Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS30985 and RR42_RS30990 are separated by 250 nucleotides RR42_RS30990 and RR42_RS30995 are separated by 60 nucleotides RR42_RS30995 and RR42_RS31000 are separated by 26 nucleotides
RR42_RS30985: RR42_RS30985 - histone-like nucleoid-structuring protein, at 2,333,850 to 2,334,182
_RS30985
RR42_RS30990: RR42_RS30990 - membrane protein, at 2,334,433 to 2,334,831
_RS30990
RR42_RS30995: RR42_RS30995 - hypothetical protein, at 2,334,892 to 2,335,635
_RS30995
RR42_RS31000: RR42_RS31000 - glutathionylspermidine synthase, at 2,335,662 to 2,336,834
_RS31000
Position (kb)
2334
2335
2336 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2334.156 kb on + strand at 2334.156 kb on + strand at 2334.192 kb on - strand at 2334.243 kb on - strand at 2334.279 kb on + strand at 2334.434 kb on + strand at 2334.476 kb on - strand, within RR42_RS30990 at 2334.560 kb on + strand, within RR42_RS30990 at 2334.560 kb on + strand, within RR42_RS30990 at 2334.560 kb on + strand, within RR42_RS30990 at 2334.709 kb on + strand, within RR42_RS30990 at 2334.709 kb on + strand, within RR42_RS30990 at 2334.710 kb on - strand, within RR42_RS30990 at 2334.746 kb on + strand, within RR42_RS30990 at 2334.746 kb on + strand, within RR42_RS30990 at 2334.746 kb on + strand, within RR42_RS30990 at 2334.747 kb on - strand, within RR42_RS30990 at 2334.748 kb on + strand, within RR42_RS30990 at 2334.749 kb on - strand, within RR42_RS30990 at 2334.791 kb on + strand, within RR42_RS30990 at 2334.791 kb on + strand, within RR42_RS30990 at 2334.792 kb on - strand at 2334.792 kb on - strand at 2334.800 kb on + strand at 2334.801 kb on - strand at 2334.806 kb on + strand at 2334.807 kb on - strand at 2334.807 kb on - strand at 2334.807 kb on - strand at 2334.860 kb on + strand at 2334.860 kb on + strand at 2335.109 kb on + strand, within RR42_RS30995 at 2335.109 kb on + strand, within RR42_RS30995 at 2335.109 kb on + strand, within RR42_RS30995 at 2335.110 kb on - strand, within RR42_RS30995 at 2335.110 kb on - strand, within RR42_RS30995 at 2335.196 kb on + strand, within RR42_RS30995 at 2335.197 kb on - strand, within RR42_RS30995 at 2335.199 kb on + strand, within RR42_RS30995 at 2335.232 kb on + strand, within RR42_RS30995 at 2335.241 kb on + strand, within RR42_RS30995 at 2335.242 kb on - strand, within RR42_RS30995 at 2335.242 kb on - strand, within RR42_RS30995 at 2335.242 kb on - strand, within RR42_RS30995 at 2335.322 kb on + strand, within RR42_RS30995 at 2335.322 kb on + strand, within RR42_RS30995 at 2335.323 kb on - strand, within RR42_RS30995 at 2335.323 kb on - strand, within RR42_RS30995 at 2335.398 kb on - strand, within RR42_RS30995 at 2335.398 kb on - strand, within RR42_RS30995 at 2335.678 kb on + strand at 2335.767 kb on + strand at 2335.768 kb on - strand at 2335.783 kb on + strand, within RR42_RS31000 at 2335.784 kb on - strand, within RR42_RS31000 at 2335.813 kb on + strand, within RR42_RS31000 at 2335.813 kb on + strand, within RR42_RS31000 at 2335.814 kb on - strand, within RR42_RS31000 at 2335.823 kb on - strand, within RR42_RS31000 at 2335.823 kb on - strand, within RR42_RS31000 at 2335.826 kb on + strand, within RR42_RS31000 at 2335.826 kb on + strand, within RR42_RS31000 at 2335.826 kb on + strand, within