Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS36330 and RR42_RS36335 are separated by 185 nucleotides RR42_RS36335 and RR42_RS36340 are separated by 17 nucleotides RR42_RS36340 and RR42_RS36345 are separated by 208 nucleotides
RR42_RS36330: RR42_RS36330 - nitronate monooxygenase, at 3,603,087 to 3,604,061
_RS36330
RR42_RS36335: RR42_RS36335 - enoyl-CoA hydratase, at 3,604,247 to 3,605,134
_RS36335
RR42_RS36340: RR42_RS36340 - NADH dehydrogenase, at 3,605,152 to 3,605,823
_RS36340
RR42_RS36345: RR42_RS36345 - hydroxyproline-2-epimerase, at 3,606,032 to 3,607,000
_RS36345
Position (kb) 3604
3605
3606 Strain fitness (log2 ratio) -1
0
1
2 at 3603.256 kb on - strand, within RR42_RS36330 at 3603.256 kb on - strand, within RR42_RS36330 at 3603.818 kb on - strand, within RR42_RS36330 at 3603.989 kb on - strand at 3604.165 kb on + strand at 3604.165 kb on + strand at 3604.166 kb on - strand at 3604.166 kb on - strand at 3604.207 kb on + strand at 3604.207 kb on + strand at 3604.500 kb on - strand, within RR42_RS36335 at 3604.778 kb on + strand, within RR42_RS36335 at 3605.129 kb on - strand at 3605.380 kb on + strand, within RR42_RS36340 at 3605.381 kb on - strand, within RR42_RS36340 at 3605.381 kb on - strand, within RR42_RS36340 at 3605.389 kb on + strand, within RR42_RS36340 at 3605.596 kb on + strand, within RR42_RS36340 at 3605.596 kb on + strand, within RR42_RS36340 at 3605.596 kb on + strand, within RR42_RS36340 at 3605.596 kb on + strand, within RR42_RS36340 at 3605.597 kb on - strand, within RR42_RS36340 at 3605.597 kb on - strand, within RR42_RS36340 at 3605.860 kb on + strand at 3605.860 kb on + strand at 3605.928 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 3,603,256 - RR42_RS36330 0.17 +0.0 3,603,256 - RR42_RS36330 0.17 +0.3 3,603,818 - RR42_RS36330 0.75 +0.3 3,603,989 - +1.1 3,604,165 + +0.2 3,604,165 + +1.2 3,604,166 - -0.6 3,604,166 - -1.3 3,604,207 + +0.5 3,604,207 + -0.2 3,604,500 - RR42_RS36335 0.28 -0.9 3,604,778 + RR42_RS36335 0.60 -0.6 3,605,129 - -0.8 3,605,380 + RR42_RS36340 0.34 +1.8 3,605,381 - RR42_RS36340 0.34 +0.0 3,605,381 - RR42_RS36340 0.34 -1.2 3,605,389 + RR42_RS36340 0.35 -0.5 3,605,596 + RR42_RS36340 0.66 -0.7 3,605,596 + RR42_RS36340 0.66 +0.5 3,605,596 + RR42_RS36340 0.66 +0.1 3,605,596 + RR42_RS36340 0.66 +0.1 3,605,597 - RR42_RS36340 0.66 -0.4 3,605,597 - RR42_RS36340 0.66 -0.3 3,605,860 + -0.4 3,605,860 + -1.5 3,605,928 - -0.1
Or see this region's nucleotide sequence