Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS34210 and RR42_RS34215 are separated by 257 nucleotides RR42_RS34215 and RR42_RS34220 are separated by 278 nucleotides RR42_RS34220 and RR42_RS34225 are separated by 238 nucleotides
RR42_RS34210: RR42_RS34210 - hypothetical protein, at 3,100,101 to 3,100,490
_RS34210
RR42_RS34215: RR42_RS34215 - ferredoxin, at 3,100,748 to 3,101,068
_RS34215
RR42_RS34220: RR42_RS34220 - pyruvate dehydrogenase, at 3,101,347 to 3,104,022
_RS34220
RR42_RS34225: RR42_RS34225 - methylmalonate-semialdehyde dehydrogenase, at 3,104,261 to 3,105,781
_RS34225
Position (kb)
3101
3102
3103
3104
3105 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3100.496 kb on + strand at 3100.537 kb on - strand at 3100.582 kb on + strand at 3100.614 kb on - strand at 3100.730 kb on - strand at 3100.731 kb on + strand at 3100.731 kb on + strand at 3100.731 kb on + strand at 3100.893 kb on + strand, within RR42_RS34215 at 3101.308 kb on + strand at 3101.309 kb on - strand at 3101.547 kb on - strand at 3101.547 kb on - strand at 3101.852 kb on + strand, within RR42_RS34220 at 3101.853 kb on - strand, within RR42_RS34220 at 3101.906 kb on + strand, within RR42_RS34220 at 3101.907 kb on - strand, within RR42_RS34220 at 3101.907 kb on - strand, within RR42_RS34220 at 3101.930 kb on + strand, within RR42_RS34220 at 3101.931 kb on - strand, within RR42_RS34220 at 3101.931 kb on - strand, within RR42_RS34220 at 3101.933 kb on + strand, within RR42_RS34220 at 3101.933 kb on + strand, within RR42_RS34220 at 3102.194 kb on + strand, within RR42_RS34220 at 3102.197 kb on + strand, within RR42_RS34220 at 3102.202 kb on - strand, within RR42_RS34220 at 3102.202 kb on - strand, within RR42_RS34220 at 3102.203 kb on + strand, within RR42_RS34220 at 3102.203 kb on + strand, within RR42_RS34220 at 3102.203 kb on + strand, within RR42_RS34220 at 3102.203 kb on + strand, within RR42_RS34220 at 3102.204 kb on - strand, within RR42_RS34220 at 3102.204 kb on - strand, within RR42_RS34220 at 3102.204 kb on - strand, within RR42_RS34220 at 3102.204 kb on - strand, within RR42_RS34220 at 3102.204 kb on - strand, within RR42_RS34220 at 3102.236 kb on + strand, within RR42_RS34220 at 3102.236 kb on + strand, within RR42_RS34220 at 3102.237 kb on - strand, within RR42_RS34220 at 3102.237 kb on - strand, within RR42_RS34220 at 3102.323 kb on + strand, within RR42_RS34220 at 3102.324 kb on - strand, within RR42_RS34220 at 3102.324 kb on - strand, within RR42_RS34220 at 3102.326 kb on + strand, within RR42_RS34220 at 3102.327 kb on - strand, within RR42_RS34220 at 3102.357 kb on - strand, within RR42_RS34220 at 3102.357 kb on - strand, within RR42_RS34220 at 3102.378 kb on - strand, within RR42_RS34220 at 3102.674 kb on + strand, within RR42_RS34220 at 3102.674 kb on + strand, within RR42_RS34220 at 3102.674 kb on + strand, within RR42_RS34220 at 3102.674 kb on + strand, within RR42_RS34220 at 3102.750 kb on + strand, within RR42_RS34220 at 3102.882 kb on - strand, within RR42_RS34220 at 3102.924 kb on + strand, within RR42_RS34220 at 3102.995 kb on + strand, within RR42_RS34220 at 3103.025 kb on + strand, within RR42_RS34220 at 3103.230 kb on + strand, within RR42_RS34220 at 3103.230 kb on + strand, within RR42_RS34220 at 3103.231 kb on - strand, within RR42_RS34220 at 3103.382 kb on + strand, within RR42_RS34220 at 3103.383 kb on - strand, within RR42_RS34220 at 3103.383 kb on - strand, within RR42_RS34220 at 3103.461 kb on - strand, within RR42_RS34220 at 3103.496 kb on + strand, within RR42_RS34220 at 3103.661 kb on + strand, within RR42_RS34220 at 3103.661 kb on + strand, within RR42_RS34220 at 3103.661 kb on + strand, within RR42_RS34220 at 3103.662 kb on - strand, within RR42_RS34220 at 3103.662 kb on - strand, within RR42_RS34220 at 3103.662 kb on - strand, within RR42_RS34220 at 3103.662 kb on - strand, within RR42_RS34220 