Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27205 and RR42_RS27210 overlap by 4 nucleotides RR42_RS27210 and RR42_RS27215 are separated by 93 nucleotides RR42_RS27215 and RR42_RS27220 are separated by 184 nucleotides
RR42_RS27205: RR42_RS27205 - GlcNAc-PI de-N-acetylase, at 1,455,749 to 1,456,498
_RS27205
RR42_RS27210: RR42_RS27210 - hypothetical protein, at 1,456,495 to 1,457,208
_RS27210
RR42_RS27215: RR42_RS27215 - LysR family transcriptional regulator, at 1,457,302 to 1,458,252
_RS27215
RR42_RS27220: RR42_RS27220 - tricarballylate dehydrogenase, at 1,458,437 to 1,459,864
_RS27220
Position (kb)
1457
1458
1459 Strain fitness (log2 ratio)
-2
-1
0
1 at 1456.309 kb on + strand, within RR42_RS27205 at 1456.310 kb on - strand, within RR42_RS27205 at 1456.379 kb on + strand, within RR42_RS27205 at 1456.382 kb on + strand, within RR42_RS27205 at 1456.382 kb on + strand, within RR42_RS27205 at 1456.446 kb on - strand at 1456.457 kb on + strand at 1456.457 kb on + strand at 1456.458 kb on - strand at 1457.122 kb on + strand, within RR42_RS27210 at 1457.123 kb on - strand, within RR42_RS27210 at 1457.123 kb on - strand, within RR42_RS27210 at 1457.242 kb on - strand at 1457.309 kb on + strand at 1457.379 kb on + strand at 1457.664 kb on + strand, within RR42_RS27215 at 1457.665 kb on - strand, within RR42_RS27215 at 1457.665 kb on - strand, within RR42_RS27215 at 1458.147 kb on - strand, within RR42_RS27215 at 1458.311 kb on + strand at 1458.312 kb on - strand at 1458.876 kb on - strand, within RR42_RS27220 at 1458.876 kb on - strand, within RR42_RS27220 at 1458.876 kb on - strand, within RR42_RS27220 at 1459.181 kb on + strand, within RR42_RS27220 at 1459.181 kb on + strand, within RR42_RS27220 at 1459.181 kb on + strand, within RR42_RS27220
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 1,456,309 + RR42_RS27205 0.75 -0.1 1,456,310 - RR42_RS27205 0.75 +0.5 1,456,379 + RR42_RS27205 0.84 -0.4 1,456,382 + RR42_RS27205 0.84 -0.3 1,456,382 + RR42_RS27205 0.84 -0.8 1,456,446 - +0.1 1,456,457 + -0.2 1,456,457 + -2.6 1,456,458 - +0.9 1,457,122 + RR42_RS27210 0.88 +1.0 1,457,123 - RR42_RS27210 0.88 +0.3 1,457,123 - RR42_RS27210 0.88 -0.2 1,457,242 - -0.9 1,457,309 + -0.0 1,457,379 + -0.8 1,457,664 + RR42_RS27215 0.38 -1.1 1,457,665 - RR42_RS27215 0.38 -0.3 1,457,665 - RR42_RS27215 0.38 -0.1 1,458,147 - RR42_RS27215 0.89 -1.8 1,458,311 + +0.1 1,458,312 - -1.3 1,458,876 - RR42_RS27220 0.31 -1.6 1,458,876 - RR42_RS27220 0.31 -0.2 1,458,876 - RR42_RS27220 0.31 -0.7 1,459,181 + RR42_RS27220 0.52 -1.3 1,459,181 + RR42_RS27220 0.52 -0.8 1,459,181 + RR42_RS27220 0.52 -0.6
Or see this region's nucleotide sequence