Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33030 and RR42_RS33035 are separated by 79 nucleotides RR42_RS33035 and RR42_RS33040 are separated by 358 nucleotides RR42_RS33040 and RR42_RS33045 are separated by 133 nucleotides
RR42_RS33030: RR42_RS33030 - hypothetical protein, at 2,814,936 to 2,816,492
_RS33030
RR42_RS33035: RR42_RS33035 - type IV secretion protein Rhs, at 2,816,572 to 2,819,037
_RS33035
RR42_RS33040: RR42_RS33040 - hypothetical protein, at 2,819,396 to 2,819,716
_RS33040
RR42_RS33045: RR42_RS33045 - pyruvate dehydrogenase, at 2,819,850 to 2,820,914
_RS33045
Position (kb)
2816
2817
2818
2819
2820 Strain fitness (log2 ratio)
-2
-1
0
1 at 2815.714 kb on + strand, within RR42_RS33030 at 2815.721 kb on + strand, within RR42_RS33030 at 2815.722 kb on - strand, within RR42_RS33030 at 2815.795 kb on + strand, within RR42_RS33030 at 2815.795 kb on + strand, within RR42_RS33030 at 2815.798 kb on + strand, within RR42_RS33030 at 2815.798 kb on + strand, within RR42_RS33030 at 2815.843 kb on + strand, within RR42_RS33030 at 2815.843 kb on + strand, within RR42_RS33030 at 2815.843 kb on + strand, within RR42_RS33030 at 2815.844 kb on - strand, within RR42_RS33030 at 2815.853 kb on + strand, within RR42_RS33030 at 2815.853 kb on + strand, within RR42_RS33030 at 2815.853 kb on + strand, within RR42_RS33030 at 2815.854 kb on - strand, within RR42_RS33030 at 2815.854 kb on - strand, within RR42_RS33030 at 2815.854 kb on - strand, within RR42_RS33030 at 2815.854 kb on - strand, within RR42_RS33030 at 2815.855 kb on + strand, within RR42_RS33030 at 2815.855 kb on + strand, within RR42_RS33030 at 2815.874 kb on - strand, within RR42_RS33030 at 2815.874 kb on - strand, within RR42_RS33030 at 2815.942 kb on + strand, within RR42_RS33030 at 2815.943 kb on - strand, within RR42_RS33030 at 2815.947 kb on + strand, within RR42_RS33030 at 2815.948 kb on - strand, within RR42_RS33030 at 2815.969 kb on + strand, within RR42_RS33030 at 2815.970 kb on - strand, within RR42_RS33030 at 2815.974 kb on - strand, within RR42_RS33030 at 2816.005 kb on - strand, within RR42_RS33030 at 2816.005 kb on - strand, within RR42_RS33030 at 2816.106 kb on + strand, within RR42_RS33030 at 2816.119 kb on + strand, within RR42_RS33030 at 2816.120 kb on - strand, within RR42_RS33030 at 2816.206 kb on + strand, within RR42_RS33030 at 2816.206 kb on + strand, within RR42_RS33030 at 2816.206 kb on + strand, within RR42_RS33030 at 2816.367 kb on + strand at 2816.395 kb on - strand at 2816.457 kb on + strand at 2816.457 kb on + strand at 2816.457 kb on + strand at 2816.458 kb on - strand at 2816.458 kb on - strand at 2816.458 kb on - strand at 2816.459 kb on + strand at 2816.492 kb on + strand at 2816.739 kb on - strand at 2818.209 kb on - strand, within RR42_RS33035 at 2818.213 kb on - strand, within RR42_RS33035 at 2818.213 kb on - strand, within RR42_RS33035 at 2818.213 kb on - strand, within RR42_RS33035 at 2818.565 kb on + strand, within RR42_RS33035 at 2818.886 