Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31010 and RR42_RS31015 are separated by 27 nucleotides RR42_RS31015 and RR42_RS31020 are separated by 6 nucleotides RR42_RS31020 and RR42_RS31025 are separated by 10 nucleotides
RR42_RS31010: RR42_RS31010 - phosphatidate cytidylyltransferase, at 2,338,320 to 2,339,261
_RS31010
RR42_RS31015: RR42_RS31015 - acyl-phosphate glycerol 3-phosphate acyltransferase, at 2,339,289 to 2,339,915
_RS31015
RR42_RS31020: RR42_RS31020 - hypothetical protein, at 2,339,922 to 2,340,362
_RS31020
RR42_RS31025: RR42_RS31025 - serine/threonine protein phosphatase, at 2,340,373 to 2,341,806
_RS31025
Position (kb)
2339
2340
2341 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2338.927 kb on + strand, within RR42_RS31010 at 2338.939 kb on + strand, within RR42_RS31010 at 2338.939 kb on + strand, within RR42_RS31010 at 2338.940 kb on - strand, within RR42_RS31010 at 2338.940 kb on - strand, within RR42_RS31010 at 2338.940 kb on - strand, within RR42_RS31010 at 2339.047 kb on + strand, within RR42_RS31010 at 2339.048 kb on - strand, within RR42_RS31010 at 2339.048 kb on - strand, within RR42_RS31010 at 2339.218 kb on - strand at 2339.290 kb on + strand at 2339.503 kb on + strand, within RR42_RS31015 at 2339.503 kb on + strand, within RR42_RS31015 at 2339.504 kb on - strand, within RR42_RS31015 at 2339.504 kb on - strand, within RR42_RS31015 at 2339.504 kb on - strand, within RR42_RS31015 at 2339.543 kb on + strand, within RR42_RS31015 at 2339.543 kb on + strand, within RR42_RS31015 at 2339.543 kb on + strand, within RR42_RS31015 at 2339.544 kb on - strand, within RR42_RS31015 at 2339.664 kb on + strand, within RR42_RS31015 at 2339.664 kb on + strand, within RR42_RS31015 at 2339.664 kb on + strand, within RR42_RS31015 at 2339.664 kb on + strand, within RR42_RS31015 at 2339.664 kb on + strand, within RR42_RS31015 at 2339.665 kb on - strand, within RR42_RS31015 at 2339.806 kb on + strand, within RR42_RS31015 at 2339.806 kb on + strand, within RR42_RS31015 at 2339.810 kb on + strand, within RR42_RS31015 at 2339.810 kb on + strand, within RR42_RS31015 at 2339.811 kb on - strand, within RR42_RS31015 at 2339.811 kb on - strand, within RR42_RS31015 at 2339.924 kb on - strand at 2339.938 kb on - strand at 2339.938 kb on - strand at 2339.938 kb on - strand at 2339.938 kb on - strand at 2339.938 kb on - strand at 2340.220 kb on + strand, within RR42_RS31020 at 2340.220 kb on + strand, within RR42_RS31020 at 2340.220 kb on + strand, within RR42_RS31020 at 2340.221 kb on - strand, within RR42_RS31020 at 2340.221 kb on - strand, within RR42_RS31020 at 2340.581 kb on + strand, within RR42_RS31025 at 2340.598 kb on + strand, within RR42_RS31025 at 2340.598 kb on + strand, within RR42_RS31025 at 2340.598 kb on + strand, within RR42_RS31025 at 2340.598 kb on + strand, within RR42_RS31025 at 2340.598 kb on + strand, within RR42_RS31025 at 2340.598 kb on + strand, within RR42_RS31025 at 2340.598 kb on + strand, within RR42_RS31025 at 2340.598 kb on + strand, within