Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS00720 and CA265_RS00725 are separated by 41 nucleotides CA265_RS00725 and CA265_RS00730 are separated by 97 nucleotides
CA265_RS00720: CA265_RS00720 - hypothetical protein, at 168,786 to 170,468
_RS00720
CA265_RS00725: CA265_RS00725 - hypothetical protein, at 170,510 to 173,620
_RS00725
CA265_RS00730: CA265_RS00730 - hypothetical protein, at 173,718 to 175,469
_RS00730
Position (kb) 170
171
172
173
174 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 169.547 kb on - strand, within CA265_RS00720 at 169.583 kb on + strand, within CA265_RS00720 at 169.682 kb on + strand, within CA265_RS00720 at 169.682 kb on + strand, within CA265_RS00720 at 169.683 kb on - strand, within CA265_RS00720 at 169.689 kb on - strand, within CA265_RS00720 at 169.789 kb on - strand, within CA265_RS00720 at 169.789 kb on - strand, within CA265_RS00720 at 169.790 kb on + strand, within CA265_RS00720 at 169.790 kb on + strand, within CA265_RS00720 at 169.806 kb on + strand, within CA265_RS00720 at 169.873 kb on + strand, within CA265_RS00720 at 169.885 kb on - strand, within CA265_RS00720 at 169.918 kb on + strand, within CA265_RS00720 at 169.944 kb on + strand, within CA265_RS00720 at 169.944 kb on + strand, within CA265_RS00720 at 170.025 kb on + strand, within CA265_RS00720 at 170.265 kb on - strand, within CA265_RS00720 at 170.266 kb on + strand, within CA265_RS00720 at 170.266 kb on + strand, within CA265_RS00720 at 170.267 kb on - strand, within CA265_RS00720 at 170.267 kb on - strand, within CA265_RS00720 at 170.267 kb on - strand, within CA265_RS00720 at 170.267 kb on - strand, within CA265_RS00720 at 170.276 kb on + strand, within CA265_RS00720 at 170.352 kb on + strand at 170.353 kb on - strand at 170.401 kb on - strand at 170.401 kb on - strand at 170.503 kb on - strand at 170.503 kb on - strand at 170.510 kb on - strand at 170.523 kb on + strand at 170.532 kb on + strand at 170.532 kb on + strand at 170.584 kb on + strand at 170.585 kb on - strand at 170.596 kb on - strand at 170.626 kb on + strand at 170.646 kb on + strand at 170.719 kb on + strand at 170.725 kb on + strand at 170.726 kb on - strand at 170.778 kb on + strand at 170.778 kb on + strand at 170.790 kb on + strand at 170.800 kb on + strand at 170.927 kb on - strand, within CA265_RS00725 at 171.019 kb on + strand, within CA265_RS00725 at 171.023 kb on + strand, within CA265_RS00725 at 171.024 kb on - strand, within CA265_RS00725 at 171.024 kb on - strand, within CA265_RS00725 at 171.034 kb on + strand, within CA265_RS00725 at 171.046 kb on - strand, within CA265_RS00725 at 171.075 kb on - strand, within CA265_RS00725 at 171.075 kb on - strand, within CA265_RS00725 at 171.075 kb on - strand, within CA265_RS00725 at 171.160 kb on + strand, within CA265_RS00725 at 171.193 kb on + strand, within CA265_RS00725 at 171.340 kb on + strand, within CA265_RS00725 at 171.341 kb on - strand, within CA265_RS00725 at 171.445 kb on + strand, within CA265_RS00725 at 171.452 kb on - strand, within