Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS30770 and RR42_RS30775 are separated by 515 nucleotides RR42_RS30775 and RR42_RS30780 are separated by 251 nucleotides RR42_RS30780 and RR42_RS30785 are separated by 100 nucleotides
RR42_RS30770: RR42_RS30770 - hypothetical protein, at 2,281,699 to 2,282,478
_RS30770
RR42_RS30775: RR42_RS30775 - fatty acid hydroxylase, at 2,282,994 to 2,284,139
_RS30775
RR42_RS30780: RR42_RS30780 - tRNA-Leu, at 2,284,391 to 2,284,477
_RS30780
RR42_RS30785: RR42_RS30785 - cytosine deaminase, at 2,284,578 to 2,285,849
_RS30785
Position (kb)
2282
2283
2284
2285 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2282.056 kb on + strand, within RR42_RS30770 at 2282.056 kb on + strand, within RR42_RS30770 at 2282.083 kb on + strand, within RR42_RS30770 at 2282.083 kb on + strand, within RR42_RS30770 at 2282.084 kb on - strand, within RR42_RS30770 at 2282.247 kb on + strand, within RR42_RS30770 at 2282.248 kb on - strand, within RR42_RS30770 at 2282.280 kb on + strand, within RR42_RS30770 at 2282.280 kb on + strand, within RR42_RS30770 at 2282.281 kb on - strand, within RR42_RS30770 at 2282.281 kb on - strand, within RR42_RS30770 at 2282.655 kb on + strand at 2282.656 kb on - strand at 2282.761 kb on + strand at 2282.762 kb on - strand at 2282.785 kb on - strand at 2283.056 kb on + strand at 2283.126 kb on + strand, within RR42_RS30775 at 2283.145 kb on + strand, within RR42_RS30775 at 2283.146 kb on - strand, within RR42_RS30775 at 2283.156 kb on + strand, within RR42_RS30775 at 2283.156 kb on + strand, within RR42_RS30775 at 2283.157 kb on - strand, within RR42_RS30775 at 2283.157 kb on - strand, within RR42_RS30775 at 2283.343 kb on - strand, within RR42_RS30775 at 2283.343 kb on - strand, within RR42_RS30775 at 2283.423 kb on + strand, within RR42_RS30775 at 2283.519 kb on - strand, within RR42_RS30775 at 2283.639 kb on + strand, within RR42_RS30775 at 2283.639 kb on + strand, within RR42_RS30775 at 2283.640 kb on - strand, within RR42_RS30775 at 2283.640 kb on - strand, within RR42_RS30775 at 2283.658 kb on - strand, within RR42_RS30775 at 2283.825 kb on + strand, within RR42_RS30775 at 2283.897 kb on + strand, within RR42_RS30775 at 2283.966 kb on + strand, within RR42_RS30775 at 2283.966 kb on + strand, within RR42_RS30775 at 2283.967 kb on - strand, within RR42_RS30775 at 2283.993 kb on + strand, within RR42_RS30775 at 2283.993 kb on + strand, within RR42_RS30775 at 2284.042 kb on - strand at 2284.059 kb on + strand at 2284.060 kb on - strand at 2284.060 kb on - strand at 2284.060 kb on - strand at 2284.090 kb on + strand at 2284.090 kb on + strand at 2284.091 kb on - strand at 2284.091 kb on - strand at 2284.091 kb on - strand at 2284.091 kb on - strand at 2284.137 kb on + strand at 2284.137 kb on + strand at 2284.207 kb on + strand at 2284.272 kb on + strand at 2284.273 kb on - strand at 2284.365 kb on - strand at 2284.520 kb on - strand at 2284.766 kb on - strand, within RR42_RS30785 at 2284.865 kb on + strand, within RR42_RS30785 at 2284.866 kb on - strand, within RR42_RS30785 at 2285.000 kb on + strand, within RR42_RS30785 at 2285.000 kb on + strand, within RR42_RS30785 at 2285.000 kb on + strand, within RR42_RS30785 at 2285.001 kb on - strand, within RR42_RS30785 at 2285.001 kb on - strand, within RR42_RS30785 at 2285.001 kb on - strand, within RR42_RS30785 at 2285.001 kb on - strand, within RR42_RS30785 at 2285.001 kb on - strand, within RR42_RS30785 at 2285.021 kb on + strand, within RR42_RS30785 at 2285.021 kb on + strand, within RR42_RS30785 