Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS34895 and RR42_RS34900 are separated by 5 nucleotides RR42_RS34900 and RR42_RS34905 are separated by 1 nucleotides RR42_RS34905 and RR42_RS34910 are separated by 23 nucleotides RR42_RS34910 and RR42_RS34915 are separated by 65 nucleotides
RR42_RS34895: RR42_RS34895 - hypothetical protein, at 3,280,814 to 3,282,055
_RS34895
RR42_RS34900: RR42_RS34900 - hypothetical protein, at 3,282,061 to 3,282,726
_RS34900
RR42_RS34905: RR42_RS34905 - enoyl-CoA hydratase, at 3,282,728 to 3,283,456
_RS34905
RR42_RS34910: RR42_RS34910 - cysteine dioxygenase, at 3,283,480 to 3,284,082
_RS34910
RR42_RS34915: RR42_RS34915 - carnitine dehydratase, at 3,284,148 to 3,285,335
_RS34915
Position (kb)
3282
3283
3284 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3282.034 kb on - strand at 3282.073 kb on + strand at 3282.075 kb on + strand at 3282.075 kb on + strand at 3282.075 kb on + strand at 3282.075 kb on + strand at 3282.076 kb on - strand at 3282.082 kb on + strand at 3282.082 kb on + strand at 3283.347 kb on + strand, within RR42_RS34905 at 3283.348 kb on - strand, within RR42_RS34905 at 3283.348 kb on - strand, within RR42_RS34905 at 3283.466 kb on - strand at 3283.580 kb on + strand, within RR42_RS34910 at 3283.580 kb on + strand, within RR42_RS34910 at 3283.581 kb on - strand, within RR42_RS34910 at 3283.581 kb on - strand, within RR42_RS34910 at 3283.581 kb on - strand, within RR42_RS34910 at 3283.594 kb on + strand, within RR42_RS34910 at 3283.616 kb on + strand, within RR42_RS34910 at 3283.892 kb on + strand, within RR42_RS34910 at 3283.892 kb on + strand, within RR42_RS34910 at 3283.893 kb on - strand, within RR42_RS34910 at 3283.893 kb on - strand, within RR42_RS34910 at 3283.910 kb on + strand, within RR42_RS34910 at 3283.913 kb on + strand, within RR42_RS34910 at 3283.913 kb on + strand, within RR42_RS34910 at 3283.913 kb on + strand, within RR42_RS34910 at 3283.914 kb on - strand, within RR42_RS34910 at 3284.149 kb on + strand at 3284.150 kb on - strand at 3284.150 kb on - strand at 3284.150 kb on - strand at 3284.150 kb on - strand at 3284.150 kb on - strand at 3284.153 kb on + strand at 3284.154 kb on - strand at 3284.333 kb on + strand, within RR42_RS34915 at 3284.369 kb on - strand, within RR42_RS34915 at 3284.369 kb on - strand, within RR42_RS34915 at 3284.379 kb on - strand, within RR42_RS34915
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 3,282,034 - +0.5 3,282,073 + -1.5 3,282,075 + +1.1 3,282,075 + -0.7 3,282,075 + -0.0 3,282,075 + -1.5 3,282,076 - +0.6 3,282,082 + -0.4 3,282,082 + +3.3 3,283,347 + RR42_RS34905 0.85 +0.8 3,283,348 - RR42_RS34905 0.85 +0.8 3,283,348 - RR42_RS34905 0.85 +0.2 3,283,466 - -0.8 3,283,580 + RR42_RS34910 0.17 -0.1 3,283,580 + RR42_RS34910 0.17 -1.6 3,283,581 - RR42_RS34910 0.17 -0.3 3,283,581 - RR42_RS34910 0.17 -1.0 3,283,581 - RR42_RS34910 0.17 +0.3 3,283,594 + RR42_RS34910 0.19 +2.6 3,283,616 + RR42_RS34910 0.23 -0.7 3,283,892 + RR42_RS34910 0.68 -0.0 3,283,892 + RR42_RS34910 0.68 -0.5 3,283,893 - RR42_RS34910 0.68 +1.6 3,283,893 - RR42_RS34910 0.68 +1.2 3,283,910 + RR42_RS34910 0.71 -0.2 3,283,913 + RR42_RS34910 0.72 +0.6 3,283,913 + RR42_RS34910 0.72 +0.1 3,283,913 + RR42_RS34910 0.72 +0.3 3,283,914 - RR42_RS34910 0.72 +0.2 3,284,149 + +0.2 3,284,150 - -0.3 3,284,150 - -0.2 3,284,150 - +2.8 3,284,150 - +0.7 3,284,150 - -0.4 3,284,153 + +0.3 3,284,154 - -0.6 3,284,333 + RR42_RS34915 0.16 -0.9 3,284,369 - RR42_RS34915 0.19 +0.5 3,284,369 - RR42_RS34915 0.19 +0.4 3,284,379 - RR42_RS34915 0.19 +0.5
Or see this region's nucleotide sequence