Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS00375 and CA265_RS00380 are separated by 11 nucleotides CA265_RS00380 and CA265_RS00385 are separated by 194 nucleotides CA265_RS00385 and CA265_RS00390 are separated by 30 nucleotides CA265_RS00390 and CA265_RS00395 are separated by 53 nucleotides CA265_RS00395 and CA265_RS00400 are separated by 45 nucleotides
CA265_RS00375: CA265_RS00375 - hypothetical protein, at 85,314 to 86,165
_RS00375
CA265_RS00380: CA265_RS00380 - hypothetical protein, at 86,177 to 86,464
_RS00380
CA265_RS00385: CA265_RS00385 - hypothetical protein, at 86,659 to 87,456
_RS00385
CA265_RS00390: CA265_RS00390 - hypothetical protein, at 87,487 to 87,993
_RS00390
CA265_RS00395: CA265_RS00395 - hypothetical protein, at 88,047 to 88,325
_RS00395
CA265_RS00400: CA265_RS00400 - hypothetical protein, at 88,371 to 90,113
_RS00400
Position (kb)
86
87
88 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 85.747 kb on - strand, within CA265_RS00375 at 85.764 kb on - strand, within CA265_RS00375 at 85.803 kb on - strand, within CA265_RS00375 at 85.858 kb on + strand, within CA265_RS00375 at 85.858 kb on + strand, within CA265_RS00375 at 85.858 kb on + strand, within CA265_RS00375 at 85.859 kb on - strand, within CA265_RS00375 at 85.900 kb on + strand, within CA265_RS00375 at 85.900 kb on + strand, within CA265_RS00375 at 85.911 kb on + strand, within CA265_RS00375 at 85.912 kb on - strand, within CA265_RS00375 at 86.010 kb on + strand, within CA265_RS00375 at 86.010 kb on + strand, within CA265_RS00375 at 86.014 kb on + strand, within CA265_RS00375 at 86.014 kb on + strand, within CA265_RS00375 at 86.015 kb on - strand, within CA265_RS00375 at 86.057 kb on + strand, within CA265_RS00375 at 86.061 kb on - strand, within CA265_RS00375 at 86.063 kb on + strand, within CA265_RS00375 at 86.064 kb on - strand, within CA265_RS00375 at 86.065 kb on + strand, within CA265_RS00375 at 86.066 kb on - strand, within CA265_RS00375 at 86.082 kb on - strand at 86.082 kb on - strand at 86.116 kb on - strand at 86.157 kb on + strand at 86.157 kb on + strand at 86.167 kb on + strand at 86.167 kb on + strand at 86.167 kb on + strand at 86.225 kb on + strand, within CA265_RS00380 at 86.226 kb on - strand, within CA265_RS00380 at 86.226 kb on - strand, within CA265_RS00380 at 86.230 kb on + strand, within CA265_RS00380 at 86.231 kb on - strand, within CA265_RS00380 at 86.390 kb on - strand, within CA265_RS00380 at 86.390 kb on - strand, within CA265_RS00380 at 86.523 kb on + strand at 86.523 kb on + strand at 86.524 kb on - strand at 86.524 kb on - strand at 86.524 kb on - strand at 86.654 kb on + strand at 86.654 kb on + strand at 86.659 kb on - strand at 86.854 kb on + strand, within CA265_RS00385 at 86.858 kb on + strand, within CA265_RS00385 at 86.858 kb on + strand, within CA265_RS00385 at 86.880 kb on - strand, within CA265_RS00385 at 86.914 kb on - strand, within CA265_RS00385 at 86.923 kb on - strand, within CA265_RS00385 at 86.979 kb on - strand, within CA265_RS00385 at 86.979 kb on - strand, within CA265_RS00385 at 86.997 kb on + strand, within CA265_RS00385 at 87.166 kb on + strand, within CA265_RS00385 at 87.173 kb on + strand, within CA265_RS00385 at 87.279 kb on + strand, within CA265_RS00385 at 87.280 kb on - strand, within CA265_RS00385 at 87.280 kb on - strand, within CA265_RS00385 at 87.306 kb on - strand, within CA265_RS00385 at 87.378 kb on + strand at 87.379 kb on - strand at 87.447 kb on - strand at 87.509 kb on + strand at 87.557 kb on + strand, within CA265_RS00390 at 87.613 kb on + strand, within CA265_RS00390 at 87.614 kb on - strand, within CA265_RS00390 at 87.618 kb on - strand, within CA265_RS00390 at 87.626 kb on - strand, within CA265_RS00390 at 87.629 kb on - strand, within CA265_RS00390 at 87.652 kb on + strand, within CA265_RS00390 at 87.652 kb on + strand, within CA265_RS00390 at 87.655 kb on + strand, within CA265_RS00390 at 87.655 kb on + strand, within CA265_RS00390 at 87.656 kb on - strand, within CA265_RS00390 at 87.676 kb on - strand, within CA265_RS00390 at 87.687 kb on + strand, within CA265_RS00390 at 87.729 kb on + strand, within CA265_RS00390 at 87.730 kb on - strand, within CA265_RS00390 at 87.748 kb on - strand, within CA265_RS00390 at 87.756 kb on + strand, within CA265_RS00390 at 87.769 kb on - strand, within CA265_RS00390 at 87.809 kb on + strand, within CA265_RS00390 at 87.852 kb on - strand, within CA265_RS00390 at 87.852 kb on - strand, within CA265_RS00390 at 87.892 kb on - strand, within CA265_RS00390 at 87.916 kb on - strand, within CA265_RS00390 at 87.940 kb on - strand, within CA265_RS00390 at 88.003 kb on + strand at 88.048 kb on + strand at 88.158 kb on - strand, within CA265_RS00395 at 88.173 kb on - strand, within CA265_RS00395 at 88.199 kb on + strand, within CA265_RS00395 at 88.227 kb on + strand, within CA265_RS00395 at 88.317 kb on + strand at 88.317 kb on + strand at 88.318 kb on - strand at 88.318 kb on - strand at 88.341 kb on - strand at 88.355 kb on - strand at 88.380 kb on - strand at 88.387 kb on + strand at 88.388 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 85,747 - CA265_RS00375 0.51 -0.5 85,764 - CA265_RS00375 0.53 +0.9 85,803 - CA265_RS00375 0.57 -0.2 85,858 + CA265_RS00375 0.64 -0.5 85,858 + CA265_RS00375 0.64 -0.1 85,858 + CA265_RS00375 0.64 +1.0 85,859 - CA265_RS00375 0.64 -0.2 85,900 + CA265_RS00375 0.69 -0.2 85,900 + CA265_RS00375 0.69 -0.2 85,911 + CA265_RS00375 0.70 -0.3 85,912 - CA265_RS00375 0.70 -0.7 86,010 + CA265_RS00375 0.82 -0.2 86,010 + CA265_RS00375 0.82 +0.0 86,014 + CA265_RS00375 0.82 -0.4 86,014 + CA265_RS00375 0.82 +0.7 86,015 - CA265_RS00375 0.82 +0.5 86,057 + CA265_RS00375 0.87 -0.6 86,061 - CA265_RS00375 0.88 -3.8 86,063 + CA265_RS00375 0.88 -0.2 86,064 - CA265_RS00375 0.88 -0.2 86,065 + CA265_RS00375 0.88 -0.2 86,066 - CA265_RS00375 0.88 +0.5 86,082 - -1.2 86,082 - -0.5 86,116 - +2.0 86,157 + +1.0 86,157 + +0.5 86,167 + +0.1 86,167 + -0.4 86,167 + -0.3 86,225 + CA265_RS00380 0.17 -0.3 86,226 - CA265_RS00380 0.17 -0.1 86,226 - CA265_RS00380 0.17 -0.9 86,230 + CA265_RS00380 0.18 -0.7 86,231 - CA265_RS00380 0.19 +0.1 86,390 - CA265_RS00380 0.74 -3.5 86,390 - CA265_RS00380 0.74 -4.0 86,523 + +0.8 86,523 + -0.2 86,524 - +0.1 86,524 - -0.2 86,524 - +0.2 86,654 + -1.4 86,654 + -0.1 86,659 - -0.2 86,854 + CA265_RS00385 0.24 -2.4 86,858 + CA265_RS00385 0.25 -0.6 86,858 + CA265_RS00385 0.25 +0.2 86,880 - CA265_RS00385 0.28 +0.4 86,914 - CA265_RS00385 0.32 -2.6 86,923 - CA265_RS00385 0.33 +0.3 86,979 - CA265_RS00385 0.40 -1.3 86,979 - CA265_RS00385 0.40 +0.3 86,997 + CA265_RS00385 0.42 +0.3 87,166 + CA265_RS00385 0.64 +0.0 87,173 + CA265_RS00385 0.64 -0.4 87,279 + CA265_RS00385 0.78 -0.7 87,280 - CA265_RS00385 0.78 -1.3 87,280 - CA265_RS00385 0.78 -0.6 87,306 - CA265_RS00385 0.81 -0.6 87,378 + +0.4 87,379 - -0.4 87,447 - -0.4 87,509 + +0.3 87,557 + CA265_RS00390 0.14 +0.6 87,613 + CA265_RS00390 0.25 +0.5 87,614 - CA265_RS00390 0.25 -0.2 87,618 - CA265_RS00390 0.26 -0.6 87,626 - CA265_RS00390 0.27 +0.3 87,629 - CA265_RS00390 0.28 -1.0 87,652 + CA265_RS00390 0.33 +2.9 87,652 + CA265_RS00390 0.33 +0.1 87,655 + CA265_RS00390 0.33 -0.5 87,655 + CA265_RS00390 0.33 +0.3 87,656 - CA265_RS00390 0.33 -0.3 87,676 - CA265_RS00390 0.37 -1.7 87,687 + CA265_RS00390 0.39 +1.1 87,729 + CA265_RS00390 0.48 +0.5 87,730 - CA265_RS00390 0.48 +0.2 87,748 - CA265_RS00390 0.51 -0.3 87,756 + CA265_RS00390 0.53 +1.0 87,769 - CA265_RS00390 0.56 -1.5 87,809 + CA265_RS00390 0.64 +0.1 87,852 - CA265_RS00390 0.72 -0.3 87,852 - CA265_RS00390 0.72 -0.4 87,892 - CA265_RS00390 0.80 +0.3 87,916 - CA265_RS00390 0.85 -0.7 87,940 - CA265_RS00390 0.89 +0.2 88,003 + +1.2 88,048 + -1.2 88,158 - CA265_RS00395 0.40 -0.9 88,173 - CA265_RS00395 0.45 +1.1 88,199 + CA265_RS00395 0.54 -0.5 88,227 + CA265_RS00395 0.65 -0.3 88,317 + -2.1 88,317 + -2.0 88,318 - +0.1 88,318 - -0.9 88,341 - -0.5 88,355 - -0.5 88,380 - -0.3 88,387 + -1.8 88,388 - +0.8
Or see this region's nucleotide sequence