Experiment: L-Tryptophan (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS12065 and BPHYT_RS12070 are separated by 73 nucleotides BPHYT_RS12070 and BPHYT_RS12075 overlap by 4 nucleotides BPHYT_RS12075 and BPHYT_RS12080 are separated by 3 nucleotides BPHYT_RS12080 and BPHYT_RS12085 are separated by 128 nucleotides
BPHYT_RS12065: BPHYT_RS12065 - RNA methyltransferase, at 2,750,697 to 2,751,482
_RS12065
BPHYT_RS12070: BPHYT_RS12070 - ribonuclease HII, at 2,751,556 to 2,752,302
_RS12070
BPHYT_RS12075: BPHYT_RS12075 - lipid-A-disaccharide synthase, at 2,752,299 to 2,753,468
_RS12075
BPHYT_RS12080: BPHYT_RS12080 - acyl-[acyl-carrier-protein]--UDP-N- acetylglucosamine O-acyltransferase, at 2,753,472 to 2,754,260
_RS12080
BPHYT_RS12085: BPHYT_RS12085 - 3-hydroxyacyl-ACP dehydratase, at 2,754,389 to 2,754,853
_RS12085
Position (kb)
2752
2753
2754 Strain fitness (log2 ratio)
-1
0
1 at 2751.404 kb on + strand at 2751.404 kb on + strand at 2751.404 kb on + strand at 2751.404 kb on + strand at 2751.448 kb on + strand at 2751.449 kb on - strand at 2751.535 kb on + strand at 2751.536 kb on - strand at 2752.040 kb on + strand, within BPHYT_RS12070 at 2752.040 kb on + strand, within BPHYT_RS12070 at 2752.100 kb on - strand, within BPHYT_RS12070 at 2752.276 kb on - strand at 2752.276 kb on - strand at 2754.391 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Tryptophan (C) remove 2,751,404 + -0.6 2,751,404 + -0.2 2,751,404 + -0.3 2,751,404 + -0.2 2,751,448 + -0.2 2,751,449 - -0.3 2,751,535 + -0.1 2,751,536 - -0.5 2,752,040 + BPHYT_RS12070 0.65 -0.4 2,752,040 + BPHYT_RS12070 0.65 -0.3 2,752,100 - BPHYT_RS12070 0.73 -0.3 2,752,276 - -1.5 2,752,276 - +0.5 2,754,391 - +1.0
Or see this region's nucleotide sequence