Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32265 and RR42_RS32270 are separated by 253 nucleotides RR42_RS32270 and RR42_RS32275 are separated by 144 nucleotides
RR42_RS32265: RR42_RS32265 - phytoene synthase, at 2,627,127 to 2,627,975
_RS32265
RR42_RS32270: RR42_RS32270 - hypothetical protein, at 2,628,229 to 2,628,489
_RS32270
RR42_RS32275: RR42_RS32275 - gamma-glutamyltransferase, at 2,628,634 to 2,630,226
_RS32275
Position (kb)
2628
2629 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2627.240 kb on - strand, within RR42_RS32265 at 2627.314 kb on + strand, within RR42_RS32265 at 2627.315 kb on - strand, within RR42_RS32265 at 2627.317 kb on + strand, within RR42_RS32265 at 2627.317 kb on + strand, within RR42_RS32265 at 2627.318 kb on - strand, within RR42_RS32265 at 2627.434 kb on + strand, within RR42_RS32265 at 2627.435 kb on - strand, within RR42_RS32265 at 2627.435 kb on - strand, within RR42_RS32265 at 2627.521 kb on - strand, within RR42_RS32265 at 2627.593 kb on + strand, within RR42_RS32265 at 2627.593 kb on + strand, within RR42_RS32265 at 2627.594 kb on - strand, within RR42_RS32265 at 2627.594 kb on - strand, within RR42_RS32265 at 2627.594 kb on - strand, within RR42_RS32265 at 2627.601 kb on + strand, within RR42_RS32265 at 2627.601 kb on + strand, within RR42_RS32265 at 2627.601 kb on + strand, within RR42_RS32265 at 2627.602 kb on - strand, within RR42_RS32265 at 2627.607 kb on - strand, within RR42_RS32265 at 2627.746 kb on + strand, within RR42_RS32265 at 2627.747 kb on - strand, within RR42_RS32265 at 2627.747 kb on - strand, within RR42_RS32265 at 2627.822 kb on + strand, within RR42_RS32265 at 2627.822 kb on + strand, within RR42_RS32265 at 2627.823 kb on - strand, within RR42_RS32265 at 2627.823 kb on - strand, within RR42_RS32265 at 2627.848 kb on + strand, within RR42_RS32265 at 2627.848 kb on + strand, within RR42_RS32265 at 2627.848 kb on + strand, within RR42_RS32265 at 2627.848 kb on + strand, within RR42_RS32265 at 2627.849 kb on - strand, within RR42_RS32265 at 2627.849 kb on - strand, within RR42_RS32265 at 2627.849 kb on - strand, within RR42_RS32265 at 2627.900 kb on + strand at 2627.977 kb on + strand at 2627.977 kb on + strand at 2627.978 kb on - strand at 2627.978 kb on - strand at 2628.021 kb on - strand at 2628.021 kb on - strand at 2628.021 kb on - strand at 2628.086 kb on + strand at 2628.086 kb on + strand at 2628.088 kb on + strand at 2628.088 kb on + strand at 2628.088 kb on + strand at 2628.088 kb on + strand at 2628.088 kb on + strand at 2628.088 kb on + strand at 2628.088 kb on + strand at 2628.089 kb on - strand at 2628.089 kb on - strand at 2628.089 kb on - strand at 2628.089 kb on - strand at 2628.230 kb on + strand at 2628.231 kb on - strand at 2628.234 kb on + strand at 2628.290 kb on + strand, within RR42_RS32270 at 2628.308 kb on + strand, within RR42_RS32270 at 2628.478 kb on + strand at 2628.478 kb on + strand at 2628.478 kb on + strand at 2628.479 kb on - strand at 2628.479 kb on - strand at 2628.521 kb on + strand at 2628.532 kb on + strand at 2628.532 kb on + strand at 2628.532 kb on + strand at 2628.532 kb on + strand at 2628.532 kb on + strand at 2628.542 kb on + strand at 2629.022 kb on - strand, within RR42_RS32275 at 2629.136 kb on + strand, within RR42_RS32275 at 2629.136 kb on + strand, within