Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31485 and RR42_RS31490 are separated by 401 nucleotides RR42_RS31490 and RR42_RS31495 are separated by 32 nucleotides RR42_RS31495 and RR42_RS31500 are separated by 224 nucleotides RR42_RS31500 and RR42_RS31505 are separated by 112 nucleotides
RR42_RS31485: RR42_RS31485 - membrane protein, at 2,444,951 to 2,445,295
_RS31485
RR42_RS31490: RR42_RS31490 - hypothetical protein, at 2,445,697 to 2,446,218
_RS31490
RR42_RS31495: RR42_RS31495 - Cro/Cl family transcriptional regulator, at 2,446,251 to 2,446,478
_RS31495
RR42_RS31500: RR42_RS31500 - hypothetical protein, at 2,446,703 to 2,446,963
_RS31500
RR42_RS31505: RR42_RS31505 - hypothetical protein, at 2,447,076 to 2,447,405
_RS31505
Position (kb)
2446
2447 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2445.328 kb on + strand at 2445.329 kb on - strand at 2445.329 kb on - strand at 2445.329 kb on - strand at 2445.352 kb on - strand at 2445.393 kb on + strand at 2445.393 kb on + strand at 2445.394 kb on - strand at 2445.394 kb on - strand at 2445.414 kb on + strand at 2445.415 kb on - strand at 2445.434 kb on + strand at 2445.434 kb on + strand at 2445.435 kb on - strand at 2445.435 kb on - strand at 2445.435 kb on - strand at 2445.440 kb on + strand at 2445.441 kb on - strand at 2445.441 kb on - strand at 2445.696 kb on - strand at 2446.105 kb on + strand, within RR42_RS31490 at 2446.106 kb on - strand, within RR42_RS31490 at 2446.106 kb on - strand, within RR42_RS31490 at 2446.250 kb on + strand at 2446.250 kb on + strand at 2446.250 kb on + strand at 2446.250 kb on + strand at 2446.251 kb on - strand at 2446.251 kb on - strand at 2446.251 kb on - strand at 2446.257 kb on - strand at 2446.388 kb on + strand, within RR42_RS31495 at 2446.388 kb on + strand, within RR42_RS31495 at 2446.455 kb on - strand, within RR42_RS31495 at 2446.649 kb on + strand at 2446.649 kb on + strand at 2446.668 kb on + strand at 2446.668 kb on + strand at 2446.668 kb on + strand at 2446.669 kb on - strand at 2446.702 kb on + strand at 2446.703 kb on - strand at 2446.703 kb on - strand at 2446.703 kb on - strand at 2446.703 kb on - strand at 2446.703 kb on - strand at 2446.718 kb on + strand at 2446.718 kb on + strand at 2446.719 kb on - strand at 2446.719 kb on - strand at 2446.719 kb on - strand at 2446.983 kb on + strand at 2447.026 kb on + strand at 2447.027 kb on - strand at 2447.106 kb on + strand at 2447.106 kb on + strand at 2447.107 kb on - strand at 2447.107 kb on - strand at 2447.107 kb on - strand at 2447.144 kb on - strand, within RR42_RS31505 at 2447.199 kb on + strand, within RR42_RS31505 at 2447.389 kb on - strand at 2447.403 kb on + strand at 2447.403 kb on + strand at 2447.403 kb on + strand at 2447.404 kb on - strand at 2447.404 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 2,445,328 + -0.1 2,445,329 - -1.3 2,445,329 - -0.3 2,445,329 - +3.5 2,445,352 - +0.4 2,445,393 + +0.0 2,445,393 + -0.0 2,445,394 - -0.3 2,445,394 - -0.5 2,445,414 + -0.1 2,445,415 - +0.4 2,445,434 + -0.8 2,445,434 + +0.1 2,445,435 - +0.1 2,445,435 - +0.4 2,445,435 - +0.5 2,445,440 + -0.0 2,445,441 - -0.3 2,445,441 - -0.1 2,445,696 - +0.7 2,446,105 + RR42_RS31490 0.78 +0.3 2,446,106 - RR42_RS31490 0.78 -0.3 2,446,106 - RR42_RS31490 0.78 +0.0 2,446,250 + -0.8 2,446,250 + -0.4 2,446,250 + +0.4 2,446,250 + +1.0 2,446,251 - +0.3 2,446,251 - +0.7 2,446,251 - +0.5 2,446,257 - -0.3 2,446,388 + RR42_RS31495 0.60 -0.6 2,446,388 + RR42_RS31495 0.60 +1.8 2,446,455 - RR42_RS31495 0.89 +0.1 2,446,649 + +0.4 2,446,649 + +0.4 2,446,668 + +2.4 2,446,668 + +0.8 2,446,668 + +1.9 2,446,669 - -1.2 2,446,702 + +0.1 2,446,703 - -0.1 2,446,703 - +0.4 2,446,703 - +0.1 2,446,703 - +0.3 2,446,703 - +1.0 2,446,718 + +0.9 2,446,718 + +0.0 2,446,719 - +1.0 2,446,719 - -1.4 2,446,719 - +0.9 2,446,983 + +0.2 2,447,026 + -0.1 2,447,027 - +1.0 2,447,106 + -0.2 2,447,106 + -1.6 2,447,107 - -0.4 2,447,107 - +0.3 2,447,107 - -0.0 2,447,144 - RR42_RS31505 0.21 +0.8 2,447,199 + RR42_RS31505 0.37 +0.9 2,447,389 - +0.2 2,447,403 + -0.7 2,447,403 + -0.8 2,447,403 + +1.6 2,447,404 - -0.9 2,447,404 - +0.6
Or see this region's nucleotide sequence