CA265_RS00725 at 171.452 kb on - strand, within CA265_RS00725 at 171.452 kb on - strand, within CA265_RS00725 at 171.482 kb on - strand, within CA265_RS00725 at 171.482 kb on - strand, within CA265_RS00725 at 171.516 kb on - strand, within CA265_RS00725 at 171.538 kb on - strand, within CA265_RS00725 at 171.538 kb on - strand, within CA265_RS00725 at 171.538 kb on - strand, within CA265_RS00725 at 171.582 kb on - strand, within CA265_RS00725 at 171.593 kb on - strand, within CA265_RS00725 at 171.634 kb on - strand, within CA265_RS00725 at 171.634 kb on - strand, within CA265_RS00725 at 171.638 kb on - strand, within CA265_RS00725 at 171.652 kb on - strand, within CA265_RS00725 at 171.707 kb on + strand, within CA265_RS00725 at 171.708 kb on - strand, within CA265_RS00725 at 171.710 kb on + strand, within CA265_RS00725 at 171.759 kb on + strand, within CA265_RS00725 at 171.806 kb on + strand, within CA265_RS00725 at 171.807 kb on - strand, within CA265_RS00725 at 171.820 kb on - strand, within CA265_RS00725 at 171.866 kb on + strand, within CA265_RS00725 at 171.896 kb on - strand, within CA265_RS00725 at 171.954 kb on + strand, within CA265_RS00725 at 171.954 kb on + strand, within CA265_RS00725 at 171.955 kb on - strand, within CA265_RS00725 at 171.955 kb on - strand, within CA265_RS00725 at 171.958 kb on - strand, within CA265_RS00725 at 171.961 kb on - strand, within CA265_RS00725 at 171.961 kb on - strand, within CA265_RS00725 at 171.990 kb on + strand, within CA265_RS00725 at 171.990 kb on + strand, within CA265_RS00725 at 171.991 kb on - strand, within CA265_RS00725 at 171.991 kb on - strand, within CA265_RS00725 at 171.991 kb on - strand, within CA265_RS00725 at 171.991 kb on - strand, within CA265_RS00725 at 172.011 kb on + strand, within CA265_RS00725 at 172.017 kb on - strand, within CA265_RS00725 at 172.019 kb on + strand, within CA265_RS00725 at 172.020 kb on - strand, within CA265_RS00725 at 172.056 kb on + strand, within CA265_RS00725 at 172.056 kb on + strand, within CA265_RS00725 at 172.071 kb on - strand, within CA265_RS00725 at 172.097 kb on + strand, within CA265_RS00725 at 172.105 kb on + strand, within CA265_RS00725 at 172.105 kb on + strand, within CA265_RS00725 at 172.106 kb on - strand, within CA265_RS00725 at 172.125 kb on + strand, within CA265_RS00725 at 172.125 kb on + strand, within CA265_RS00725 at 172.126 kb on - strand, within CA265_RS00725 at 172.130 kb on - strand, within CA265_RS00725 at 172.176 kb on + strand, within CA265_RS00725 at 172.193 kb on + strand, within CA265_RS00725 at 172.193 kb on + strand, within CA265_RS00725 at 172.223 kb on - strand, within CA265_RS00725 at 172.225 kb on + strand, within CA265_RS00725 at 172.225 kb on + strand, within CA265_RS00725 at 172.226 kb on - strand, within CA265_RS00725 at 172.292 kb on + strand, within CA265_RS00725 at 172.355 kb on - strand, within CA265_RS00725 at 172.433 kb on - strand, within CA265_RS00725 at 172.472 kb on + strand, within CA265_RS00725 at 172.473 kb on - strand, within