Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS35995 and RR42_RS36000 are separated by 61 nucleotides RR42_RS36000 and RR42_RS36005 are separated by 82 nucleotides RR42_RS36005 and RR42_RS36010 are separated by 87 nucleotides
RR42_RS35995: RR42_RS35995 - membrane protein, at 3,523,646 to 3,525,043
_RS35995
RR42_RS36000: RR42_RS36000 - pilus assembly protein CpaB, at 3,525,105 to 3,526,076
_RS36000
RR42_RS36005: RR42_RS36005 - pilus assembly protein TadG, at 3,526,159 to 3,526,623
_RS36005
RR42_RS36010: RR42_RS36010 - peptidase, at 3,526,711 to 3,527,214
_RS36010
Position (kb)
3525
3526
3527 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3524.814 kb on - strand, within RR42_RS35995 at 3525.357 kb on + strand, within RR42_RS36000 at 3525.357 kb on + strand, within RR42_RS36000 at 3525.572 kb on - strand, within RR42_RS36000 at 3525.844 kb on + strand, within RR42_RS36000 at 3525.867 kb on + strand, within RR42_RS36000 at 3525.867 kb on + strand, within RR42_RS36000 at 3525.877 kb on - strand, within RR42_RS36000 at 3525.943 kb on + strand, within RR42_RS36000 at 3526.080 kb on - strand at 3526.080 kb on - strand at 3526.097 kb on - strand at 3526.225 kb on + strand, within RR42_RS36005 at 3526.225 kb on + strand, within RR42_RS36005 at 3526.225 kb on + strand, within RR42_RS36005 at 3526.225 kb on + strand, within RR42_RS36005 at 3526.226 kb on - strand, within RR42_RS36005 at 3526.226 kb on - strand, within RR42_RS36005 at 3526.226 kb on - strand, within RR42_RS36005 at 3526.514 kb on + strand, within RR42_RS36005 at 3526.623 kb on + strand at 3526.623 kb on + strand at 3526.624 kb on - strand at 3526.643 kb on + strand at 3526.712 kb on + strand at 3526.712 kb on + strand at 3526.755 kb on - strand at 3527.019 kb on - strand, within RR42_RS36010
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 3,524,814 - RR42_RS35995 0.84 +0.5 3,525,357 + RR42_RS36000 0.26 -0.7 3,525,357 + RR42_RS36000 0.26 -0.7 3,525,572 - RR42_RS36000 0.48 -0.8 3,525,844 + RR42_RS36000 0.76 -0.9 3,525,867 + RR42_RS36000 0.78 -1.5 3,525,867 + RR42_RS36000 0.78 -1.5 3,525,877 - RR42_RS36000 0.79 -0.1 3,525,943 + RR42_RS36000 0.86 -0.6 3,526,080 - -1.9 3,526,080 - -0.3 3,526,097 - -2.4 3,526,225 + RR42_RS36005 0.14 +0.1 3,526,225 + RR42_RS36005 0.14 +0.6 3,526,225 + RR42_RS36005 0.14 -0.4 3,526,225 + RR42_RS36005 0.14 -3.0 3,526,226 - RR42_RS36005 0.14 -0.2 3,526,226 - RR42_RS36005 0.14 +0.2 3,526,226 - RR42_RS36005 0.14 +2.1 3,526,514 + RR42_RS36005 0.76 +0.2 3,526,623 + +0.3 3,526,623 + -1.0 3,526,624 - +1.3 3,526,643 + -0.4 3,526,712 + -3.1 3,526,712 + +0.3 3,526,755 - -0.3 3,527,019 - RR42_RS36010 0.61 -0.2
Or see this region's nucleotide sequence