Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32585 and RR42_RS32590 overlap by 1 nucleotides RR42_RS32590 and RR42_RS32595 are separated by 3 nucleotides RR42_RS32595 and RR42_RS32600 are separated by 8 nucleotides
RR42_RS32585: RR42_RS32585 - cytochrome C oxidase, at 2,706,775 to 2,707,140
_RS32585
RR42_RS32590: RR42_RS32590 - cytochrome o ubiquinol oxidase subunit III, at 2,707,140 to 2,707,790
_RS32590
RR42_RS32595: RR42_RS32595 - cytochrome o ubiquinol oxidase subunit I, at 2,707,794 to 2,709,770
_RS32595
RR42_RS32600: RR42_RS32600 - cytochrome O ubiquinol oxidase subunit II, at 2,709,779 to 2,710,747
_RS32600
Position (kb)
2707
2708
2709
2710 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2707.052 kb on + strand, within RR42_RS32585 at 2707.053 kb on - strand, within RR42_RS32585 at 2707.131 kb on + strand at 2707.131 kb on + strand at 2707.132 kb on - strand at 2707.553 kb on - strand, within RR42_RS32590 at 2707.773 kb on + strand at 2707.774 kb on - strand at 2707.861 kb on + strand at 2707.861 kb on + strand at 2707.943 kb on + strand at 2707.991 kb on - strand at 2708.122 kb on + strand, within RR42_RS32595 at 2708.290 kb on + strand, within RR42_RS32595 at 2708.462 kb on + strand, within RR42_RS32595 at 2708.462 kb on + strand, within RR42_RS32595 at 2708.659 kb on + strand, within RR42_RS32595 at 2708.833 kb on + strand, within RR42_RS32595 at 2708.909 kb on - strand, within RR42_RS32595 at 2708.947 kb on + strand, within RR42_RS32595 at 2708.947 kb on + strand, within RR42_RS32595 at 2708.948 kb on - strand, within RR42_RS32595 at 2709.203 kb on - strand, within RR42_RS32595 at 2709.229 kb on + strand, within RR42_RS32595 at 2709.475 kb on + strand, within RR42_RS32595 at 2709.589 kb on + strand at 2709.590 kb on - strand at 2709.662 kb on - strand at 2709.775 kb on - strand at 2709.915 kb on + strand, within RR42_RS32600 at 2709.916 kb on - strand, within RR42_RS32600 at 2709.937 kb on - strand, within RR42_RS32600 at 2710.200 kb on + strand, within RR42_RS32600 at 2710.200 kb on + strand, within RR42_RS32600 at 2710.201 kb on - strand, within RR42_RS32600 at 2710.739 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 2,707,052 + RR42_RS32585 0.76 -0.6 2,707,053 - RR42_RS32585 0.76 +0.3 2,707,131 + +0.6 2,707,131 + -1.4 2,707,132 - +0.4 2,707,553 - RR42_RS32590 0.63 -0.9 2,707,773 + -0.9 2,707,774 - -1.5 2,707,861 + -0.4 2,707,861 + -0.6 2,707,943 + -2.2 2,707,991 - -2.6 2,708,122 + RR42_RS32595 0.17 -0.3 2,708,290 + RR42_RS32595 0.25 -0.2 2,708,462 + RR42_RS32595 0.34 -1.1 2,708,462 + RR42_RS32595 0.34 -0.2 2,708,659 + RR42_RS32595 0.44 -2.0 2,708,833 + RR42_RS32595 0.53 -0.7 2,708,909 - RR42_RS32595 0.56 -0.7 2,708,947 + RR42_RS32595 0.58 -0.2 2,708,947 + RR42_RS32595 0.58 -0.3 2,708,948 - RR42_RS32595 0.58 -0.2 2,709,203 - RR42_RS32595 0.71 -0.8 2,709,229 + RR42_RS32595 0.73 -2.5 2,709,475 + RR42_RS32595 0.85 -0.6 2,709,589 + +2.1 2,709,590 - -1.3 2,709,662 - -2.5 2,709,775 - -1.2 2,709,915 + RR42_RS32600 0.14 -0.4 2,709,916 - RR42_RS32600 0.14 -0.2 2,709,937 - RR42_RS32600 0.16 -1.2 2,710,200 + RR42_RS32600 0.43 -1.7 2,710,200 + RR42_RS32600 0.43 +0.8 2,710,201 - RR42_RS32600 0.44 -0.5 2,710,739 + -2.0
Or see this region's nucleotide sequence