Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27920 and RR42_RS27925 are separated by 47 nucleotides RR42_RS27925 and RR42_RS27930 are separated by 135 nucleotides
RR42_RS27920: RR42_RS27920 - hypothetical protein, at 1,627,592 to 1,628,158
_RS27920
RR42_RS27925: RR42_RS27925 - hypothetical protein, at 1,628,206 to 1,628,637
_RS27925
RR42_RS27930: RR42_RS27930 - amidohydrolase, at 1,628,773 to 1,629,936
_RS27930
Position (kb)
1628
1629
1630 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1627.863 kb on + strand, within RR42_RS27920 at 1627.864 kb on - strand, within RR42_RS27920 at 1627.864 kb on - strand, within RR42_RS27920 at 1627.925 kb on + strand, within RR42_RS27920 at 1627.925 kb on + strand, within RR42_RS27920 at 1627.926 kb on - strand, within RR42_RS27920 at 1628.005 kb on + strand, within RR42_RS27920 at 1628.006 kb on - strand, within RR42_RS27920 at 1628.009 kb on - strand, within RR42_RS27920 at 1628.009 kb on - strand, within RR42_RS27920 at 1628.013 kb on + strand, within RR42_RS27920 at 1628.013 kb on + strand, within RR42_RS27920 at 1628.013 kb on + strand, within RR42_RS27920 at 1628.013 kb on + strand, within RR42_RS27920 at 1628.014 kb on - strand, within RR42_RS27920 at 1628.020 kb on - strand, within RR42_RS27920 at 1628.151 kb on - strand at 1628.154 kb on - strand at 1628.154 kb on - strand at 1628.180 kb on + strand at 1628.231 kb on + strand at 1628.254 kb on + strand, within RR42_RS27925 at 1628.254 kb on + strand, within RR42_RS27925 at 1628.255 kb on - strand, within RR42_RS27925 at 1628.358 kb on + strand, within RR42_RS27925 at 1628.359 kb on - strand, within RR42_RS27925 at 1628.390 kb on + strand, within RR42_RS27925 at 1628.391 kb on - strand, within RR42_RS27925 at 1628.391 kb on - strand, within RR42_RS27925 at 1628.487 kb on + strand, within RR42_RS27925 at 1628.488 kb on - strand, within RR42_RS27925 at 1628.679 kb on + strand at 1628.786 kb on + strand at 1628.787 kb on - strand at 1628.787 kb on - strand at 1628.909 kb on + strand, within RR42_RS27930 at 1628.910 kb on - strand, within RR42_RS27930 at 1628.910 kb on - strand, within RR42_RS27930 at 1629.046 kb on - strand, within RR42_RS27930 at 1629.075 kb on - strand, within RR42_RS27930 at 1629.215 kb on + strand, within RR42_RS27930 at 1629.215 kb on + strand, within RR42_RS27930 at 1629.216 kb on - strand, within RR42_RS27930 at 1629.471 kb on - strand, within RR42_RS27930 at 1629.664 kb on + strand, within RR42_RS27930 at 1629.677 kb on + strand, within RR42_RS27930 at 1629.804 kb on - strand, within RR42_RS27930 at 1629.804 kb on - strand, within RR42_RS27930 at 1630.082 kb on - strand at 1630.082 kb on - strand at 1630.174 kb on + strand at 1630.174 kb on + strand at 1630.175 kb on - strand at 1630.175 kb on - strand at 1630.175 kb on - strand at 1630.175 kb on - strand at 1630.516 kb on + strand at 1630.516 kb on + strand at 1630.517 kb on - strand at 