CA265_RS00725 at 172.478 kb on - strand, within CA265_RS00725 at 172.478 kb on - strand, within CA265_RS00725 at 172.528 kb on + strand, within CA265_RS00725 at 172.529 kb on - strand, within CA265_RS00725 at 172.529 kb on - strand, within CA265_RS00725 at 172.567 kb on + strand, within CA265_RS00725 at 172.594 kb on + strand, within CA265_RS00725 at 172.615 kb on - strand, within CA265_RS00725 at 172.666 kb on + strand, within CA265_RS00725 at 172.792 kb on + strand, within CA265_RS00725 at 172.792 kb on + strand, within CA265_RS00725 at 172.820 kb on + strand, within CA265_RS00725 at 172.821 kb on - strand, within CA265_RS00725 at 172.933 kb on + strand, within CA265_RS00725 at 173.198 kb on + strand, within CA265_RS00725 at 173.207 kb on - strand, within CA265_RS00725 at 173.207 kb on - strand, within CA265_RS00725 at 173.207 kb on - strand, within CA265_RS00725 at 173.207 kb on - strand, within CA265_RS00725 at 173.296 kb on + strand, within CA265_RS00725 at 173.296 kb on + strand, within CA265_RS00725 at 173.297 kb on - strand, within CA265_RS00725 at 173.302 kb on + strand, within CA265_RS00725 at 173.387 kb on + strand at 173.387 kb on + strand at 173.390 kb on + strand at 173.390 kb on + strand at 173.473 kb on - strand at 173.530 kb on - strand at 173.563 kb on - strand at 173.594 kb on - strand at 173.611 kb on + strand at 173.611 kb on + strand at 173.673 kb on - strand at 173.683 kb on + strand at 173.756 kb on + strand at 173.791 kb on + strand at 173.813 kb on + strand at 173.832 kb on - strand at 173.846 kb on + strand at 173.848 kb on + strand at 173.848 kb on + strand at 173.848 kb on + strand at 173.849 kb on - strand at 173.849 kb on - strand at 173.849 kb on - strand at 173.849 kb on - strand at 173.877 kb on + strand at 173.878 kb on - strand at 173.964 kb on + strand, within CA265_RS00730 at 174.068 kb on + strand, within CA265_RS00730 at 174.069 kb on - strand, within CA265_RS00730 at 174.080 kb on - strand, within CA265_RS00730 at 174.086 kb on - strand, within CA265_RS00730 at 174.086 kb on - strand, within CA265_RS00730 at 174.098 kb on + strand, within CA265_RS00730 at 174.098 kb on + strand, within CA265_RS00730 at 174.157 kb on - strand, within CA265_RS00730 at 174.174 kb on + strand, within CA265_RS00730 at 174.205 kb on + strand at 174.206 kb on - strand, within CA265_RS00730 at 174.234 kb on - strand, within CA265_RS00730 at 174.280 kb on + strand, within CA265_RS00730 at 174.281 kb on - strand, within CA265_RS00730 at 174.286 kb on - strand, within CA265_RS00730 at 174.344 kb on - strand, within CA265_RS00730 at 174.371 kb on + strand, within CA265_RS00730 at 174.372 kb on - strand, within CA265_RS00730 at 174.372 kb on - strand, within CA265_RS00730 at 174.413 kb on - strand, within CA265_RS00730 at 174.417 kb on + strand, within CA265_RS00730 at 174.496 kb on - strand, within CA265_RS00730 at 174.567 kb on + strand, within CA265_RS00730 at 174.568 kb on - strand, within CA265_RS00730 at 174.571 kb on + strand, within CA265_RS00730 at 174.576 kb on - strand, within CA265_RS00730