RR42_RS32275 at 2629.136 kb on + strand, within RR42_RS32275 at 2629.136 kb on + strand, within RR42_RS32275 at 2629.136 kb on + strand, within RR42_RS32275 at 2629.137 kb on - strand, within RR42_RS32275 at 2629.137 kb on - strand, within RR42_RS32275 at 2629.175 kb on + strand, within RR42_RS32275 at 2629.175 kb on + strand, within RR42_RS32275 at 2629.176 kb on - strand, within RR42_RS32275 at 2629.176 kb on - strand, within RR42_RS32275 at 2629.176 kb on - strand, within RR42_RS32275 at 2629.259 kb on + strand, within RR42_RS32275 at 2629.260 kb on - strand, within RR42_RS32275 at 2629.319 kb on + strand, within RR42_RS32275 at 2629.319 kb on + strand, within RR42_RS32275 at 2629.319 kb on + strand, within RR42_RS32275
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 2,627,240 - RR42_RS32265 0.13 +0.7 2,627,314 + RR42_RS32265 0.22 +0.3 2,627,315 - RR42_RS32265 0.22 -0.2 2,627,317 + RR42_RS32265 0.22 +0.1 2,627,317 + RR42_RS32265 0.22 +0.9 2,627,318 - RR42_RS32265 0.22 -0.3 2,627,434 + RR42_RS32265 0.36 +0.8 2,627,435 - RR42_RS32265 0.36 -0.6 2,627,435 - RR42_RS32265 0.36 -0.3 2,627,521 - RR42_RS32265 0.46 -2.9 2,627,593 + RR42_RS32265 0.55 +1.8 2,627,593 + RR42_RS32265 0.55 -0.6 2,627,594 - RR42_RS32265 0.55 +0.2 2,627,594 - RR42_RS32265 0.55 -0.6 2,627,594 - RR42_RS32265 0.55 -1.2 2,627,601 + RR42_RS32265 0.56 -0.1 2,627,601 + RR42_RS32265 0.56 -0.0 2,627,601 + RR42_RS32265 0.56 -1.2 2,627,602 - RR42_RS32265 0.56 -0.4 2,627,607 - RR42_RS32265 0.57 -0.1 2,627,746 + RR42_RS32265 0.73 -1.0 2,627,747 - RR42_RS32265 0.73 +1.1 2,627,747 - RR42_RS32265 0.73 -1.9 2,627,822 + RR42_RS32265 0.82 +1.1 2,627,822 + RR42_RS32265 0.82 -1.6 2,627,823 - RR42_RS32265 0.82 +0.0 2,627,823 - RR42_RS32265 0.82 -1.7 2,627,848 + RR42_RS32265 0.85 -1.0 2,627,848 + RR42_RS32265 0.85 -0.2 2,627,848 + RR42_RS32265 0.85 -0.2 2,627,848 + RR42_RS32265 0.85 -0.1 2,627,849 - RR42_RS32265 0.85 -0.5 2,627,849 - RR42_RS32265 0.85 +0.7 2,627,849 - RR42_RS32265 0.85 -0.5 2,627,900 + +0.1 2,627,977 + -0.6 2,627,977 + -1.7 2,627,978 - -0.7 2,627,978 - +0.0 2,628,021 - -0.0 2,628,021 - -0.8 2,628,021 - -0.1 2,628,086 + +0.5 2,628,086 + -0.5 2,628,088 + -0.9 2,628,088 + +0.7 2,628,088 + +0.1 2,628,088 + +1.5 2,628,088 + -0.0 2,628,088 + -0.7 2,628,088 + -0.1 2,628,089 - +0.2 2,628,089 - +0.4 2,628,089 - -0.3 2,628,089 - -0.0 2,628,230 + -1.2 2,628,231 - -0.7 2,628,234 + +0.3 2,628,290 + RR42_RS32270 0.23 -1.5 2,628,308 + RR42_RS32270 0.30 -0.6 2,628,478 + -0.2 2,628,478 + -0.4 2,628,478 + -0.2 2,628,479 - -0.1 2,628,479 - +0.0 2,628,521 + +0.5 2,628,532 + +0.4 2,628,532 + +1.1 2,628,532 + -2.7 2,628,532 + -0.9 2,628,532 + -0.5 2,628,542 + -1.2 2,629,022 - RR42_RS32275 0.24 +0.7 2,629,136 + RR42_RS32275 0.32 -0.2 2,629,136 + RR42_RS32275 0.32 +0.8 2,629,136 + RR42_RS32275 0.32 -0.2 2,629,136 + RR42_RS32275 0.32 -1.3 2,629,136 + RR42_RS32275 0.32 +0.3 2,629,137 - RR42_RS32275 0.32 +0.3 2,629,137 - RR42_RS32275 0.32 -2.1 2,629,175 + RR42_RS32275 0.34 -0.9 2,629,175 + RR42_RS32275 0.34 -0.0 2,629,176 - RR42_RS32275 0.34 -0.3 2,629,176 - RR42_RS32275 0.34 +0.6 2,629,176 - RR42_RS32275 0.34 -0.1 2,629,259 + RR42_RS32275 0.39 +0.7 2,629,260 - RR42_RS32275 0.39 -1.2 2,629,319 + RR42_RS32275 0.43 +0.3 2,629,319 + RR42_RS32275 0.43 +0.0 2,629,319 + RR42_RS32275 0.43 +0.7
Or see this region's nucleotide sequence