Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS36540 and RR42_RS36545 are separated by 17 nucleotides RR42_RS36545 and RR42_RS36550 are separated by 4 nucleotides RR42_RS36550 and RR42_RS36555 are separated by 88 nucleotides
RR42_RS36540: RR42_RS36540 - CoA-transferase, at 3,653,261 to 3,654,484
_RS36540
RR42_RS36545: RR42_RS36545 - 3-alpha,7-alpha, 12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase, at 3,654,502 to 3,655,365
_RS36545
RR42_RS36550: RR42_RS36550 - 3-hydroxyacyl-CoA dehydrogenase, at 3,655,370 to 3,656,284
_RS36550
RR42_RS36555: RR42_RS36555 - MFS transporter, at 3,656,373 to 3,657,374
_RS36555
Position (kb)
3655
3656
3657 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3654.461 kb on + strand at 3654.508 kb on - strand at 3654.508 kb on - strand at 3654.691 kb on - strand, within RR42_RS36545 at 3654.720 kb on - strand, within RR42_RS36545 at 3655.223 kb on + strand, within RR42_RS36545 at 3655.223 kb on + strand, within RR42_RS36545 at 3655.308 kb on - strand at 3655.308 kb on - strand at 3655.791 kb on + strand, within RR42_RS36550 at 3655.792 kb on - strand, within RR42_RS36550 at 3656.064 kb on - strand, within RR42_RS36550 at 3656.118 kb on - strand, within RR42_RS36550 at 3656.259 kb on - strand at 3656.290 kb on + strand at 3656.291 kb on - strand at 3656.300 kb on + strand at 3656.435 kb on + strand at 3656.476 kb on + strand, within RR42_RS36555 at 3656.590 kb on + strand, within RR42_RS36555 at 3656.590 kb on + strand, within RR42_RS36555 at 3656.709 kb on - strand, within RR42_RS36555
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 3,654,461 + -0.5 3,654,508 - -1.8 3,654,508 - -0.5 3,654,691 - RR42_RS36545 0.22 +0.8 3,654,720 - RR42_RS36545 0.25 -1.7 3,655,223 + RR42_RS36545 0.83 -0.1 3,655,223 + RR42_RS36545 0.83 -0.7 3,655,308 - +0.8 3,655,308 - +1.1 3,655,791 + RR42_RS36550 0.46 -1.6 3,655,792 - RR42_RS36550 0.46 -3.2 3,656,064 - RR42_RS36550 0.76 -0.5 3,656,118 - RR42_RS36550 0.82 -1.0 3,656,259 - +0.1 3,656,290 + -1.3 3,656,291 - -0.1 3,656,300 + -0.1 3,656,435 + -0.1 3,656,476 + RR42_RS36555 0.10 -1.2 3,656,590 + RR42_RS36555 0.22 -0.1 3,656,590 + RR42_RS36555 0.22 -0.2 3,656,709 - RR42_RS36555 0.34 +0.3
Or see this region's nucleotide sequence