RR42_RS31000 at 2335.827 kb on - strand, within RR42_RS31000 at 2335.827 kb on - strand, within RR42_RS31000 at 2335.827 kb on - strand, within RR42_RS31000 at 2335.843 kb on + strand, within RR42_RS31000 at 2335.927 kb on + strand, within RR42_RS31000 at 2335.928 kb on - strand, within RR42_RS31000 at 2336.089 kb on + strand, within RR42_RS31000 at 2336.126 kb on - strand, within RR42_RS31000 at 2336.162 kb on - strand, within RR42_RS31000 at 2336.162 kb on - strand, within RR42_RS31000 at 2336.162 kb on - strand, within RR42_RS31000 at 2336.367 kb on - strand, within RR42_RS31000 at 2336.448 kb on - strand, within RR42_RS31000 at 2336.464 kb on + strand, within RR42_RS31000 at 2336.464 kb on + strand, within RR42_RS31000 at 2336.465 kb on - strand, within RR42_RS31000
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 2,334,156 + -0.6 2,334,156 + -0.9 2,334,192 - -0.7 2,334,243 - +2.3 2,334,279 + -0.6 2,334,434 + +0.3 2,334,476 - RR42_RS30990 0.11 -0.0 2,334,560 + RR42_RS30990 0.32 -0.8 2,334,560 + RR42_RS30990 0.32 -1.2 2,334,560 + RR42_RS30990 0.32 +0.6 2,334,709 + RR42_RS30990 0.69 -0.4 2,334,709 + RR42_RS30990 0.69 +0.5 2,334,710 - RR42_RS30990 0.69 +0.6 2,334,746 + RR42_RS30990 0.78 -2.9 2,334,746 + RR42_RS30990 0.78 -2.4 2,334,746 + RR42_RS30990 0.78 -0.0 2,334,747 - RR42_RS30990 0.79 -0.9 2,334,748 + RR42_RS30990 0.79 +0.4 2,334,749 - RR42_RS30990 0.79 -0.5 2,334,791 + RR42_RS30990 0.90 +0.7 2,334,791 + RR42_RS30990 0.90 -1.3 2,334,792 - -1.2 2,334,792 - +0.4 2,334,800 + -0.1 2,334,801 - +0.6 2,334,806 + +0.7 2,334,807 - -0.2 2,334,807 - -0.4 2,334,807 - +0.1 2,334,860 + -0.1 2,334,860 + +2.0 2,335,109 + RR42_RS30995 0.29 -1.0 2,335,109 + RR42_RS30995 0.29 -0.1 2,335,109 + RR42_RS30995 0.29 +0.5 2,335,110 - RR42_RS30995 0.29 +0.7 2,335,110 - RR42_RS30995 0.29 +0.3 2,335,196 + RR42_RS30995 0.41 +0.3 2,335,197 - RR42_RS30995 0.41 +0.6 2,335,199 + RR42_RS30995 0.41 -0.3 2,335,232 + RR42_RS30995 0.46 +0.6 2,335,241 + RR42_RS30995 0.47 -0.0 2,335,242 - RR42_RS30995 0.47 +1.0 2,335,242 - RR42_RS30995 0.47 +1.8 2,335,242 - RR42_RS30995 0.47 -0.4 2,335,322 + RR42_RS30995 0.58 +0.3 2,335,322 + RR42_RS30995 0.58 +0.3 2,335,323 - RR42_RS30995 0.58 -0.7 2,335,323 - RR42_RS30995 0.58 +0.2 2,335,398 - RR42_RS30995 0.68 -0.2 2,335,398 - RR42_RS30995 0.68 +0.5 2,335,678 + -0.4 2,335,767 + +0.4 2,335,768 - -0.1 2,335,783 + RR42_RS31000 0.10 +0.2 2,335,784 - RR42_RS31000 0.10 +0.3 2,335,813 + RR42_RS31000 0.13 -0.7 2,335,813 + RR42_RS31000 0.13 +0.4 2,335,814 - RR42_RS31000 0.13 +0.9 2,335,823 - RR42_RS31000 0.14 -0.1 2,335,823 - RR42_RS31000 0.14 -0.9 2,335,826 + RR42_RS31000 0.14 -0.2 2,335,826 + RR42_RS31000 0.14 -0.4 2,335,826 + RR42_RS31000 0.14 +0.4 2,335,827 - RR42_RS31000 0.14 +3.0 2,335,827 - RR42_RS31000 0.14 -0.3 2,335,827 - RR42_RS31000 0.14 -0.1 2,335,843 + RR42_RS31000 0.15 +0.6 2,335,927 + RR42_RS31000 0.23 -0.0 2,335,928 - RR42_RS31000 0.23 -0.2 2,336,089 + RR42_RS31000 0.36 +0.1 2,336,126 - RR42_RS31000 0.40 -1.4 2,336,162 - RR42_RS31000 0.43 +0.2 2,336,162 - RR42_RS31000 0.43 +0.2 2,336,162 - RR42_RS31000 0.43 -0.9 2,336,367 - RR42_RS31000 0.60 +1.3 2,336,448 - RR42_RS31000 0.67 +0.1 2,336,464 + RR42_RS31000 0.68 -1.0 2,336,464 + RR42_RS31000 0.68 -1.1 2,336,465 - RR42_RS31000 0.68 +0.1
Or see this region's nucleotide sequence