1630.517 kb on - strand at 1630.517 kb on - strand at 1630.517 kb on - strand at 1630.531 kb on + strand at 1630.531 kb on + strand at 1630.531 kb on + strand at 1630.531 kb on + strand at 1630.531 kb on + strand at 1630.532 kb on - strand at 1630.573 kb on - strand at 1630.661 kb on + strand at 1630.662 kb on - strand at 1630.663 kb on + strand at 1630.663 kb on + strand at 1630.663 kb on + strand at 1630.663 kb on + strand at 1630.663 kb on + strand at 1630.663 kb on + strand at 1630.663 kb on + strand at 1630.663 kb on + strand at 1630.664 kb on - strand at 1630.664 kb on - strand at 1630.664 kb on - strand at 1630.664 kb on - strand at 1630.664 kb on - strand at 1630.664 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 1,627,863 + RR42_RS27920 0.48 +0.3 1,627,864 - RR42_RS27920 0.48 +0.9 1,627,864 - RR42_RS27920 0.48 +0.3 1,627,925 + RR42_RS27920 0.59 +0.4 1,627,925 + RR42_RS27920 0.59 -0.7 1,627,926 - RR42_RS27920 0.59 +0.1 1,628,005 + RR42_RS27920 0.73 -0.0 1,628,006 - RR42_RS27920 0.73 -0.9 1,628,009 - RR42_RS27920 0.74 -0.4 1,628,009 - RR42_RS27920 0.74 -0.1 1,628,013 + RR42_RS27920 0.74 +1.2 1,628,013 + RR42_RS27920 0.74 +0.4 1,628,013 + RR42_RS27920 0.74 +1.3 1,628,013 + RR42_RS27920 0.74 +0.5 1,628,014 - RR42_RS27920 0.74 +0.6 1,628,020 - RR42_RS27920 0.75 +0.2 1,628,151 - +0.7 1,628,154 - -0.4 1,628,154 - +0.9 1,628,180 + -0.7 1,628,231 + +0.7 1,628,254 + RR42_RS27925 0.11 +0.7 1,628,254 + RR42_RS27925 0.11 -1.7 1,628,255 - RR42_RS27925 0.11 -1.8 1,628,358 + RR42_RS27925 0.35 +0.3 1,628,359 - RR42_RS27925 0.35 +0.1 1,628,390 + RR42_RS27925 0.43 -0.5 1,628,391 - RR42_RS27925 0.43 -0.7 1,628,391 - RR42_RS27925 0.43 +0.0 1,628,487 + RR42_RS27925 0.65 -0.5 1,628,488 - RR42_RS27925 0.65 +0.0 1,628,679 + +0.0 1,628,786 + -1.3 1,628,787 - -0.4 1,628,787 - +0.6 1,628,909 + RR42_RS27930 0.12 -0.2 1,628,910 - RR42_RS27930 0.12 +0.3 1,628,910 - RR42_RS27930 0.12 +0.6 1,629,046 - RR42_RS27930 0.23 +0.6 1,629,075 - RR42_RS27930 0.26 +0.4 1,629,215 + RR42_RS27930 0.38 -0.2 1,629,215 + RR42_RS27930 0.38 -0.1 1,629,216 - RR42_RS27930 0.38 +0.3 1,629,471 - RR42_RS27930 0.60 -0.0 1,629,664 + RR42_RS27930 0.77 -1.1 1,629,677 + RR42_RS27930 0.78 -0.4 1,629,804 - RR42_RS27930 0.89 -1.7 1,629,804 - RR42_RS27930 0.89 -0.2 1,630,082 - -0.3 1,630,082 - -1.0 1,630,174 + -1.7 1,630,174 + -1.6 1,630,175 - -1.3 1,630,175 - +1.0 1,630,175 - -1.1 1,630,175 - +0.2 1,630,516 + -0.4 1,630,516 + +0.7 1,630,517 - -0.5 1,630,517 - +1.9 1,630,517 - -0.6 1,630,517 - +0.2 1,630,531 + -0.0 1,630,531 + +1.0 1,630,531 + -1.6 1,630,531 + +0.5 1,630,531 + -1.1 1,630,532 - -1.1 1,630,573 - +1.7 1,630,661 + +0.2 1,630,662 - +0.6 1,630,663 + -0.4 1,630,663 + -0.5 1,630,663 + -1.0 1,630,663 + +0.7 1,630,663 + -0.6 1,630,663 + +1.6 1,630,663 + +0.6 1,630,663 + +1.2 1,630,664 - +0.3 1,630,664 - -0.7 1,630,664 - -1.1 1,630,664 - +0.1 1,630,664 - -0.4 1,630,664 - -0.3
Or see this region's nucleotide sequence