Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33805 and RR42_RS33810 are separated by 86 nucleotides RR42_RS33810 and RR42_RS33815 are separated by 55 nucleotides RR42_RS33815 and RR42_RS33820 are separated by 82 nucleotides
RR42_RS33805: RR42_RS33805 - 2-aminoadipate aminotransferase, at 3,013,944 to 3,015,458
_RS33805
RR42_RS33810: RR42_RS33810 - NIPSNAP family containing protein, at 3,015,545 to 3,015,850
_RS33810
RR42_RS33815: RR42_RS33815 - isochorismatase, at 3,015,906 to 3,016,460
_RS33815
RR42_RS33820: RR42_RS33820 - arabinose transporter permease, at 3,016,543 to 3,017,736
_RS33820
Position (kb)
3015
3016 Strain fitness (log2 ratio)
-1
0
1 at 3014.607 kb on + strand, within RR42_RS33805 at 3014.769 kb on + strand, within RR42_RS33805 at 3014.790 kb on + strand, within RR42_RS33805 at 3014.791 kb on - strand, within RR42_RS33805 at 3014.961 kb on + strand, within RR42_RS33805 at 3014.961 kb on + strand, within RR42_RS33805 at 3014.961 kb on + strand, within RR42_RS33805 at 3014.962 kb on - strand, within RR42_RS33805 at 3014.962 kb on - strand, within RR42_RS33805 at 3014.962 kb on - strand, within RR42_RS33805 at 3015.379 kb on - strand at 3015.546 kb on + strand at 3015.571 kb on + strand at 3015.572 kb on - strand at 3015.572 kb on - strand at 3015.579 kb on + strand, within RR42_RS33810 at 3015.580 kb on - strand, within RR42_RS33810 at 3015.580 kb on - strand, within RR42_RS33810 at 3015.658 kb on - strand, within RR42_RS33810 at 3015.658 kb on - strand, within RR42_RS33810 at 3015.666 kb on + strand, within RR42_RS33810 at 3015.735 kb on + strand, within RR42_RS33810 at 3015.736 kb on - strand, within RR42_RS33810 at 3015.736 kb on - strand, within RR42_RS33810 at 3015.736 kb on - strand, within RR42_RS33810 at 3015.736 kb on - strand, within RR42_RS33810 at 3015.826 kb on + strand at 3015.828 kb on + strand at 3015.828 kb on + strand at 3015.829 kb on - strand at 3015.829 kb on - strand at 3015.829 kb on - strand at 3015.829 kb on - strand at 3015.837 kb on + strand at 3016.193 kb on + strand, within RR42_RS33815 at 3016.431 kb on + strand at 3016.432 kb on - strand at 3016.432 kb on - strand at 3016.432 kb on - strand at 3016.432 kb on - strand at 3016.432 kb on - strand at 3016.432 kb on - strand at 3016.503 kb on + strand at 3016.504 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 3,014,607 + RR42_RS33805 0.44 +0.5 3,014,769 + RR42_RS33805 0.54 -0.9 3,014,790 + RR42_RS33805 0.56 -0.2 3,014,791 - RR42_RS33805 0.56 +0.1 3,014,961 + RR42_RS33805 0.67 +0.6 3,014,961 + RR42_RS33805 0.67 +0.9 3,014,961 + RR42_RS33805 0.67 -0.1 3,014,962 - RR42_RS33805 0.67 +0.2 3,014,962 - RR42_RS33805 0.67 -1.1 3,014,962 - RR42_RS33805 0.67 +0.4 3,015,379 - +0.4 3,015,546 + -0.5 3,015,571 + +0.8 3,015,572 - +0.9 3,015,572 - -1.7 3,015,579 + RR42_RS33810 0.11 -0.2 3,015,580 - RR42_RS33810 0.11 -1.1 3,015,580 - RR42_RS33810 0.11 +1.1 3,015,658 - RR42_RS33810 0.37 -1.2 3,015,658 - RR42_RS33810 0.37 +0.9 3,015,666 + RR42_RS33810 0.40 +0.3 3,015,735 + RR42_RS33810 0.62 -0.2 3,015,736 - RR42_RS33810 0.62 +0.0 3,015,736 - RR42_RS33810 0.62 -1.3 3,015,736 - RR42_RS33810 0.62 +1.3 3,015,736 - RR42_RS33810 0.62 +0.3 3,015,826 + -0.2 3,015,828 + +0.4 3,015,828 + +0.5 3,015,829 - -0.0 3,015,829 - +0.8 3,015,829 - +1.2 3,015,829 - +1.0 3,015,837 + -1.2 3,016,193 + RR42_RS33815 0.52 +1.1 3,016,431 + -0.1 3,016,432 - +0.2 3,016,432 - +0.4 3,016,432 - +0.3 3,016,432 - -1.7 3,016,432 - +1.2 3,016,432 - -0.5 3,016,503 + +0.0 3,016,504 - +0.6
Or see this region's nucleotide sequence