at 2285.021 kb on + strand, within RR42_RS30785 at 2285.022 kb on - strand, within RR42_RS30785 at 2285.022 kb on - strand, within RR42_RS30785 at 2285.092 kb on + strand, within RR42_RS30785 at 2285.092 kb on + strand, within RR42_RS30785 at 2285.093 kb on - strand, within RR42_RS30785 at 2285.093 kb on - strand, within RR42_RS30785 at 2285.093 kb on - strand, within RR42_RS30785 at 2285.093 kb on - strand, within RR42_RS30785 at 2285.095 kb on + strand, within RR42_RS30785 at 2285.095 kb on + strand, within RR42_RS30785 at 2285.095 kb on + strand, within RR42_RS30785 at 2285.095 kb on + strand, within RR42_RS30785 at 2285.095 kb on + strand, within RR42_RS30785 at 2285.095 kb on + strand, within RR42_RS30785 at 2285.095 kb on + strand, within RR42_RS30785 at 2285.096 kb on - strand, within RR42_RS30785
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 2,282,056 + RR42_RS30770 0.46 +0.8 2,282,056 + RR42_RS30770 0.46 +0.5 2,282,083 + RR42_RS30770 0.49 -1.0 2,282,083 + RR42_RS30770 0.49 -1.1 2,282,084 - RR42_RS30770 0.49 -0.4 2,282,247 + RR42_RS30770 0.70 -0.4 2,282,248 - RR42_RS30770 0.70 -0.8 2,282,280 + RR42_RS30770 0.74 -0.5 2,282,280 + RR42_RS30770 0.74 +0.4 2,282,281 - RR42_RS30770 0.75 -0.2 2,282,281 - RR42_RS30770 0.75 +0.0 2,282,655 + +0.0 2,282,656 - +0.6 2,282,761 + +0.0 2,282,762 - -0.1 2,282,785 - +0.2 2,283,056 + +1.0 2,283,126 + RR42_RS30775 0.12 -1.2 2,283,145 + RR42_RS30775 0.13 -1.4 2,283,146 - RR42_RS30775 0.13 +0.8 2,283,156 + RR42_RS30775 0.14 -0.1 2,283,156 + RR42_RS30775 0.14 +0.5 2,283,157 - RR42_RS30775 0.14 +0.3 2,283,157 - RR42_RS30775 0.14 -0.4 2,283,343 - RR42_RS30775 0.30 +0.6 2,283,343 - RR42_RS30775 0.30 -2.1 2,283,423 + RR42_RS30775 0.37 -1.3 2,283,519 - RR42_RS30775 0.46 +0.1 2,283,639 + RR42_RS30775 0.56 -0.4 2,283,639 + RR42_RS30775 0.56 -1.0 2,283,640 - RR42_RS30775 0.56 -0.4 2,283,640 - RR42_RS30775 0.56 +0.6 2,283,658 - RR42_RS30775 0.58 +0.4 2,283,825 + RR42_RS30775 0.73 -0.2 2,283,897 + RR42_RS30775 0.79 -0.4 2,283,966 + RR42_RS30775 0.85 +0.2 2,283,966 + RR42_RS30775 0.85 -0.6 2,283,967 - RR42_RS30775 0.85 +0.3 2,283,993 + RR42_RS30775 0.87 -0.8 2,283,993 + RR42_RS30775 0.87 +0.4 2,284,042 - +0.8 2,284,059 + -0.6 2,284,060 - -0.1 2,284,060 - -0.3 2,284,060 - -0.2 2,284,090 + -1.0 2,284,090 + +0.5 2,284,091 - +1.0 2,284,091 - -0.2 2,284,091 - -0.1 2,284,091 - -2.3 2,284,137 + +1.5 2,284,137 + -0.6 2,284,207 + +0.7 2,284,272 + -0.4 2,284,273 - -0.3 2,284,365 - -0.5 2,284,520 - -1.4 2,284,766 - RR42_RS30785 0.15 -0.8 2,284,865 + RR42_RS30785 0.23 +0.2 2,284,866 - RR42_RS30785 0.23 +0.7 2,285,000 + RR42_RS30785 0.33 +0.5 2,285,000 + RR42_RS30785 0.33 -1.7 2,285,000 + RR42_RS30785 0.33 +0.1 2,285,001 - RR42_RS30785 0.33 -0.1 2,285,001 - RR42_RS30785 0.33 +1.3 2,285,001 - RR42_RS30785 0.33 -1.7 2,285,001 - RR42_RS30785 0.33 +0.6 2,285,001 - RR42_RS30785 0.33 +0.0 2,285,021 + RR42_RS30785 0.35 +2.2 2,285,021 + RR42_RS30785 0.35 +1.2 2,285,021 + RR42_RS30785 0.35 -0.5 2,285,022 - RR42_RS30785 0.35 +0.5 2,285,022 - RR42_RS30785 0.35 -0.1 2,285,092 + RR42_RS30785 0.40 -0.1 2,285,092 + RR42_RS30785 0.40 +0.2 2,285,093 - RR42_RS30785 0.40 +0.1 2,285,093 - RR42_RS30785 0.40 +0.0 2,285,093 - RR42_RS30785 0.40 +0.2 2,285,093 - RR42_RS30785 0.40 +0.9 2,285,095 + RR42_RS30785 0.41 -0.1 2,285,095 + RR42_RS30785 0.41 -0.3 2,285,095 + RR42_RS30785 0.41 -0.5 2,285,095 + RR42_RS30785 0.41 -1.1 2,285,095 + RR42_RS30785 0.41 +0.6 2,285,095 + RR42_RS30785 0.41 +1.4 2,285,095 + RR42_RS30785 0.41 +0.3 2,285,096 - RR42_RS30785 0.41 +0.3
Or see this region's nucleotide sequence