kb on + strand at 2818.886 kb on + strand at 2818.970 kb on + strand at 2818.970 kb on + strand at 2818.971 kb on - strand at 2819.010 kb on - strand at 2819.010 kb on - strand at 2819.105 kb on + strand at 2819.166 kb on + strand at 2819.166 kb on + strand at 2819.166 kb on + strand at 2819.187 kb on + strand at 2819.221 kb on - strand at 2819.363 kb on + strand at 2819.364 kb on - strand at 2819.474 kb on + strand, within RR42_RS33040 at 2819.474 kb on + strand, within RR42_RS33040 at 2819.474 kb on + strand, within RR42_RS33040 at 2819.513 kb on - strand, within RR42_RS33040 at 2819.846 kb on + strand at 2819.846 kb on + strand at 2819.964 kb on + strand, within RR42_RS33045 at 2819.964 kb on + strand, within RR42_RS33045
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 2,815,714 + RR42_RS33030 0.50 +0.4 2,815,721 + RR42_RS33030 0.50 -0.3 2,815,722 - RR42_RS33030 0.50 -0.1 2,815,795 + RR42_RS33030 0.55 +0.4 2,815,795 + RR42_RS33030 0.55 -0.9 2,815,798 + RR42_RS33030 0.55 +0.2 2,815,798 + RR42_RS33030 0.55 -0.7 2,815,843 + RR42_RS33030 0.58 -0.3 2,815,843 + RR42_RS33030 0.58 -2.1 2,815,843 + RR42_RS33030 0.58 +0.3 2,815,844 - RR42_RS33030 0.58 +0.1 2,815,853 + RR42_RS33030 0.59 -0.6 2,815,853 + RR42_RS33030 0.59 -0.1 2,815,853 + RR42_RS33030 0.59 -0.4 2,815,854 - RR42_RS33030 0.59 +1.0 2,815,854 - RR42_RS33030 0.59 -0.2 2,815,854 - RR42_RS33030 0.59 +1.6 2,815,854 - RR42_RS33030 0.59 -0.2 2,815,855 + RR42_RS33030 0.59 +0.1 2,815,855 + RR42_RS33030 0.59 -2.3 2,815,874 - RR42_RS33030 0.60 -0.1 2,815,874 - RR42_RS33030 0.60 +0.8 2,815,942 + RR42_RS33030 0.65 -0.8 2,815,943 - RR42_RS33030 0.65 -0.7 2,815,947 + RR42_RS33030 0.65 -2.2 2,815,948 - RR42_RS33030 0.65 -0.6 2,815,969 + RR42_RS33030 0.66 -0.7 2,815,970 - RR42_RS33030 0.66 +0.1 2,815,974 - RR42_RS33030 0.67 -0.1 2,816,005 - RR42_RS33030 0.69 -1.7 2,816,005 - RR42_RS33030 0.69 -0.4 2,816,106 + RR42_RS33030 0.75 +0.6 2,816,119 + RR42_RS33030 0.76 -0.8 2,816,120 - RR42_RS33030 0.76 -0.0 2,816,206 + RR42_RS33030 0.82 +0.7 2,816,206 + RR42_RS33030 0.82 -1.7 2,816,206 + RR42_RS33030 0.82 -0.6 2,816,367 + -1.1 2,816,395 - -0.5 2,816,457 + -0.4 2,816,457 + +0.1 2,816,457 + -1.3 2,816,458 - -0.7 2,816,458 - +0.8 2,816,458 - -1.5 2,816,459 + +0.6 2,816,492 + -1.0 2,816,739 - -2.1 2,818,209 - RR42_RS33035 0.66 -0.2 2,818,213 - RR42_RS33035 0.67 -1.1 2,818,213 - RR42_RS33035 0.67 +0.2 2,818,213 - RR42_RS33035 0.67 +0.0 2,818,565 + RR42_RS33035 0.81 -1.0 2,818,886 + -0.3 2,818,886 + +0.2 2,818,970 + -0.7 2,818,970 + -0.2 2,818,971 - +0.3 2,819,010 - -1.1 2,819,010 - -0.1 2,819,105 + +0.7 2,819,166 + -0.4 2,819,166 + +0.0 2,819,166 + +1.2 2,819,187 + -0.5 2,819,221 - +0.6 2,819,363 + +1.6 2,819,364 - -0.7 2,819,474 + RR42_RS33040 0.24 -0.4 2,819,474 + RR42_RS33040 0.24 -0.0 2,819,474 + RR42_RS33040 0.24 +0.2 2,819,513 - RR42_RS33040 0.36 +0.1 2,819,846 + +0.5 2,819,846 + +0.4 2,819,964 + RR42_RS33045 0.11 +0.3 2,819,964 + RR42_RS33045 0.11 +0.2
Or see this region's nucleotide sequence