875
CA265_RS01880: CA265_RS01880 - phosphoglycerate kinase, at 469,035 to 470,228
_RS01880
CA265_RS01885: CA265_RS01885 - hypothetical protein, at 470,518 to 471,492
_RS01885
Position (kb)
469
470
471 Strain fitness (log2 ratio)
-1
0
1
2
3 at 468.117 kb on + strand at 468.194 kb on + strand at 468.286 kb on + strand at 468.286 kb on + strand at 468.287 kb on - strand at 468.288 kb on + strand at 468.288 kb on + strand at 468.289 kb on - strand at 468.289 kb on - strand at 468.289 kb on - strand at 468.362 kb on + strand at 468.534 kb on - strand at 468.537 kb on - strand at 468.578 kb on - strand at 468.578 kb on - strand at 468.620 kb on + strand at 468.620 kb on + strand at 468.719 kb on + strand, within CA265_RS01875 at 468.810 kb on + strand, within CA265_RS01875 at 468.810 kb on + strand, within CA265_RS01875 at 468.885 kb on + strand, within CA265_RS01875 at 468.906 kb on + strand, within CA265_RS01875 at 468.975 kb on + strand, within CA265_RS01875 at 470.294 kb on + strand at 470.307 kb on + strand at 470.308 kb on - strand at 470.353 kb on - strand at 470.358 kb on + strand at 470.371 kb on + strand at 470.372 kb on - strand at 470.373 kb on + strand at 470.373 kb on + strand at 470.374 kb on - strand at 470.381 kb on - strand at 470.426 kb on + strand at 470.433 kb on + strand at 470.434 kb on - strand at 470.502 kb on + strand at 470.503 kb on - strand at 470.517 kb on + strand at 470.599 kb on - strand at 470.599 kb on - strand at 470.661 kb on + strand, within CA265_RS01885 at 470.722 kb on - strand, within CA265_RS01885 at 470.732 kb on + strand, within CA265_RS01885 at 470.733 kb on - strand, within CA265_RS01885 at 470.737 kb on + strand, within CA265_RS01885 at 470.749 kb on - strand, within CA265_RS01885 at 470.781 kb on - strand, within CA265_RS01885 at 470.915 kb on - strand, within CA265_RS01885 at 470.917 kb on - strand, within CA265_RS01885 at 470.956 kb on - strand, within CA265_RS01885 at 470.956 kb on - strand, within CA265_RS01885 at 470.997 kb on + strand, within CA265_RS01885 at 471.136 kb on + strand, within CA265_RS01885 at 471.164 kb on - strand, within CA265_RS01885 at 471.168 kb on - strand, within CA265_RS01885 at 471.198 kb on + strand, within CA265_RS01885
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 468,117 + +0.8 468,194 + +2.4 468,286 + -0.3 468,286 + -1.2 468,287 - +1.1 468,288 + -0.8 468,288 + -0.4 468,289 - -0.7 468,289 - +1.4 468,289 - +0.3 468,362 + -1.5 468,534 - -0.1 468,537 - -0.9 468,578 - -1.0 468,578 - -0.2 468,620 + +0.1 468,620 + +0.7 468,719 + CA265_RS01875 0.29 +0.8 468,810 + CA265_RS01875 0.50 -0.2 468,810 + CA265_RS01875 0.50 +0.2 468,885 + CA265_RS01875 0.67 +2.0 468,906 + CA265_RS01875 0.72 +0.9 468,975 + CA265_RS01875 0.87 +1.2 470,294 + -0.2 470,307 + -1.7 470,308 - -0.4 470,353 - -0.2 470,358 + +0.4 470,371 + +1.3 470,372 - +1.1 470,373 + +0.8 470,373 + +0.5 470,374 - +0.3 470,381 - -0.1 470,426 + +0.1 470,433 + +3.4 470,434 - +0.0 470,502 + +0.1 470,503 - +0.5 470,517 + +0.1 470,599 - +0.7 470,599 - +1.4 470,661 + CA265_RS01885 0.15 +0.9 470,722 - CA265_RS01885 0.21 -0.0 470,732 + CA265_RS01885 0.22 -0.5 470,733 - CA265_RS01885 0.22 +0.4 470,737 + CA265_RS01885 0.22 +1.0 470,749 - CA265_RS01885 0.24 -0.2 470,781 - CA265_RS01885 0.27 -0.0 470,915 - CA265_RS01885 0.41 +1.5 470,917 - CA265_RS01885 0.41 +0.4 470,956 - CA265_RS01885 0.45 -0.6 470,956 - CA265_RS01885 0.45 -0.4 470,997 + CA265_RS01885 0.49 +0.3 471,136 + CA265_RS01885 0.63 -1.1 471,164 - CA265_RS01885 0.66 +0.3 471,168 - CA265_RS01885 0.67 +0.5 471,198 + CA265_RS01885 0.70 -0.2
Or see this region's nucleotide sequence