Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32455 and RR42_RS32460 are separated by 108 nucleotides RR42_RS32460 and RR42_RS32465 are separated by 210 nucleotides
RR42_RS32455: RR42_RS32455 - oxidoreductase, at 2,678,053 to 2,679,105
_RS32455
RR42_RS32460: RR42_RS32460 - LysR family transcriptional regulator, at 2,679,214 to 2,680,173
_RS32460
RR42_RS32465: RR42_RS32465 - 3-phenylpropionic acid transporter, at 2,680,384 to 2,681,796
_RS32465
Position (kb)
2679
2680
2681 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2678.279 kb on + strand, within RR42_RS32455 at 2678.279 kb on + strand, within RR42_RS32455 at 2678.279 kb on + strand, within RR42_RS32455 at 2678.280 kb on - strand, within RR42_RS32455 at 2678.336 kb on + strand, within RR42_RS32455 at 2678.337 kb on - strand, within RR42_RS32455 at 2678.337 kb on - strand, within RR42_RS32455 at 2678.363 kb on + strand, within RR42_RS32455 at 2678.574 kb on + strand, within RR42_RS32455 at 2678.630 kb on + strand, within RR42_RS32455 at 2678.630 kb on + strand, within RR42_RS32455 at 2678.631 kb on - strand, within RR42_RS32455 at 2678.951 kb on + strand, within RR42_RS32455 at 2678.952 kb on - strand, within RR42_RS32455 at 2678.952 kb on - strand, within RR42_RS32455 at 2679.181 kb on + strand at 2679.181 kb on + strand at 2679.182 kb on - strand at 2679.187 kb on + strand at 2679.188 kb on - strand at 2679.189 kb on + strand at 2679.190 kb on - strand at 2679.266 kb on + strand at 2679.266 kb on + strand at 2679.267 kb on - strand at 2679.304 kb on - strand at 2679.446 kb on + strand, within RR42_RS32460 at 2679.757 kb on + strand, within RR42_RS32460 at 2680.046 kb on + strand, within RR42_RS32460 at 2680.047 kb on - strand at 2680.121 kb on + strand at 2680.122 kb on - strand at 2680.172 kb on - strand at 2680.392 kb on + strand at 2680.475 kb on - strand at 2680.477 kb on + strand at 2680.478 kb on - strand at 2680.492 kb on + strand at 2680.492 kb on + strand at 2680.492 kb on + strand at 2680.492 kb on + strand at 2680.492 kb on + strand at 2680.492 kb on + strand at 2680.492 kb on + strand at 2680.493 kb on - strand at 2680.493 kb on - strand at 2680.579 kb on + strand, within RR42_RS32465 at 2680.579 kb on + strand, within RR42_RS32465 at 2680.580 kb on - strand, within RR42_RS32465 at 2680.580 kb on - strand, within RR42_RS32465 at 2680.580 kb on - strand, within RR42_RS32465 at 2680.580 kb on - strand, within RR42_RS32465 at 2680.591 kb on + strand, within RR42_RS32465 at 2680.592 kb on - strand, within RR42_RS32465 at 2680.592 kb on - strand, within RR42_RS32465 at 2680.658 kb on + strand, within RR42_RS32465 at 2680.659 kb on - strand, within RR42_RS32465 at 2680.659 kb on - strand, within RR42_RS32465 at 2680.659 kb on - strand, within RR42_RS32465 at 2680.659 kb on - strand, within RR42_RS32465 at 2680.720 kb on + strand, within RR42_RS32465 at 