Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32580 and RR42_RS32585 are separated by 391 nucleotides RR42_RS32585 and RR42_RS32590 overlap by 1 nucleotides RR42_RS32590 and RR42_RS32595 are separated by 3 nucleotides
RR42_RS32580: RR42_RS32580 - GntR family transcriptional regulator, at 2,705,601 to 2,706,383
_RS32580
RR42_RS32585: RR42_RS32585 - cytochrome C oxidase, at 2,706,775 to 2,707,140
_RS32585
RR42_RS32590: RR42_RS32590 - cytochrome o ubiquinol oxidase subunit III, at 2,707,140 to 2,707,790
_RS32590
RR42_RS32595: RR42_RS32595 - cytochrome o ubiquinol oxidase subunit I, at 2,707,794 to 2,709,770
_RS32595
Position (kb)
2707
2708 Strain fitness (log2 ratio)
-2
-1
0
1 at 2706.254 kb on + strand, within RR42_RS32580 at 2706.254 kb on + strand, within RR42_RS32580 at 2706.254 kb on + strand, within RR42_RS32580 at 2706.397 kb on + strand at 2706.398 kb on - strand at 2706.398 kb on - strand at 2706.760 kb on - strand at 2706.760 kb on - strand at 2706.769 kb on + strand at 2706.789 kb on + strand at 2707.052 kb on + strand, within RR42_RS32585 at 2707.053 kb on - strand, within RR42_RS32585 at 2707.131 kb on + strand at 2707.131 kb on + strand at 2707.132 kb on - strand at 2707.553 kb on - strand, within RR42_RS32590 at 2707.773 kb on + strand at 2707.774 kb on - strand at 2707.861 kb on + strand at 2707.861 kb on + strand at 2707.943 kb on + strand at 2707.991 kb on - strand at 2708.122 kb on + strand, within RR42_RS32595 at 2708.290 kb on + strand, within RR42_RS32595 at 2708.462 kb on + strand, within RR42_RS32595 at 2708.462 kb on + strand, within RR42_RS32595 at 2708.659 kb on + strand, within RR42_RS32595
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 2,706,254 + RR42_RS32580 0.83 +0.5 2,706,254 + RR42_RS32580 0.83 +0.9 2,706,254 + RR42_RS32580 0.83 -0.2 2,706,397 + -1.6 2,706,398 - -0.2 2,706,398 - -2.1 2,706,760 - -0.1 2,706,760 - +0.2 2,706,769 + -0.1 2,706,789 + -1.7 2,707,052 + RR42_RS32585 0.76 -0.6 2,707,053 - RR42_RS32585 0.76 +0.3 2,707,131 + +0.6 2,707,131 + -1.4 2,707,132 - +0.4 2,707,553 - RR42_RS32590 0.63 -0.9 2,707,773 + -0.9 2,707,774 - -1.5 2,707,861 + -0.4 2,707,861 + -0.6 2,707,943 + -2.2 2,707,991 - -2.6 2,708,122 + RR42_RS32595 0.17 -0.3 2,708,290 + RR42_RS32595 0.25 -0.2 2,708,462 + RR42_RS32595 0.34 -1.1 2,708,462 + RR42_RS32595 0.34 -0.2 2,708,659 + RR42_RS32595 0.44 -2.0
Or see this region's nucleotide sequence