Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27525 and RR42_RS27530 are separated by 160 nucleotides RR42_RS27530 and RR42_RS27535 are separated by 71 nucleotides
RR42_RS27525: RR42_RS27525 - hypothetical protein, at 1,541,097 to 1,541,666
_RS27525
RR42_RS27530: RR42_RS27530 - hypothetical protein, at 1,541,827 to 1,542,315
_RS27530
RR42_RS27535: RR42_RS27535 - membrane protein, at 1,542,387 to 1,544,159
_RS27535
Position (kb)
1541
1542
1543 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1541.184 kb on + strand, within RR42_RS27525 at 1541.266 kb on + strand, within RR42_RS27525 at 1541.266 kb on + strand, within RR42_RS27525 at 1541.266 kb on + strand, within RR42_RS27525 at 1541.267 kb on - strand, within RR42_RS27525 at 1541.267 kb on - strand, within RR42_RS27525 at 1541.393 kb on + strand, within RR42_RS27525 at 1541.399 kb on + strand, within RR42_RS27525 at 1541.399 kb on + strand, within RR42_RS27525 at 1541.400 kb on - strand, within RR42_RS27525 at 1541.420 kb on - strand, within RR42_RS27525 at 1541.420 kb on - strand, within RR42_RS27525 at 1541.420 kb on - strand, within RR42_RS27525 at 1541.420 kb on - strand, within RR42_RS27525 at 1541.448 kb on - strand, within RR42_RS27525 at 1541.571 kb on + strand, within RR42_RS27525 at 1541.572 kb on - strand, within RR42_RS27525 at 1541.572 kb on - strand, within RR42_RS27525 at 1541.572 kb on - strand, within RR42_RS27525 at 1541.572 kb on - strand, within RR42_RS27525 at 1541.572 kb on - strand, within RR42_RS27525 at 1541.786 kb on + strand at 1541.787 kb on - strand at 1541.788 kb on + strand at 1541.789 kb on - strand at 1541.797 kb on - strand at 1541.802 kb on + strand at 1541.803 kb on - strand at 1541.971 kb on + strand, within RR42_RS27530 at 1542.121 kb on + strand, within RR42_RS27530 at 1542.121 kb on + strand, within RR42_RS27530 at 1542.121 kb on + strand at 1542.122 kb on - strand, within RR42_RS27530 at 1542.205 kb on + strand, within RR42_RS27530 at 1542.294 kb on + strand at 1542.346 kb on + strand at 1542.347 kb on - strand at 1542.347 kb on - strand at 1542.376 kb on + strand at 1542.376 kb on + strand at 1542.377 kb on - strand at 1542.377 kb on - strand at 1542.377 kb on - strand at 1542.377 kb on - strand at 1542.377 kb on - strand at 1542.844 kb on - strand, within RR42_RS27535 at 1542.844 kb on - strand, within RR42_RS27535 at 1543.251 kb on + strand, within RR42_RS27535 at 1543.312 kb on - strand, within RR42_RS27535
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 1,541,184 + RR42_RS27525 0.15 +0.1 1,541,266 + RR42_RS27525 0.30 +0.5 1,541,266 + RR42_RS27525 0.30 +0.0 1,541,266 + RR42_RS27525 0.30 -0.7 1,541,267 - RR42_RS27525 0.30 +0.2 1,541,267 - RR42_RS27525 0.30 +0.6 1,541,393 + RR42_RS27525 0.52 -0.2 1,541,399 + RR42_RS27525 0.53 -0.4 1,541,399 + RR42_RS27525 0.53 +0.0 1,541,400 - RR42_RS27525 0.53 -1.2 1,541,420 - RR42_RS27525 0.57 -0.2 1,541,420 - RR42_RS27525 0.57 -0.9 1,541,420 - RR42_RS27525 0.57 -0.6 1,541,420 - RR42_RS27525 0.57 -0.7 1,541,448 - RR42_RS27525 0.62 +0.5 1,541,571 + RR42_RS27525 0.83 -0.5 1,541,572 - RR42_RS27525 0.83 -0.8 1,541,572 - RR42_RS27525 0.83 +0.7 1,541,572 - RR42_RS27525 0.83 +0.2 1,541,572 - RR42_RS27525 0.83 +0.6 1,541,572 - RR42_RS27525 0.83 -0.2 1,541,786 + +0.1 1,541,787 - -0.5 1,541,788 + +0.1 1,541,789 - -0.0 1,541,797 - -0.2 1,541,802 + +1.2 1,541,803 - +0.1 1,541,971 + RR42_RS27530 0.29 +0.1 1,542,121 + RR42_RS27530 0.60 -0.6 1,542,121 + RR42_RS27530 0.60 -1.1 1,542,121 + -0.9 1,542,122 - RR42_RS27530 0.60 -0.7 1,542,205 + RR42_RS27530 0.77 -3.3 1,542,294 + +2.2 1,542,346 + -0.6 1,542,347 - -0.3 1,542,347 - -0.4 1,542,376 + -0.3 1,542,376 + -0.1 1,542,377 - +0.2 1,542,377 - +1.1 1,542,377 - +0.5 1,542,377 - -0.2 1,542,377 - +0.2 1,542,844 - RR42_RS27535 0.26 +0.8 1,542,844 - RR42_RS27535 0.26 +0.4 1,543,251 + RR42_RS27535 0.49 -1.7 1,543,312 - RR42_RS27535 0.52 -0.9
Or see this region's nucleotide sequence