Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32925 and RR42_RS32930 are separated by 120 nucleotides RR42_RS32930 and RR42_RS32935 overlap by 7 nucleotides RR42_RS32935 and RR42_RS32940 are separated by 279 nucleotides
RR42_RS32925: RR42_RS32925 - alpha/beta hydrolase, at 2,786,779 to 2,787,735
_RS32925
RR42_RS32930: RR42_RS32930 - twitching motility protein PilT, at 2,787,856 to 2,788,224
_RS32930
RR42_RS32935: RR42_RS32935 - CopG family transcriptional regulator, at 2,788,218 to 2,788,436
_RS32935
RR42_RS32940: RR42_RS32940 - sulfite reductase, at 2,788,716 to 2,790,446
_RS32940
Position (kb)
2788
2789 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2787.268 kb on + strand, within RR42_RS32925 at 2787.268 kb on + strand, within RR42_RS32925 at 2787.268 kb on + strand, within RR42_RS32925 at 2787.287 kb on - strand, within RR42_RS32925 at 2787.289 kb on - strand, within RR42_RS32925 at 2787.463 kb on + strand, within RR42_RS32925 at 2787.464 kb on - strand, within RR42_RS32925 at 2787.464 kb on - strand, within RR42_RS32925 at 2787.469 kb on + strand, within RR42_RS32925 at 2787.470 kb on - strand, within RR42_RS32925 at 2787.496 kb on + strand, within RR42_RS32925 at 2787.496 kb on + strand, within RR42_RS32925 at 2787.497 kb on - strand, within RR42_RS32925 at 2787.497 kb on - strand, within RR42_RS32925 at 2787.505 kb on + strand, within RR42_RS32925 at 2787.506 kb on - strand, within RR42_RS32925 at 2787.516 kb on + strand, within RR42_RS32925 at 2787.621 kb on + strand, within RR42_RS32925 at 2787.869 kb on + strand at 2787.870 kb on - strand at 2787.913 kb on - strand, within RR42_RS32930 at 2787.913 kb on - strand, within RR42_RS32930 at 2787.913 kb on - strand, within RR42_RS32930 at 2787.960 kb on + strand, within RR42_RS32930 at 2788.197 kb on + strand at 2788.198 kb on - strand at 2788.324 kb on + strand, within RR42_RS32935 at 2788.324 kb on + strand, within RR42_RS32935 at 2788.324 kb on + strand, within RR42_RS32935 at 2788.324 kb on + strand, within RR42_RS32935 at 2788.325 kb on - strand, within RR42_RS32935 at 2788.325 kb on - strand, within RR42_RS32935 at 2788.325 kb on - strand, within RR42_RS32935 at 2788.350 kb on + strand, within RR42_RS32935 at 2788.350 kb on + strand, within RR42_RS32935 at 2788.461 kb on + strand at 2788.654 kb on + strand at 2788.654 kb on + strand at 2788.719 kb on + strand at 2788.719 kb on + strand at 2788.719 kb on + strand at 2788.719 kb on + strand at 2788.719 kb on + strand at 2788.719 kb on + strand at 2788.719 kb on + strand at 2788.719 kb on + strand at 2788.719 kb on + strand at 2788.719 kb on + strand at 2788.719 kb on + strand at 2788.719 kb on + strand at 2788.719 kb on + strand at 2788.719 kb on + strand at 2788.719 kb on + strand at 2788.720 kb on - strand at 2788.720 kb on - strand at 2788.720 kb on - strand at 2788.720 kb on - strand at 2788.720 kb on - strand at 2788.720 kb on - strand at 2788.720 kb on - strand at 2788.720 kb on - strand at 2788.720 kb on - strand at 2788.720 kb on - strand at 2788.720 kb on - strand at 2788.720 kb on - strand at 2788.720 kb on - strand at 2788.725 kb on + strand at 2788.725 kb on + strand at 2788.725 kb on + strand at 2788.725 kb on + strand at 2788.726 kb on - strand at 2788.726 kb on - strand at 2788.851 kb on + strand at 2788.851 kb on + strand at 2788.852 kb on - strand at 2788.852 kb on - strand at 2788.852 kb on - strand at 2788.852 kb on - strand at 2788.852 kb on - strand at 2788.894 kb on - strand, within RR42_RS32940 at 2788.937 kb on - strand, within RR42_RS32940 at 2789.397 kb on + strand, within RR42_RS32940 at 2789.397 kb on + strand, within RR42_RS32940 at 2789.422 kb on - strand, within RR42_RS32940
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 2,787,268 + RR42_RS32925 0.51 -0.4 2,787,268 + RR42_RS32925 0.51 +0.0 2,787,268 + RR42_RS32925 0.51 -0.2 2,787,287 - RR42_RS32925 0.53 -0.7 2,787,289 - RR42_RS32925 0.53 -0.2 2,787,463 + RR42_RS32925 0.71 +0.5 2,787,464 - RR42_RS32925 0.72 +2.4 2,787,464 - RR42_RS32925 0.72 +0.3 2,787,469 + RR42_RS32925 0.72 +0.8 2,787,470 - RR42_RS32925 0.72 +0.4 2,787,496 + RR42_RS32925 0.75 +0.5 2,787,496 + RR42_RS32925 0.75 +0.3 2,787,497 - RR42_RS32925 0.75 +0.4 2,787,497 - RR42_RS32925 0.75 +0.8 2,787,505 + RR42_RS32925 0.76 +1.0 2,787,506 - RR42_RS32925 0.76 +0.8 2,787,516 + RR42_RS32925 0.77 -0.3 2,787,621 + RR42_RS32925 0.88 -0.0 2,787,869 + +1.0 2,787,870 - +0.3 2,787,913 - RR42_RS32930 0.15 +0.2 2,787,913 - RR42_RS32930 0.15 +0.1 2,787,913 - RR42_RS32930 0.15 -1.4 2,787,960 + RR42_RS32930 0.28 -0.7 2,788,197 + -1.0 2,788,198 - +0.1 2,788,324 + RR42_RS32935 0.48 +0.9 2,788,324 + RR42_RS32935 0.48 +1.8 2,788,324 + RR42_RS32935 0.48 +0.7 2,788,324 + RR42_RS32935 0.48 -1.7 2,788,325 - RR42_RS32935 0.49 -0.3 2,788,325 - RR42_RS32935 0.49 +2.7 2,788,325 - RR42_RS32935 0.49 -0.3 2,788,350 + RR42_RS32935 0.60 +0.6 2,788,350 + RR42_RS32935 0.60 -0.0 2,788,461 + -0.7 2,788,654 + -0.7 2,788,654 + -0.3 2,788,719 + -1.2 2,788,719 + +1.8 2,788,719 + +0.2 2,788,719 + -0.1 2,788,719 + +0.2 2,788,719 + -0.6 2,788,719 + +4.0 2,788,719 + +0.6 2,788,719 + -0.0 2,788,719 + +0.4 2,788,719 + -1.2 2,788,719 + +0.1 2,788,719 + -0.1 2,788,719 + +1.4 2,788,719 + -0.6 2,788,720 - +0.5 2,788,720 - -0.5 2,788,720 - -0.5 2,788,720 - +0.2 2,788,720 - +0.2 2,788,720 - -1.1 2,788,720 - +0.1 2,788,720 - -0.4 2,788,720 - +0.0 2,788,720 - +0.1 2,788,720 - +1.0 2,788,720 - -0.8 2,788,720 - -0.4 2,788,725 + +0.0 2,788,725 + -0.6 2,788,725 + -0.9 2,788,725 + -2.4 2,788,726 - -1.2 2,788,726 - +0.8 2,788,851 + +0.0 2,788,851 + +0.3 2,788,852 - +0.1 2,788,852 - +0.7 2,788,852 - -0.5 2,788,852 - -0.2 2,788,852 - -0.7 2,788,894 - RR42_RS32940 0.10 -0.1 2,788,937 - RR42_RS32940 0.13 -0.5 2,789,397 + RR42_RS32940 0.39 +0.7 2,789,397 + RR42_RS32940 0.39 +1.5 2,789,422 - RR42_RS32940 0.41 +0.2
Or see this region's nucleotide sequence