Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31950 and RR42_RS31955 are separated by 26 nucleotides RR42_RS31955 and RR42_RS31960 are separated by 4 nucleotides RR42_RS31960 and RR42_RS31965 are separated by 417 nucleotides
RR42_RS31950: RR42_RS31950 - ketoacid CoA transferase, at 2,544,821 to 2,545,624
_RS31950
RR42_RS31955: RR42_RS31955 - CoA-transferase, at 2,545,651 to 2,546,541
_RS31955
RR42_RS31960: RR42_RS31960 - biphenyl 2,3-dioxygenase, at 2,546,546 to 2,547,442
_RS31960
RR42_RS31965: RR42_RS31965 - LuxR family transcriptional regulator, at 2,547,860 to 2,549,059
_RS31965
Position (kb)
2546
2547
2548 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2545.710 kb on - strand at 2545.710 kb on - strand at 2545.746 kb on - strand, within RR42_RS31955 at 2545.784 kb on + strand, within RR42_RS31955 at 2545.814 kb on + strand, within RR42_RS31955 at 2545.955 kb on + strand, within RR42_RS31955 at 2545.955 kb on + strand, within RR42_RS31955 at 2545.955 kb on + strand, within RR42_RS31955 at 2545.956 kb on - strand, within RR42_RS31955 at 2545.956 kb on - strand, within RR42_RS31955 at 2545.956 kb on - strand, within RR42_RS31955 at 2546.467 kb on - strand at 2546.541 kb on + strand at 2546.541 kb on + strand at 2546.680 kb on - strand, within RR42_RS31960 at 2546.971 kb on - strand, within RR42_RS31960 at 2547.321 kb on + strand, within RR42_RS31960 at 2547.375 kb on + strand at 2547.416 kb on - strand at 2547.859 kb on + strand at 2547.859 kb on + strand at 2547.860 kb on - strand at 2547.860 kb on - strand at 2547.860 kb on - strand at 2547.919 kb on + strand at 2547.920 kb on - strand at 2547.920 kb on - strand at 2548.103 kb on + strand, within RR42_RS31965 at 2548.103 kb on + strand, within RR42_RS31965 at 2548.104 kb on - strand, within RR42_RS31965 at 2548.104 kb on - strand, within RR42_RS31965 at 2548.124 kb on + strand, within RR42_RS31965 at 2548.258 kb on + strand, within RR42_RS31965 at 2548.265 kb on + strand, within RR42_RS31965 at 2548.266 kb on - strand, within RR42_RS31965
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 2,545,710 - -0.8 2,545,710 - -0.0 2,545,746 - RR42_RS31955 0.11 +1.1 2,545,784 + RR42_RS31955 0.15 -0.1 2,545,814 + RR42_RS31955 0.18 +0.6 2,545,955 + RR42_RS31955 0.34 +0.2 2,545,955 + RR42_RS31955 0.34 +0.8 2,545,955 + RR42_RS31955 0.34 +0.3 2,545,956 - RR42_RS31955 0.34 -0.0 2,545,956 - RR42_RS31955 0.34 +0.4 2,545,956 - RR42_RS31955 0.34 -0.1 2,546,467 - -0.0 2,546,541 + +0.0 2,546,541 + +0.9 2,546,680 - RR42_RS31960 0.15 -0.3 2,546,971 - RR42_RS31960 0.47 -0.8 2,547,321 + RR42_RS31960 0.86 -1.2 2,547,375 + +0.0 2,547,416 - +0.5 2,547,859 + -0.2 2,547,859 + +0.5 2,547,860 - -0.0 2,547,860 - -0.6 2,547,860 - +1.8 2,547,919 + -1.2 2,547,920 - +0.1 2,547,920 - +0.0 2,548,103 + RR42_RS31965 0.20 +0.1 2,548,103 + RR42_RS31965 0.20 -2.7 2,548,104 - RR42_RS31965 0.20 -0.6 2,548,104 - RR42_RS31965 0.20 -0.1 2,548,124 + RR42_RS31965 0.22 +1.8 2,548,258 + RR42_RS31965 0.33 +1.5 2,548,265 + RR42_RS31965 0.34 -0.7 2,548,266 - RR42_RS31965 0.34 -0.2
Or see this region's nucleotide sequence