Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31930 and RR42_RS31935 are separated by 9 nucleotides RR42_RS31935 and RR42_RS31940 are separated by 45 nucleotides RR42_RS31940 and RR42_RS31945 overlap by 4 nucleotides RR42_RS31945 and RR42_RS31950 are separated by 8 nucleotides
RR42_RS31930: RR42_RS31930 - acyl-CoA dehydrogenase, at 2,540,806 to 2,541,975
_RS31930
RR42_RS31935: RR42_RS31935 - enoyl-CoA hydratase, at 2,541,985 to 2,542,770
_RS31935
RR42_RS31940: RR42_RS31940 - 2-nitropropane dioxygenase, at 2,542,816 to 2,543,925
_RS31940
RR42_RS31945: RR42_RS31945 - enoyl-CoA hydratase, at 2,543,922 to 2,544,812
_RS31945
RR42_RS31950: RR42_RS31950 - ketoacid CoA transferase, at 2,544,821 to 2,545,624
_RS31950
Position (kb)
2542
2543
2544 Strain fitness (log2 ratio)
-1
0
1 at 2541.962 kb on + strand at 2542.074 kb on + strand, within RR42_RS31935 at 2542.075 kb on - strand, within RR42_RS31935 at 2542.077 kb on - strand, within RR42_RS31935 at 2542.110 kb on - strand, within RR42_RS31935 at 2542.302 kb on - strand, within RR42_RS31935 at 2542.302 kb on - strand, within RR42_RS31935 at 2542.416 kb on + strand, within RR42_RS31935 at 2542.517 kb on + strand, within RR42_RS31935 at 2542.518 kb on - strand, within RR42_RS31935 at 2542.775 kb on - strand at 2543.060 kb on + strand, within RR42_RS31940 at 2543.282 kb on + strand, within RR42_RS31940 at 2543.698 kb on - strand, within RR42_RS31940 at 2543.904 kb on + strand at 2543.904 kb on + strand at 2544.281 kb on - strand, within RR42_RS31945 at 2544.470 kb on - strand, within RR42_RS31945 at 2544.470 kb on - strand, within RR42_RS31945 at 2544.491 kb on - strand, within RR42_RS31945
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 2,541,962 + +0.0 2,542,074 + RR42_RS31935 0.11 +0.2 2,542,075 - RR42_RS31935 0.11 +0.8 2,542,077 - RR42_RS31935 0.12 -1.1 2,542,110 - RR42_RS31935 0.16 -0.2 2,542,302 - RR42_RS31935 0.40 +0.8 2,542,302 - RR42_RS31935 0.40 +1.6 2,542,416 + RR42_RS31935 0.55 -1.1 2,542,517 + RR42_RS31935 0.68 +0.5 2,542,518 - RR42_RS31935 0.68 -0.6 2,542,775 - +1.3 2,543,060 + RR42_RS31940 0.22 +0.1 2,543,282 + RR42_RS31940 0.42 +1.1 2,543,698 - RR42_RS31940 0.79 +1.2 2,543,904 + +0.8 2,543,904 + +0.4 2,544,281 - RR42_RS31945 0.40 +0.5 2,544,470 - RR42_RS31945 0.62 +0.6 2,544,470 - RR42_RS31945 0.62 -1.0 2,544,491 - RR42_RS31945 0.64 -0.3
Or see this region's nucleotide sequence