Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27225 and RR42_RS27230 are separated by 459 nucleotides RR42_RS27230 and RR42_RS27235 are separated by 4 nucleotides
RR42_RS27225: RR42_RS27225 - tricarballylate utilization protein B, at 1,459,851 to 1,461,023
_RS27225
RR42_RS27230: RR42_RS27230 - DNA-binding protein, at 1,461,483 to 1,461,890
_RS27230
RR42_RS27235: RR42_RS27235 - acetyl-CoA acetyltransferase, at 1,461,895 to 1,463,061
_RS27235
Position (kb)
1461
1462 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1460.493 kb on + strand, within RR42_RS27225 at 1460.494 kb on - strand, within RR42_RS27225 at 1460.581 kb on - strand, within RR42_RS27225 at 1460.631 kb on + strand, within RR42_RS27225 at 1460.631 kb on + strand, within RR42_RS27225 at 1460.632 kb on - strand, within RR42_RS27225 at 1460.632 kb on - strand, within RR42_RS27225 at 1460.926 kb on - strand at 1460.949 kb on + strand at 1461.283 kb on + strand at 1461.634 kb on + strand, within RR42_RS27230 at 1461.634 kb on + strand, within RR42_RS27230 at 1461.758 kb on - strand, within RR42_RS27230 at 1461.877 kb on + strand at 1461.878 kb on - strand at 1461.878 kb on - strand at 1461.878 kb on - strand at 1461.893 kb on + strand at 1461.894 kb on - strand at 1461.894 kb on - strand at 1461.896 kb on + strand at 1462.106 kb on - strand, within RR42_RS27235 at 1462.232 kb on + strand, within RR42_RS27235 at 1462.233 kb on - strand, within RR42_RS27235 at 1462.233 kb on - strand, within RR42_RS27235 at 1462.486 kb on + strand, within RR42_RS27235 at 1462.556 kb on - strand, within RR42_RS27235 at 1462.602 kb on + strand, within RR42_RS27235 at 1462.602 kb on + strand, within RR42_RS27235 at 1462.602 kb on + strand, within RR42_RS27235 at 1462.603 kb on - strand, within RR42_RS27235 at 1462.603 kb on - strand, within RR42_RS27235 at 1462.806 kb on + strand, within RR42_RS27235 at 1462.807 kb on - strand, within RR42_RS27235 at 1462.809 kb on - strand, within RR42_RS27235 at 1462.809 kb on - strand, within RR42_RS27235 at 1462.809 kb on - strand, within RR42_RS27235 at 1462.809 kb on - strand, within RR42_RS27235 at 1462.862 kb on + strand, within RR42_RS27235 at 1462.863 kb on - strand, within RR42_RS27235 at 1462.872 kb on + strand, within RR42_RS27235
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 1,460,493 + RR42_RS27225 0.55 +0.2 1,460,494 - RR42_RS27225 0.55 +0.4 1,460,581 - RR42_RS27225 0.62 -0.5 1,460,631 + RR42_RS27225 0.66 -0.2 1,460,631 + RR42_RS27225 0.66 +0.9 1,460,632 - RR42_RS27225 0.67 +0.2 1,460,632 - RR42_RS27225 0.67 -0.1 1,460,926 - -0.0 1,460,949 + -1.3 1,461,283 + +0.4 1,461,634 + RR42_RS27230 0.37 -1.9 1,461,634 + RR42_RS27230 0.37 -1.3 1,461,758 - RR42_RS27230 0.67 -0.5 1,461,877 + -0.1 1,461,878 - +0.1 1,461,878 - -0.3 1,461,878 - +2.7 1,461,893 + +1.2 1,461,894 - +0.5 1,461,894 - +0.0 1,461,896 + -1.3 1,462,106 - RR42_RS27235 0.18 -0.4 1,462,232 + RR42_RS27235 0.29 +1.2 1,462,233 - RR42_RS27235 0.29 -1.9 1,462,233 - RR42_RS27235 0.29 -0.9 1,462,486 + RR42_RS27235 0.51 -0.3 1,462,556 - RR42_RS27235 0.57 -0.2 1,462,602 + RR42_RS27235 0.61 -0.4 1,462,602 + RR42_RS27235 0.61 +1.8 1,462,602 + RR42_RS27235 0.61 +0.2 1,462,603 - RR42_RS27235 0.61 +0.3 1,462,603 - RR42_RS27235 0.61 +0.5 1,462,806 + RR42_RS27235 0.78 -0.3 1,462,807 - RR42_RS27235 0.78 -0.9 1,462,809 - RR42_RS27235 0.78 -0.1 1,462,809 - RR42_RS27235 0.78 -0.4 1,462,809 - RR42_RS27235 0.78 +0.7 1,462,809 - RR42_RS27235 0.78 +0.6 1,462,862 + RR42_RS27235 0.83 -0.7 1,462,863 - RR42_RS27235 0.83 +0.8 1,462,872 + RR42_RS27235 0.84 +1.6
Or see this region's nucleotide sequence