Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33245 and RR42_RS33250 are separated by 305 nucleotides RR42_RS33250 and RR42_RS33255 are separated by 20 nucleotides RR42_RS33255 and RR42_RS33260 overlap by 4 nucleotides
RR42_RS33245: RR42_RS33245 - mannose-1-phosphate guanyltransferase, at 2,872,525 to 2,873,949
_RS33245
RR42_RS33250: RR42_RS33250 - tyrosine protein kinase, at 2,874,255 to 2,876,510
_RS33250
RR42_RS33255: RR42_RS33255 - protein-tyrosine phosphatase, at 2,876,531 to 2,876,968
_RS33255
RR42_RS33260: RR42_RS33260 - multidrug MFS transporter, at 2,876,965 to 2,878,104
_RS33260
Position (kb)
2874
2875
2876
2877 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2873.291 kb on - strand, within RR42_RS33245 at 2873.320 kb on + strand, within RR42_RS33245 at 2873.324 kb on + strand, within RR42_RS33245 at 2873.325 kb on - strand, within RR42_RS33245 at 2873.325 kb on - strand, within RR42_RS33245 at 2873.325 kb on - strand, within RR42_RS33245 at 2873.366 kb on + strand, within RR42_RS33245 at 2873.367 kb on - strand, within RR42_RS33245 at 2873.367 kb on - strand, within RR42_RS33245 at 2873.385 kb on - strand, within RR42_RS33245 at 2873.471 kb on + strand, within RR42_RS33245 at 2873.587 kb on + strand, within RR42_RS33245 at 2873.588 kb on - strand, within RR42_RS33245 at 2873.673 kb on + strand, within RR42_RS33245 at 2873.918 kb on - strand at 2874.059 kb on - strand at 2874.223 kb on - strand at 2874.299 kb on - strand at 2874.304 kb on + strand at 2874.308 kb on - strand at 2874.308 kb on - strand at 2874.340 kb on + strand at 2874.465 kb on - strand at 2874.522 kb on + strand, within RR42_RS33250 at 2874.522 kb on + strand, within RR42_RS33250 at 2874.546 kb on + strand, within RR42_RS33250 at 2874.582 kb on + strand, within RR42_RS33250 at 2874.660 kb on + strand, within RR42_RS33250 at 2874.668 kb on + strand, within RR42_RS33250 at 2874.705 kb on + strand, within RR42_RS33250 at 2874.879 kb on - strand, within RR42_RS33250 at 2874.881 kb on + strand, within RR42_RS33250 at 2874.881 kb on + strand, within RR42_RS33250 at 2874.926 kb on + strand, within RR42_RS33250 at 2875.102 kb on + strand, within RR42_RS33250 at 2875.102 kb on + strand, within RR42_RS33250 at 2875.294 kb on + strand, within RR42_RS33250 at 2875.397 kb on + strand, within RR42_RS33250 at 2875.428 kb on + strand, within RR42_RS33250 at 2875.457 kb on + strand, within RR42_RS33250 at 2875.693 kb on + strand, within RR42_RS33250 at 2875.959 kb on + strand, within RR42_RS33250 at 2876.080 kb on + strand, within RR42_RS33250 at 2876.177 kb on + strand, within RR42_RS33250 at 2876.177 kb on + strand, within RR42_RS33250 at 2876.178 kb on - strand, within RR42_RS33250 at 2876.179 kb on + strand, within RR42_RS33250 at 2876.179 kb on + strand at 2876.306 kb on + strand at 2876.306 