Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33050 and RR42_RS33055 are separated by 21 nucleotides RR42_RS33055 and RR42_RS33060 are separated by 35 nucleotides
RR42_RS33050: RR42_RS33050 - 2-oxoisovalerate dehydrogenase, at 2,820,964 to 2,821,941
_RS33050
RR42_RS33055: RR42_RS33055 - branched-chain alpha-keto acid dehydrogenase subunit E2, at 2,821,963 to 2,823,066
_RS33055
RR42_RS33060: RR42_RS33060 - twin-arginine translocation pathway signal, at 2,823,102 to 2,824,067
_RS33060
Position (kb)
2821
2822
2823
2824 Strain fitness (log2 ratio)
-2
-1
0
1 at 2821.222 kb on - strand, within RR42_RS33050 at 2821.222 kb on - strand, within RR42_RS33050 at 2821.222 kb on - strand, within RR42_RS33050 at 2821.222 kb on - strand, within RR42_RS33050 at 2821.403 kb on + strand, within RR42_RS33050 at 2821.403 kb on + strand, within RR42_RS33050 at 2821.404 kb on - strand, within RR42_RS33050 at 2821.491 kb on - strand, within RR42_RS33050 at 2821.493 kb on - strand, within RR42_RS33050 at 2821.514 kb on + strand, within RR42_RS33050 at 2821.514 kb on + strand, within RR42_RS33050 at 2821.515 kb on - strand, within RR42_RS33050 at 2821.545 kb on + strand, within RR42_RS33050 at 2821.704 kb on - strand, within RR42_RS33050 at 2821.811 kb on - strand, within RR42_RS33050 at 2821.879 kb on + strand at 2821.880 kb on - strand at 2821.880 kb on - strand at 2821.885 kb on + strand at 2821.886 kb on - strand at 2822.103 kb on - strand, within RR42_RS33055 at 2822.626 kb on + strand, within RR42_RS33055 at 2822.627 kb on - strand, within RR42_RS33055 at 2822.863 kb on + strand, within RR42_RS33055 at 2822.863 kb on + strand, within RR42_RS33055 at 2822.863 kb on + strand, within RR42_RS33055 at 2822.864 kb on - strand, within RR42_RS33055 at 2822.864 kb on - strand, within RR42_RS33055 at 2822.864 kb on - strand, within RR42_RS33055 at 2822.864 kb on - strand, within RR42_RS33055 at 2823.065 kb on - strand at 2823.065 kb on - strand at 2823.104 kb on - strand at 2823.206 kb on - strand, within RR42_RS33060 at 2823.317 kb on - strand, within RR42_RS33060 at 2823.325 kb on + strand, within RR42_RS33060 at 2823.325 kb on + strand, within RR42_RS33060 at 2823.326 kb on - strand, within RR42_RS33060 at 2823.433 kb on + strand, within RR42_RS33060 at 2823.462 kb on - strand, within RR42_RS33060 at 2823.496 kb on + strand, within RR42_RS33060 at 2823.517 kb on + strand, within RR42_RS33060 at 2823.517 kb on + strand, within RR42_RS33060 at 2823.518 kb on - strand, within RR42_RS33060 at 2823.518 kb on - strand, within RR42_RS33060 at 2823.743 kb on - strand, within RR42_RS33060 at 2823.806 kb on - strand, within RR42_RS33060 at 2823.806 kb on - strand, within RR42_RS33060 at 2823.878 kb on + strand, within RR42_RS33060 at 2823.879 kb on - strand, within RR42_RS33060
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 2,821,222 - RR42_RS33050 0.26 -0.2 2,821,222 - RR42_RS33050 0.26 +0.2 2,821,222 - RR42_RS33050 0.26 -0.5 2,821,222 - RR42_RS33050 0.26 +0.6 2,821,403 + RR42_RS33050 0.45 +1.3 2,821,403 + RR42_RS33050 0.45 +0.2 2,821,404 - RR42_RS33050 0.45 +0.3 2,821,491 - RR42_RS33050 0.54 +0.7 2,821,493 - RR42_RS33050 0.54 -0.2 2,821,514 + RR42_RS33050 0.56 +0.8 2,821,514 + RR42_RS33050 0.56 +0.0 2,821,515 - RR42_RS33050 0.56 +0.4 2,821,545 + RR42_RS33050 0.59 +0.2 2,821,704 - RR42_RS33050 0.76 +0.3 2,821,811 - RR42_RS33050 0.87 +0.7 2,821,879 + +1.3 2,821,880 - +0.0 2,821,880 - +0.3 2,821,885 + -0.0 2,821,886 - -0.3 2,822,103 - RR42_RS33055 0.13 +0.1 2,822,626 + RR42_RS33055 0.60 -0.7 2,822,627 - RR42_RS33055 0.60 +1.1 2,822,863 + RR42_RS33055 0.82 +0.4 2,822,863 + RR42_RS33055 0.82 +0.5 2,822,863 + RR42_RS33055 0.82 +0.7 2,822,864 - RR42_RS33055 0.82 +0.7 2,822,864 - RR42_RS33055 0.82 -0.0 2,822,864 - RR42_RS33055 0.82 +1.0 2,822,864 - RR42_RS33055 0.82 +0.8 2,823,065 - -0.3 2,823,065 - +1.1 2,823,104 - +0.3 2,823,206 - RR42_RS33060 0.11 -0.3 2,823,317 - RR42_RS33060 0.22 -2.3 2,823,325 + RR42_RS33060 0.23 +0.8 2,823,325 + RR42_RS33060 0.23 +0.4 2,823,326 - RR42_RS33060 0.23 +0.4 2,823,433 + RR42_RS33060 0.34 +0.6 2,823,462 - RR42_RS33060 0.37 +0.2 2,823,496 + RR42_RS33060 0.41 +1.6 2,823,517 + RR42_RS33060 0.43 -1.3 2,823,517 + RR42_RS33060 0.43 -1.7 2,823,518 - RR42_RS33060 0.43 +0.5 2,823,518 - RR42_RS33060 0.43 +1.3 2,823,743 - RR42_RS33060 0.66 -0.4 2,823,806 - RR42_RS33060 0.73 -1.5 2,823,806 - RR42_RS33060 0.73 -0.6 2,823,878 + RR42_RS33060 0.80 +0.3 2,823,879 - RR42_RS33060 0.80 -0.2
Or see this region's nucleotide sequence