Experiment: L-Histidine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20214 and SM_b20215 are separated by 62 nucleotides SM_b20215 and SM_b20216 are separated by 140 nucleotides
SM_b20214: SM_b20214 - oxidoreductase, at 222,127 to 222,930
_b20214
SM_b20215: SM_b20215 - transcriptional regulator, at 222,993 to 223,973
_b20215
SM_b20216: SM_b20216 - epoxide hydrolase, at 224,114 to 225,160
_b20216
Position (kb) 222
223
224 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 222.034 kb on + strand at 222.091 kb on - strand at 222.191 kb on - strand at 222.276 kb on + strand, within SM_b20214 at 222.359 kb on + strand, within SM_b20214 at 222.360 kb on - strand, within SM_b20214 at 222.493 kb on + strand, within SM_b20214 at 222.494 kb on - strand, within SM_b20214 at 222.516 kb on - strand, within SM_b20214 at 222.544 kb on - strand, within SM_b20214 at 222.544 kb on - strand, within SM_b20214 at 222.544 kb on - strand, within SM_b20214 at 222.589 kb on + strand, within SM_b20214 at 222.666 kb on - strand, within SM_b20214 at 222.666 kb on - strand, within SM_b20214 at 222.850 kb on + strand at 222.850 kb on + strand at 222.850 kb on + strand at 222.850 kb on + strand at 222.850 kb on + strand at 222.851 kb on - strand at 222.928 kb on + strand at 222.928 kb on + strand at 222.941 kb on - strand at 223.032 kb on - strand at 223.032 kb on - strand at 223.084 kb on + strand at 223.129 kb on - strand, within SM_b20215 at 223.135 kb on + strand, within SM_b20215 at 223.136 kb on - strand, within SM_b20215 at 223.193 kb on + strand, within SM_b20215 at 223.194 kb on - strand, within SM_b20215 at 223.218 kb on + strand, within SM_b20215 at 223.430 kb on + strand, within SM_b20215 at 223.534 kb on - strand, within SM_b20215 at 223.666 kb on - strand, within SM_b20215 at 223.994 kb on + strand at 223.999 kb on + strand at 223.999 kb on + strand at 224.000 kb on - strand at 224.023 kb on + strand at 224.066 kb on + strand at 224.066 kb on + strand at 224.067 kb on - strand at 224.260 kb on + strand, within SM_b20216 at 224.260 kb on + strand, within SM_b20216 at 224.277 kb on + strand, within SM_b20216 at 224.307 kb on + strand, within SM_b20216 at 224.307 kb on + strand, within SM_b20216 at 224.307 kb on + strand, within SM_b20216 at 224.307 kb on + strand, within SM_b20216 at 224.308 kb on - strand, within SM_b20216 at 224.384 kb on + strand, within SM_b20216 at 224.385 kb on - strand, within SM_b20216 at 224.596 kb on + strand, within SM_b20216 at 224.651 kb on + strand, within SM_b20216 at 224.757 kb on - strand, within SM_b20216 at 224.933 kb on + strand, within SM_b20216
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (C) remove 222,034 + +0.4 222,091 - +2.2 222,191 - +0.1 222,276 + SM_b20214 0.19 +0.2 222,359 + SM_b20214 0.29 +0.2 222,360 - SM_b20214 0.29 -0.1 222,493 + SM_b20214 0.46 +0.3 222,494 - SM_b20214 0.46 -0.4 222,516 - SM_b20214 0.48 -0.2 222,544 - SM_b20214 0.52 +0.2 222,544 - SM_b20214 0.52 +0.1 222,544 - SM_b20214 0.52 -0.5 222,589 + SM_b20214 0.57 +0.2 222,666 - SM_b20214 0.67 +0.1 222,666 - SM_b20214 0.67 -1.0 222,850 + -0.2 222,850 + -0.1 222,850 + -1.0 222,850 + +0.2 222,850 + +0.7 222,851 - -0.1 222,928 + -0.3 222,928 + +0.0 222,941 - -0.2 223,032 - +0.2 223,032 - -2.9 223,084 + -0.3 223,129 - SM_b20215 0.14 -0.1 223,135 + SM_b20215 0.14 -0.1 223,136 - SM_b20215 0.15 +0.8 223,193 + SM_b20215 0.20 -0.2 223,194 - SM_b20215 0.20 +1.1 223,218 + SM_b20215 0.23 +0.5 223,430 + SM_b20215 0.45 +0.3 223,534 - SM_b20215 0.55 +0.7 223,666 - SM_b20215 0.69 +0.2 223,994 + +0.4 223,999 + +0.1 223,999 + -0.4 224,000 - -0.2 224,023 + +0.1 224,066 + -0.0 224,066 + -1.3 224,067 - +0.6 224,260 + SM_b20216 0.14 +0.3 224,260 + SM_b20216 0.14 +1.0 224,277 + SM_b20216 0.16 +0.5 224,307 + SM_b20216 0.18 -1.4 224,307 + SM_b20216 0.18 +0.7 224,307 + SM_b20216 0.18 +0.1 224,307 + SM_b20216 0.18 -0.2 224,308 - SM_b20216 0.19 -1.0 224,384 + SM_b20216 0.26 -0.4 224,385 - SM_b20216 0.26 -0.1 224,596 + SM_b20216 0.46 -0.2 224,651 + SM_b20216 0.51 +0.0 224,757 - SM_b20216 0.61 +0.4 224,933 + SM_b20216 0.78 +0.4
Or see this region's nucleotide sequence