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 169,547 - CA265_RS00720 0.45 -0.7 169,583 + CA265_RS00720 0.47 -1.9 169,682 + CA265_RS00720 0.53 -0.7 169,682 + CA265_RS00720 0.53 -1.6 169,683 - CA265_RS00720 0.53 +0.0 169,689 - CA265_RS00720 0.54 -1.0 169,789 - CA265_RS00720 0.60 +1.2 169,789 - CA265_RS00720 0.60 -0.3 169,790 + CA265_RS00720 0.60 -0.6 169,790 + CA265_RS00720 0.60 -0.7 169,806 + CA265_RS00720 0.61 -0.2 169,873 + CA265_RS00720 0.65 -1.3 169,885 - CA265_RS00720 0.65 -0.3 169,918 + CA265_RS00720 0.67 -1.9 169,944 + CA265_RS00720 0.69 -1.4 169,944 + CA265_RS00720 0.69 -1.2 170,025 + CA265_RS00720 0.74 -0.6 170,265 - CA265_RS00720 0.88 +0.0 170,266 + CA265_RS00720 0.88 -0.7 170,266 + CA265_RS00720 0.88 +0.2 170,267 - CA265_RS00720 0.88 +0.2 170,267 - CA265_RS00720 0.88 +0.3 170,267 - CA265_RS00720 0.88 -0.3 170,267 - CA265_RS00720 0.88 -0.0 170,276 + CA265_RS00720 0.89 -0.7 170,352 + -1.7 170,353 - -0.2 170,401 - -0.2 170,401 - +0.6 170,503 - +0.2 170,503 - -1.1 170,510 - -0.6 170,523 + -0.0 170,532 + -0.2 170,532 + -0.1 170,584 + -1.8 170,585 - +0.4 170,596 - +0.8 170,626 + +1.1 170,646 + -0.0 170,719 + -0.1 170,725 + +0.6 170,726 - -0.2 170,778 + +1.0 170,778 + -0.1 170,790 + -1.6 170,800 + -0.1 170,927 - CA265_RS00725 0.13 +0.3 171,019 + CA265_RS00725 0.16 +0.7 171,023 + CA265_RS00725 0.16 +0.2 171,024 - CA265_RS00725 0.17 +0.1 171,024 - CA265_RS00725 0.17 +0.7 171,034 + CA265_RS00725 0.17 -0.1 171,046 - CA265_RS00725 0.17 +0.4 171,075 - CA265_RS00725 0.18 -0.2 171,075 - CA265_RS00725 0.18 +1.8 171,075 - CA265_RS00725 0.18 -0.7 171,160 + CA265_RS00725 0.21 +0.3 171,193 + CA265_RS00725 0.22 -1.8 171,340 + CA265_RS00725 0.27 -0.0 171,341 - CA265_RS00725 0.27 +0.1 171,445 + CA265_RS00725 0.30 +0.9 171,452 - CA265_RS00725 0.30 -0.1 171,452 - CA265_RS00725 0.30 +1.1 171,452 - CA265_RS00725 0.30 +0.5 171,482 - CA265_RS00725 0.31 +0.2 171,482 - CA265_RS00725 0.31 -0.3 171,516 - CA265_RS00725 0.32 +0.3 171,538 - CA265_RS00725 0.33 -0.5 171,538 - CA265_RS00725 0.33 -0.0 171,538 - CA265_RS00725 0.33 -1.1 171,582 - CA265_RS00725 0.34 +0.8 171,593 - CA265_RS00725 0.35 -0.5 171,634 - CA265_RS00725 0.36 +0.7 171,634 - CA265_RS00725 0.36 +0.1 171,638 - CA265_RS00725 0.36 -0.6 171,652 - CA265_RS00725 0.37 +0.1 171,707 + CA265_RS00725 0.38 +1.0 171,708 - CA265_RS00725 0.39 -0.4 171,710 + CA265_RS00725 0.39 +0.5 171,759 + CA265_RS00725 0.40 -0.6 171,806 + CA265_RS00725 0.42 +0.0 171,807 - CA265_RS00725 0.42 -1.0 171,820 - CA265_RS00725 0.42 -0.3 171,866 + CA265_RS00725 0.44 -0.7 171,896 - CA265_RS00725 0.45 +1.9 171,954 + CA265_RS00725 0.46 +1.2 171,954 + CA265_RS00725 0.46 -0.9 171,955 - CA265_RS00725 0.46 +3.7 171,955 - CA265_RS00725 0.46 -1.2 171,958 - CA265_RS00725 0.47 -0.1 171,961 - CA265_RS00725 0.47 +0.5 171,961 - CA265_RS00725 0.47 +0.6 171,990 + CA265_RS00725 0.48 -1.3 171,990 + CA265_RS00725 0.48 -0.1 171,991 - CA265_RS00725 0.48 -0.3 171,991 - CA265_RS00725 0.48 +1.0 171,991 - CA265_RS00725 0.48 +0.4 171,991 - CA265_RS00725 0.48 +0.7 172,011 + CA265_RS00725 0.48 +0.6 172,017 - CA265_RS00725 0.48 -0.9 172,019 + CA265_RS00725 0.49 +0.2 172,020 - CA265_RS00725 0.49 +0.2 172,056 + CA265_RS00725 0.50 -1.9 172,056 + CA265_RS00725 0.50 -0.2 172,071 - CA265_RS00725 0.50 +0.5 172,097 + CA265_RS00725 0.51 +0.2 172,105 + CA265_RS00725 0.51 -1.8 172,105 + CA265_RS00725 0.51 +0.3 172,106 - CA265_RS00725 0.51 +0.2 172,125 + CA265_RS00725 0.52 +0.3 172,125 + CA265_RS00725 0.52 -1.8 172,126 - CA265_RS00725 0.52 +0.8 172,130 - CA265_RS00725 0.52 +0.1 172,176 + CA265_RS00725 0.54 -0.3 172,193 + CA265_RS00725 0.54 +0.8 172,193 + CA265_RS00725 0.54 -0.1 172,223 - CA265_RS00725 0.55 +0.6 172,225 + CA265_RS00725 0.55 -0.1 172,225 + CA265_RS00725 0.55 +0.2 172,226 - CA265_RS00725 0.55 +0.5 172,292 + CA265_RS00725 0.57 +0.1 172,355 - CA265_RS00725 0.59 +0.2 172,433 - CA265_RS00725 0.62 +0.8 172,472 + CA265_RS00725 0.63 +0.5 172,473 - CA265_RS00725 0.63 +0.2 172,478 - CA265_RS00725 0.63 -1.2 172,478 - CA265_RS00725 0.63 +0.2 172,528 + CA265_RS00725 0.65 +0.7 172,529 - CA265_RS00725 0.65 +0.0 172,529 - CA265_RS00725 0.65 -0.4 172,567 + CA265_RS00725 0.66 -0.3 172,594 + CA265_RS00725 0.67 +0.1 172,615 - CA265_RS00725 0.68 +2.4 172,666 + CA265_RS00725 0.69 -0.8 172,792 + CA265_RS00725 0.73 -0.3 172,792 + CA265_RS00725 0.73 -0.9 172,820 + CA265_RS00725 0.74 +1.0 172,821 - CA265_RS00725 0.74 -1.5 172,933 + CA265_RS00725 0.78 -1.0 173,198 + CA265_RS00725 0.86 -1.8 173,207 - CA265_RS00725 0.87 +0.1 173,207 - CA265_RS00725 0.87 +1.6 173,207 - CA265_RS00725 0.87 +1.2 173,207 - CA265_RS00725 0.87 -1.2 173,296 + CA265_RS00725 0.90 -0.6 173,296 + CA265_RS00725 0.90 +0.3 173,297 - CA265_RS00725 0.90 -0.1 173,302 + CA265_RS00725 0.90 -1.2 173,387 + -2.6 173,387 + -3.1 173,390 + -4.0 173,390 + -0.4 173,473 - +0.4 173,530 - +0.0 173,563 - +0.6 173,594 - +0.3 173,611 + -1.3 173,611 + -4.1 173,673 - +0.1 173,683 + -0.1 173,756 + +0.4 173,791 + +0.3 173,813 + +0.5 173,832 - +0.6 173,846 + +1.3 173,848 + -0.8 173,848 + +1.6 173,848 + +0.6 173,849 - +0.5 173,849 - -0.2 173,849 - +0.9 173,849 - +0.8 173,877 + -0.2 173,878 - +0.4 173,964 + CA265_RS00730 0.14 +0.1 174,068 + CA265_RS00730 0.20 -0.0 174,069 - CA265_RS00730 0.20 +0.6 174,080 - CA265_RS00730 0.21 +0.6 174,086 - CA265_RS00730 0.21 +0.3 174,086 - CA265_RS00730 0.21 +0.4 174,098 + CA265_RS00730 0.22 -0.5 174,098 + CA265_RS00730 0.22 -0.1 174,157 - CA265_RS00730 0.25 +0.4 174,174 + CA265_RS00730 0.26 +0.3 174,205 + -0.2 174,206 - CA265_RS00730 0.28 -0.5 174,234 - CA265_RS00730 0.29 -1.0 174,280 + CA265_RS00730 0.32 +0.5 174,281 - CA265_RS00730 0.32 -1.4 174,286 - CA265_RS00730 0.32 +0.3 174,344 - CA265_RS00730 0.36 -0.6 174,371 + CA265_RS00730 0.37 -1.7 174,372 - CA265_RS00730 0.37 +0.2 174,372 - CA265_RS00730 0.37 -0.4 174,413 - CA265_RS00730 0.40 +0.6 174,417 + CA265_RS00730 0.40 -0.2 174,496 - CA265_RS00730 0.44 +0.4 174,567 + CA265_RS00730 0.48 -0.1 174,568 - CA265_RS00730 0.49 +0.0 174,571 + CA265_RS00730 0.49 +2.1 174,576 - CA265_RS00730 0.49 -0.5
Or see this region's nucleotide sequence