Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32595 and RR42_RS32600 are separated by 8 nucleotides RR42_RS32600 and RR42_RS32605 are separated by 680 nucleotides
RR42_RS32595: RR42_RS32595 - cytochrome o ubiquinol oxidase subunit I, at 2,707,794 to 2,709,770
_RS32595
RR42_RS32600: RR42_RS32600 - cytochrome O ubiquinol oxidase subunit II, at 2,709,779 to 2,710,747
_RS32600
RR42_RS32605: RR42_RS32605 - sulfonate ABC transporter substrate-binding protein, at 2,711,428 to 2,712,396
_RS32605
Position (kb)
2709
2710
2711 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2708.833 kb on + strand, within RR42_RS32595 at 2708.909 kb on - strand, within RR42_RS32595 at 2708.947 kb on + strand, within RR42_RS32595 at 2708.947 kb on + strand, within RR42_RS32595 at 2708.948 kb on - strand, within RR42_RS32595 at 2709.203 kb on - strand, within RR42_RS32595 at 2709.229 kb on + strand, within RR42_RS32595 at 2709.475 kb on + strand, within RR42_RS32595 at 2709.589 kb on + strand at 2709.590 kb on - strand at 2709.662 kb on - strand at 2709.775 kb on - strand at 2709.915 kb on + strand, within RR42_RS32600 at 2709.916 kb on - strand, within RR42_RS32600 at 2709.937 kb on - strand, within RR42_RS32600 at 2710.200 kb on + strand, within RR42_RS32600 at 2710.200 kb on + strand, within RR42_RS32600 at 2710.201 kb on - strand, within RR42_RS32600 at 2710.739 kb on + strand at 2711.317 kb on + strand at 2711.331 kb on - strand at 2711.382 kb on - strand at 2711.382 kb on - strand at 2711.740 kb on + strand, within RR42_RS32605 at 2711.740 kb on + strand, within RR42_RS32605 at 2711.740 kb on + strand, within RR42_RS32605
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 2,708,833 + RR42_RS32595 0.53 -0.7 2,708,909 - RR42_RS32595 0.56 -0.7 2,708,947 + RR42_RS32595 0.58 -0.2 2,708,947 + RR42_RS32595 0.58 -0.3 2,708,948 - RR42_RS32595 0.58 -0.2 2,709,203 - RR42_RS32595 0.71 -0.8 2,709,229 + RR42_RS32595 0.73 -2.5 2,709,475 + RR42_RS32595 0.85 -0.6 2,709,589 + +2.1 2,709,590 - -1.3 2,709,662 - -2.5 2,709,775 - -1.2 2,709,915 + RR42_RS32600 0.14 -0.4 2,709,916 - RR42_RS32600 0.14 -0.2 2,709,937 - RR42_RS32600 0.16 -1.2 2,710,200 + RR42_RS32600 0.43 -1.7 2,710,200 + RR42_RS32600 0.43 +0.8 2,710,201 - RR42_RS32600 0.44 -0.5 2,710,739 + -2.0 2,711,317 + -0.4 2,711,331 - -0.9 2,711,382 - -0.6 2,711,382 - -0.2 2,711,740 + RR42_RS32605 0.32 -0.6 2,711,740 + RR42_RS32605 0.32 -0.3 2,711,740 + RR42_RS32605 0.32 -0.8
Or see this region's nucleotide sequence