2680.721 kb on - strand, within RR42_RS32465 at 2680.721 kb on - strand, within RR42_RS32465 at 2680.721 kb on - strand, within RR42_RS32465 at 2680.758 kb on + strand, within RR42_RS32465 at 2680.852 kb on + strand, within RR42_RS32465 at 2680.927 kb on + strand, within RR42_RS32465 at 2680.930 kb on + strand, within RR42_RS32465 at 2680.930 kb on + strand, within RR42_RS32465 at 2680.930 kb on + strand, within RR42_RS32465 at 2680.931 kb on - strand, within RR42_RS32465 at 2680.931 kb on - strand, within RR42_RS32465 at 2680.931 kb on - strand, within RR42_RS32465 at 2680.931 kb on - strand, within RR42_RS32465 at 2680.931 kb on - strand, within RR42_RS32465
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 2,678,279 + RR42_RS32455 0.21 -2.0 2,678,279 + RR42_RS32455 0.21 +1.2 2,678,279 + RR42_RS32455 0.21 -0.7 2,678,280 - RR42_RS32455 0.22 -0.2 2,678,336 + RR42_RS32455 0.27 -1.8 2,678,337 - RR42_RS32455 0.27 -2.2 2,678,337 - RR42_RS32455 0.27 -0.1 2,678,363 + RR42_RS32455 0.29 +0.2 2,678,574 + RR42_RS32455 0.49 -0.5 2,678,630 + RR42_RS32455 0.55 -0.0 2,678,630 + RR42_RS32455 0.55 -0.3 2,678,631 - RR42_RS32455 0.55 +0.4 2,678,951 + RR42_RS32455 0.85 +0.4 2,678,952 - RR42_RS32455 0.85 +0.5 2,678,952 - RR42_RS32455 0.85 -0.8 2,679,181 + -0.3 2,679,181 + +0.4 2,679,182 - -0.6 2,679,187 + +0.8 2,679,188 - -0.1 2,679,189 + +0.5 2,679,190 - +0.8 2,679,266 + -0.4 2,679,266 + -0.4 2,679,267 - +2.4 2,679,304 - -1.5 2,679,446 + RR42_RS32460 0.24 -0.0 2,679,757 + RR42_RS32460 0.57 +1.3 2,680,046 + RR42_RS32460 0.87 +1.1 2,680,047 - +1.8 2,680,121 + -0.4 2,680,122 - +1.2 2,680,172 - +0.4 2,680,392 + +0.4 2,680,475 - -0.6 2,680,477 + -0.4 2,680,478 - -0.1 2,680,492 + +0.5 2,680,492 + -0.5 2,680,492 + -1.1 2,680,492 + +0.2 2,680,492 + -0.1 2,680,492 + +0.2 2,680,492 + +0.0 2,680,493 - +0.4 2,680,493 - +0.4 2,680,579 + RR42_RS32465 0.14 +0.4 2,680,579 + RR42_RS32465 0.14 +0.6 2,680,580 - RR42_RS32465 0.14 -0.2 2,680,580 - RR42_RS32465 0.14 +0.2 2,680,580 - RR42_RS32465 0.14 -0.8 2,680,580 - RR42_RS32465 0.14 +0.4 2,680,591 + RR42_RS32465 0.15 +0.2 2,680,592 - RR42_RS32465 0.15 +0.2 2,680,592 - RR42_RS32465 0.15 -0.4 2,680,658 + RR42_RS32465 0.19 -0.6 2,680,659 - RR42_RS32465 0.19 -1.6 2,680,659 - RR42_RS32465 0.19 -0.8 2,680,659 - RR42_RS32465 0.19 -0.3 2,680,659 - RR42_RS32465 0.19 -1.6 2,680,720 + RR42_RS32465 0.24 -0.3 2,680,721 - RR42_RS32465 0.24 +0.9 2,680,721 - RR42_RS32465 0.24 +0.8 2,680,721 - RR42_RS32465 0.24 +0.7 2,680,758 + RR42_RS32465 0.26 -0.6 2,680,852 + RR42_RS32465 0.33 -0.6 2,680,927 + RR42_RS32465 0.38 -0.8 2,680,930 + RR42_RS32465 0.39 -0.1 2,680,930 + RR42_RS32465 0.39 +0.0 2,680,930 + RR42_RS32465 0.39 -0.8 2,680,931 - RR42_RS32465 0.39 +1.8 2,680,931 - RR42_RS32465 0.39 +0.1 2,680,931 - RR42_RS32465 0.39 -1.2 2,680,931 - RR42_RS32465 0.39 +1.8 2,680,931 - RR42_RS32465 0.39 +0.0
Or see this region's nucleotide sequence