RR42_RS31025 at 2340.598 kb on + strand, within RR42_RS31025 at 2340.599 kb on - strand, within RR42_RS31025 at 2340.599 kb on - strand, within RR42_RS31025 at 2340.599 kb on - strand, within RR42_RS31025 at 2340.599 kb on - strand, within RR42_RS31025 at 2340.599 kb on - strand, within RR42_RS31025 at 2340.599 kb on - strand, within RR42_RS31025 at 2340.704 kb on + strand, within RR42_RS31025 at 2340.884 kb on + strand, within RR42_RS31025 at 2340.965 kb on + strand, within RR42_RS31025 at 2340.965 kb on + strand, within RR42_RS31025 at 2341.218 kb on - strand, within RR42_RS31025 at 2341.218 kb on - strand, within RR42_RS31025 at 2341.299 kb on - strand, within RR42_RS31025 at 2341.299 kb on - strand, within RR42_RS31025
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 2,338,927 + RR42_RS31010 0.64 +0.4 2,338,939 + RR42_RS31010 0.66 -0.4 2,338,939 + RR42_RS31010 0.66 +0.8 2,338,940 - RR42_RS31010 0.66 -0.5 2,338,940 - RR42_RS31010 0.66 -1.1 2,338,940 - RR42_RS31010 0.66 -0.0 2,339,047 + RR42_RS31010 0.77 -1.1 2,339,048 - RR42_RS31010 0.77 +0.3 2,339,048 - RR42_RS31010 0.77 -1.0 2,339,218 - -1.2 2,339,290 + -0.6 2,339,503 + RR42_RS31015 0.34 -0.2 2,339,503 + RR42_RS31015 0.34 -0.1 2,339,504 - RR42_RS31015 0.34 +0.3 2,339,504 - RR42_RS31015 0.34 +0.3 2,339,504 - RR42_RS31015 0.34 -0.7 2,339,543 + RR42_RS31015 0.41 -1.1 2,339,543 + RR42_RS31015 0.41 +1.6 2,339,543 + RR42_RS31015 0.41 +1.9 2,339,544 - RR42_RS31015 0.41 -1.4 2,339,664 + RR42_RS31015 0.60 -0.5 2,339,664 + RR42_RS31015 0.60 +1.5 2,339,664 + RR42_RS31015 0.60 -0.4 2,339,664 + RR42_RS31015 0.60 -0.5 2,339,664 + RR42_RS31015 0.60 -0.4 2,339,665 - RR42_RS31015 0.60 -2.7 2,339,806 + RR42_RS31015 0.82 -0.4 2,339,806 + RR42_RS31015 0.82 +0.9 2,339,810 + RR42_RS31015 0.83 +1.8 2,339,810 + RR42_RS31015 0.83 -0.3 2,339,811 - RR42_RS31015 0.83 +0.9 2,339,811 - RR42_RS31015 0.83 -0.0 2,339,924 - -0.9 2,339,938 - -0.8 2,339,938 - +0.1 2,339,938 - -1.7 2,339,938 - +0.1 2,339,938 - +0.2 2,340,220 + RR42_RS31020 0.68 +1.4 2,340,220 + RR42_RS31020 0.68 +0.2 2,340,220 + RR42_RS31020 0.68 +0.6 2,340,221 - RR42_RS31020 0.68 +0.2 2,340,221 - RR42_RS31020 0.68 +0.8 2,340,581 + RR42_RS31025 0.15 -0.4 2,340,598 + RR42_RS31025 0.16 +0.4 2,340,598 + RR42_RS31025 0.16 -1.1 2,340,598 + RR42_RS31025 0.16 +0.3 2,340,598 + RR42_RS31025 0.16 -0.7 2,340,598 + RR42_RS31025 0.16 -1.2 2,340,598 + RR42_RS31025 0.16 -0.1 2,340,598 + RR42_RS31025 0.16 -1.4 2,340,598 + RR42_RS31025 0.16 -1.7 2,340,598 + RR42_RS31025 0.16 +0.6 2,340,599 - RR42_RS31025 0.16 -0.0 2,340,599 - RR42_RS31025 0.16 +0.2 2,340,599 - RR42_RS31025 0.16 +0.4 2,340,599 - RR42_RS31025 0.16 -0.5 2,340,599 - RR42_RS31025 0.16 +0.6 2,340,599 - RR42_RS31025 0.16 +0.4 2,340,704 + RR42_RS31025 0.23 +0.8 2,340,884 + RR42_RS31025 0.36 -1.2 2,340,965 + RR42_RS31025 0.41 -0.4 2,340,965 + RR42_RS31025 0.41 +0.5 2,341,218 - RR42_RS31025 0.59 +0.1 2,341,218 - RR42_RS31025 0.59 +0.2 2,341,299 - RR42_RS31025 0.65 -0.0 2,341,299 - RR42_RS31025 0.65 +0.2
Or see this region's nucleotide sequence