Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS34970 and RR42_RS34975 are separated by 49 nucleotides RR42_RS34975 and RR42_RS34980 are separated by 58 nucleotides RR42_RS34980 and RR42_RS34985 are separated by 52 nucleotides
RR42_RS34970: RR42_RS34970 - bifunctional D-altronate/D-mannonate dehydratase, at 3,296,791 to 3,298,002
_RS34970
RR42_RS34975: RR42_RS34975 - galactarate dehydratase, at 3,298,052 to 3,299,599
_RS34975
RR42_RS34980: RR42_RS34980 - oxidoreductase, at 3,299,658 to 3,300,413
_RS34980
RR42_RS34985: RR42_RS34985 - mandelate racemase, at 3,300,466 to 3,301,572
_RS34985
Position (kb)
3298
3299
3300 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3297.162 kb on - strand, within RR42_RS34970 at 3297.412 kb on + strand, within RR42_RS34970 at 3297.413 kb on - strand, within RR42_RS34970 at 3297.413 kb on - strand, within RR42_RS34970 at 3297.528 kb on - strand, within RR42_RS34970 at 3297.528 kb on - strand, within RR42_RS34970 at 3297.528 kb on - strand, within RR42_RS34970 at 3297.537 kb on + strand, within RR42_RS34970 at 3297.798 kb on - strand, within RR42_RS34970 at 3297.798 kb on - strand, within RR42_RS34970 at 3297.867 kb on - strand, within RR42_RS34970 at 3298.053 kb on + strand at 3298.053 kb on + strand at 3298.053 kb on + strand at 3298.053 kb on + strand at 3298.054 kb on - strand at 3298.054 kb on - strand at 3298.054 kb on - strand at 3298.098 kb on + strand at 3298.098 kb on + strand at 3298.099 kb on - strand at 3298.429 kb on - strand, within RR42_RS34975 at 3298.560 kb on + strand, within RR42_RS34975 at 3298.561 kb on - strand, within RR42_RS34975 at 3298.561 kb on - strand, within RR42_RS34975 at 3299.209 kb on + strand, within RR42_RS34975 at 3299.209 kb on + strand, within RR42_RS34975 at 3299.210 kb on - strand, within RR42_RS34975 at 3299.224 kb on - strand, within RR42_RS34975 at 3299.289 kb on + strand, within RR42_RS34975 at 3299.289 kb on + strand, within RR42_RS34975 at 3299.290 kb on - strand, within RR42_RS34975 at 3299.290 kb on - strand, within RR42_RS34975 at 3299.359 kb on + strand, within RR42_RS34975 at 3299.360 kb on - strand, within RR42_RS34975 at 3299.360 kb on - strand, within RR42_RS34975 at 3299.564 kb on + strand at 3299.565 kb on - strand at 3299.917 kb on + strand, within RR42_RS34980 at 3299.918 kb on - strand, within RR42_RS34980 at 3299.971 kb on + strand, within RR42_RS34980 at 3299.971 kb on + strand, within RR42_RS34980 at 3300.082 kb on + strand, within RR42_RS34980 at 3300.083 kb on - strand, within RR42_RS34980 at 3300.334 kb on + strand, within RR42_RS34980
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 3,297,162 - RR42_RS34970 0.31 -1.3 3,297,412 + RR42_RS34970 0.51 +0.6 3,297,413 - RR42_RS34970 0.51 -1.5 3,297,413 - RR42_RS34970 0.51 +0.0 3,297,528 - RR42_RS34970 0.61 +0.2 3,297,528 - RR42_RS34970 0.61 +0.5 3,297,528 - RR42_RS34970 0.61 +0.3 3,297,537 + RR42_RS34970 0.62 -1.5 3,297,798 - RR42_RS34970 0.83 +1.0 3,297,798 - RR42_RS34970 0.83 +0.5 3,297,867 - RR42_RS34970 0.89 +0.1 3,298,053 + +0.0 3,298,053 + +0.3 3,298,053 + +0.0 3,298,053 + +0.7 3,298,054 - -0.6 3,298,054 - +0.2 3,298,054 - +0.3 3,298,098 + +0.3 3,298,098 + +0.3 3,298,099 - -0.1 3,298,429 - RR42_RS34975 0.24 -1.3 3,298,560 + RR42_RS34975 0.33 -0.6 3,298,561 - RR42_RS34975 0.33 -0.9 3,298,561 - RR42_RS34975 0.33 -1.0 3,299,209 + RR42_RS34975 0.75 -0.2 3,299,209 + RR42_RS34975 0.75 +0.8 3,299,210 - RR42_RS34975 0.75 -0.2 3,299,224 - RR42_RS34975 0.76 -0.2 3,299,289 + RR42_RS34975 0.80 -0.3 3,299,289 + RR42_RS34975 0.80 -0.5 3,299,290 - RR42_RS34975 0.80 -0.9 3,299,290 - RR42_RS34975 0.80 -0.1 3,299,359 + RR42_RS34975 0.84 +0.2 3,299,360 - RR42_RS34975 0.84 +2.8 3,299,360 - RR42_RS34975 0.84 -0.1 3,299,564 + +1.2 3,299,565 - +0.3 3,299,917 + RR42_RS34980 0.34 +1.3 3,299,918 - RR42_RS34980 0.34 +0.1 3,299,971 + RR42_RS34980 0.41 +0.0 3,299,971 + RR42_RS34980 0.41 +1.0 3,300,082 + RR42_RS34980 0.56 -0.2 3,300,083 - RR42_RS34980 0.56 -1.3 3,300,334 + RR42_RS34980 0.89 +0.3
Or see this region's nucleotide sequence