at 3103.662 kb on - strand, within RR42_RS34220 at 3103.796 kb on - strand at 3103.796 kb on - strand at 3103.823 kb on + strand at 3104.052 kb on - strand at 3104.052 kb on - strand at 3104.061 kb on + strand at 3104.121 kb on + strand at 3104.121 kb on + strand at 3104.199 kb on + strand at 3104.262 kb on + strand at 3104.262 kb on + strand at 3104.263 kb on - strand at 3104.273 kb on - strand at 3104.367 kb on + strand at 3104.368 kb on - strand at 3104.437 kb on + strand, within RR42_RS34225 at 3104.512 kb on - strand, within RR42_RS34225 at 3104.619 kb on + strand, within RR42_RS34225 at 3104.620 kb on - strand, within RR42_RS34225 at 3104.620 kb on - strand, within RR42_RS34225 at 3104.620 kb on - strand, within RR42_RS34225 at 3104.751 kb on + strand, within RR42_RS34225 at 3104.751 kb on + strand, within RR42_RS34225 at 3104.752 kb on - strand, within RR42_RS34225
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 3,100,496 + +0.5 3,100,537 - +0.2 3,100,582 + +0.1 3,100,614 - +1.2 3,100,730 - +0.5 3,100,731 + +0.3 3,100,731 + +0.7 3,100,731 + +0.2 3,100,893 + RR42_RS34215 0.45 -1.1 3,101,308 + +0.6 3,101,309 - +0.6 3,101,547 - -1.4 3,101,547 - +0.8 3,101,852 + RR42_RS34220 0.19 -0.2 3,101,853 - RR42_RS34220 0.19 +0.5 3,101,906 + RR42_RS34220 0.21 -2.4 3,101,907 - RR42_RS34220 0.21 -0.1 3,101,907 - RR42_RS34220 0.21 -1.5 3,101,930 + RR42_RS34220 0.22 +1.4 3,101,931 - RR42_RS34220 0.22 +1.0 3,101,931 - RR42_RS34220 0.22 -0.5 3,101,933 + RR42_RS34220 0.22 -0.0 3,101,933 + RR42_RS34220 0.22 -0.6 3,102,194 + RR42_RS34220 0.32 -1.1 3,102,197 + RR42_RS34220 0.32 -0.1 3,102,202 - RR42_RS34220 0.32 +0.5 3,102,202 - RR42_RS34220 0.32 -0.4 3,102,203 + RR42_RS34220 0.32 +0.0 3,102,203 + RR42_RS34220 0.32 +0.1 3,102,203 + RR42_RS34220 0.32 -0.1 3,102,203 + RR42_RS34220 0.32 -0.8 3,102,204 - RR42_RS34220 0.32 +0.2 3,102,204 - RR42_RS34220 0.32 +0.4 3,102,204 - RR42_RS34220 0.32 +0.3 3,102,204 - RR42_RS34220 0.32 -0.0 3,102,204 - RR42_RS34220 0.32 +0.5 3,102,236 + RR42_RS34220 0.33 -0.8 3,102,236 + RR42_RS34220 0.33 +0.4 3,102,237 - RR42_RS34220 0.33 +0.7 3,102,237 - RR42_RS34220 0.33 +0.5 3,102,323 + RR42_RS34220 0.36 +0.3 3,102,324 - RR42_RS34220 0.37 -0.2 3,102,324 - RR42_RS34220 0.37 -0.4 3,102,326 + RR42_RS34220 0.37 -0.9 3,102,327 - RR42_RS34220 0.37 -0.1 3,102,357 - RR42_RS34220 0.38 +0.2 3,102,357 - RR42_RS34220 0.38 +1.9 3,102,378 - RR42_RS34220 0.39 -0.6 3,102,674 + RR42_RS34220 0.50 +0.1 3,102,674 + RR42_RS34220 0.50 +0.5 3,102,674 + RR42_RS34220 0.50 +1.2 3,102,674 + RR42_RS34220 0.50 +0.5 3,102,750 + RR42_RS34220 0.52 +0.5 3,102,882 - RR42_RS34220 0.57 +1.7 3,102,924 + RR42_RS34220 0.59 -0.6 3,102,995 + RR42_RS34220 0.62 -0.2 3,103,025 + RR42_RS34220 0.63 +1.2 3,103,230 + RR42_RS34220 0.70 +0.0 3,103,230 + RR42_RS34220 0.70 +0.5 3,103,231 - RR42_RS34220 0.70 +1.0 3,103,382 + RR42_RS34220 0.76 -0.1 3,103,383 - RR42_RS34220 0.76 -0.3 3,103,383 - RR42_RS34220 0.76 +0.2 3,103,461 - RR42_RS34220 0.79 +1.4 3,103,496 + RR42_RS34220 0.80 -0.2 3,103,661 + RR42_RS34220 0.86 -0.4 3,103,661 + RR42_RS34220 0.86 -0.2 3,103,661 + RR42_RS34220 0.86 +0.2 3,103,662 - RR42_RS34220 0.87 -0.1 3,103,662 - RR42_RS34220 0.87 -0.8 3,103,662 - RR42_RS34220 0.87 -0.0 3,103,662 - RR42_RS34220 0.87 +1.1 3,103,662 - RR42_RS34220 0.87 -0.1 3,103,796 - -0.6 3,103,796 - +0.3 3,103,823 + +0.8 3,104,052 - -0.4 3,104,052 - +0.3 3,104,061 + +0.1 3,104,121 + -0.2 3,104,121 + -0.2 3,104,199 + -0.2 3,104,262 + -3.4 3,104,262 + +0.2 3,104,263 - +0.7 3,104,273 - -0.7 3,104,367 + -2.6 3,104,368 - -0.2 3,104,437 + RR42_RS34225 0.12 +0.1 3,104,512 - RR42_RS34225 0.17 +0.7 3,104,619 + RR42_RS34225 0.24 -0.7 3,104,620 - RR42_RS34225 0.24 +1.2 3,104,620 - RR42_RS34225 0.24 -1.4 3,104,620 - RR42_RS34225 0.24 -1.5 3,104,751 + RR42_RS34225 0.32 +0.6 3,104,751 + RR42_RS34225 0.32 +0.2 3,104,752 - RR42_RS34225 0.32 -0.1
Or see this region's nucleotide sequence