Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33250 and RR42_RS33255 are separated by 20 nucleotides RR42_RS33255 and RR42_RS33260 overlap by 4 nucleotides
RR42_RS33250: RR42_RS33250 - tyrosine protein kinase, at 2,874,255 to 2,876,510
_RS33250
RR42_RS33255: RR42_RS33255 - protein-tyrosine phosphatase, at 2,876,531 to 2,876,968
_RS33255
RR42_RS33260: RR42_RS33260 - multidrug MFS transporter, at 2,876,965 to 2,878,104
_RS33260
Position (kb)
2876
2877 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2875.693 kb on + strand, within RR42_RS33250 at 2875.959 kb on + strand, within RR42_RS33250 at 2876.080 kb on + strand, within RR42_RS33250 at 2876.177 kb on + strand, within RR42_RS33250 at 2876.177 kb on + strand, within RR42_RS33250 at 2876.178 kb on - strand, within RR42_RS33250 at 2876.179 kb on + strand, within RR42_RS33250 at 2876.179 kb on + strand at 2876.306 kb on + strand at 2876.306 kb on + strand at 2876.368 kb on + strand at 2876.384 kb on + strand at 2876.430 kb on + strand at 2876.443 kb on + strand at 2876.517 kb on + strand at 2876.699 kb on + strand, within RR42_RS33255 at 2876.700 kb on - strand, within RR42_RS33255 at 2876.858 kb on + strand, within RR42_RS33255 at 2877.062 kb on + strand at 2877.176 kb on + strand, within RR42_RS33260 at 2877.176 kb on + strand, within RR42_RS33260 at 2877.176 kb on + strand, within RR42_RS33260 at 2877.401 kb on - strand, within RR42_RS33260 at 2877.403 kb on + strand, within RR42_RS33260 at 2877.403 kb on + strand, within RR42_RS33260 at 2877.407 kb on + strand, within RR42_RS33260 at 2877.414 kb on + strand, within RR42_RS33260 at 2877.668 kb on - strand, within RR42_RS33260 at 2877.715 kb on + strand, within RR42_RS33260 at 2877.715 kb on + strand, within RR42_RS33260 at 2877.715 kb on + strand, within RR42_RS33260 at 2877.715 kb on + strand, within RR42_RS33260 at 2877.715 kb on + strand, within RR42_RS33260 at 2877.716 kb on - strand, within RR42_RS33260 at 2877.722 kb on + strand, within RR42_RS33260 at 2877.782 kb on + strand, within RR42_RS33260 at 2877.821 kb on - strand, within RR42_RS33260 at 2877.837 kb on + strand, within RR42_RS33260 at 2877.844 kb on + strand, within RR42_RS33260 at 2877.855 kb on + strand, within RR42_RS33260 at 2877.857 kb on + strand, within RR42_RS33260 at 2877.857 kb on + strand, within RR42_RS33260 at 2877.912 kb on + strand, within RR42_RS33260
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 2,875,693 + RR42_RS33250 0.64 +2.9 2,875,959 + RR42_RS33250 0.76 -0.7 2,876,080 + RR42_RS33250 0.81 -0.7 2,876,177 + RR42_RS33250 0.85 -2.0 2,876,177 + RR42_RS33250 0.85 -0.4 2,876,178 - RR42_RS33250 0.85 -1.2 2,876,179 + RR42_RS33250 0.85 -1.0 2,876,179 + +2.4 2,876,306 + +0.9 2,876,306 + -0.2 2,876,368 + -0.2 2,876,384 + -0.4 2,876,430 + +0.5 2,876,443 + +0.5 2,876,517 + -1.1 2,876,699 + RR42_RS33255 0.38 -0.4 2,876,700 - RR42_RS33255 0.39 -2.1 2,876,858 + RR42_RS33255 0.75 -1.5 2,877,062 + -1.2 2,877,176 + RR42_RS33260 0.19 -0.4 2,877,176 + RR42_RS33260 0.19 +1.5 2,877,176 + RR42_RS33260 0.19 +0.7 2,877,401 - RR42_RS33260 0.38 -0.1 2,877,403 + RR42_RS33260 0.38 -1.2 2,877,403 + RR42_RS33260 0.38 -0.2 2,877,407 + RR42_RS33260 0.39 +4.4 2,877,414 + RR42_RS33260 0.39 -1.8 2,877,668 - RR42_RS33260 0.62 -1.5 2,877,715 + RR42_RS33260 0.66 -0.6 2,877,715 + RR42_RS33260 0.66 -2.1 2,877,715 + RR42_RS33260 0.66 -3.5 2,877,715 + RR42_RS33260 0.66 -3.5 2,877,715 + RR42_RS33260 0.66 +1.1 2,877,716 - RR42_RS33260 0.66 -1.7 2,877,722 + RR42_RS33260 0.66 -2.6 2,877,782 + RR42_RS33260 0.72 -0.7 2,877,821 - RR42_RS33260 0.75 -1.7 2,877,837 + RR42_RS33260 0.76 -1.1 2,877,844 + RR42_RS33260 0.77 -1.1 2,877,855 + RR42_RS33260 0.78 +0.9 2,877,857 + RR42_RS33260 0.78 +0.4 2,877,857 + RR42_RS33260 0.78 +0.3 2,877,912 + RR42_RS33260 0.83 -0.6
Or see this region's nucleotide sequence