kb on + strand at 2876.368 kb on + strand at 2876.384 kb on + strand at 2876.430 kb on + strand at 2876.443 kb on + strand at 2876.517 kb on + strand at 2876.699 kb on + strand, within RR42_RS33255 at 2876.700 kb on - strand, within RR42_RS33255 at 2876.858 kb on + strand, within RR42_RS33255 at 2877.062 kb on + strand at 2877.176 kb on + strand, within RR42_RS33260 at 2877.176 kb on + strand, within RR42_RS33260 at 2877.176 kb on + strand, within RR42_RS33260 at 2877.401 kb on - strand, within RR42_RS33260 at 2877.403 kb on + strand, within RR42_RS33260 at 2877.403 kb on + strand, within RR42_RS33260 at 2877.407 kb on + strand, within RR42_RS33260 at 2877.414 kb on + strand, within RR42_RS33260
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 2,873,291 - RR42_RS33245 0.54 -2.1 2,873,320 + RR42_RS33245 0.56 -0.2 2,873,324 + RR42_RS33245 0.56 -1.7 2,873,325 - RR42_RS33245 0.56 +0.2 2,873,325 - RR42_RS33245 0.56 -0.3 2,873,325 - RR42_RS33245 0.56 +0.3 2,873,366 + RR42_RS33245 0.59 -1.0 2,873,367 - RR42_RS33245 0.59 -1.1 2,873,367 - RR42_RS33245 0.59 +0.1 2,873,385 - RR42_RS33245 0.60 +0.0 2,873,471 + RR42_RS33245 0.66 -0.1 2,873,587 + RR42_RS33245 0.75 +0.0 2,873,588 - RR42_RS33245 0.75 +0.2 2,873,673 + RR42_RS33245 0.81 -0.0 2,873,918 - +0.1 2,874,059 - -1.0 2,874,223 - +0.1 2,874,299 - -0.4 2,874,304 + -1.1 2,874,308 - +0.4 2,874,308 - -0.8 2,874,340 + -1.1 2,874,465 - -1.4 2,874,522 + RR42_RS33250 0.12 -0.7 2,874,522 + RR42_RS33250 0.12 -0.3 2,874,546 + RR42_RS33250 0.13 -1.7 2,874,582 + RR42_RS33250 0.14 -1.5 2,874,660 + RR42_RS33250 0.18 -1.3 2,874,668 + RR42_RS33250 0.18 +0.1 2,874,705 + RR42_RS33250 0.20 -1.5 2,874,879 - RR42_RS33250 0.28 -1.6 2,874,881 + RR42_RS33250 0.28 -2.3 2,874,881 + RR42_RS33250 0.28 +0.6 2,874,926 + RR42_RS33250 0.30 -0.7 2,875,102 + RR42_RS33250 0.38 -0.7 2,875,102 + RR42_RS33250 0.38 -0.7 2,875,294 + RR42_RS33250 0.46 -1.1 2,875,397 + RR42_RS33250 0.51 -2.7 2,875,428 + RR42_RS33250 0.52 +0.9 2,875,457 + RR42_RS33250 0.53 -0.4 2,875,693 + RR42_RS33250 0.64 +2.9 2,875,959 + RR42_RS33250 0.76 -0.7 2,876,080 + RR42_RS33250 0.81 -0.7 2,876,177 + RR42_RS33250 0.85 -2.0 2,876,177 + RR42_RS33250 0.85 -0.4 2,876,178 - RR42_RS33250 0.85 -1.2 2,876,179 + RR42_RS33250 0.85 -1.0 2,876,179 + +2.4 2,876,306 + +0.9 2,876,306 + -0.2 2,876,368 + -0.2 2,876,384 + -0.4 2,876,430 + +0.5 2,876,443 + +0.5 2,876,517 + -1.1 2,876,699 + RR42_RS33255 0.38 -0.4 2,876,700 - RR42_RS33255 0.39 -2.1 2,876,858 + RR42_RS33255 0.75 -1.5 2,877,062 + -1.2 2,877,176 + RR42_RS33260 0.19 -0.4 2,877,176 + RR42_RS33260 0.19 +1.5 2,877,176 + RR42_RS33260 0.19 +0.7 2,877,401 - RR42_RS33260 0.38 -0.1 2,877,403 + RR42_RS33260 0.38 -1.2 2,877,403 + RR42_RS33260 0.38 -0.2 2,877,407 + RR42_RS33260 0.39 +4.4 2,877,414 + RR42_RS33260 0.39 -1.8
Or see this region's nucleotide sequence