Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS26925 and RR42_RS26930 are separated by 191 nucleotides RR42_RS26930 and RR42_RS26935 are separated by 132 nucleotides RR42_RS26935 and RR42_RS26940 are separated by 100 nucleotides
RR42_RS26925: RR42_RS26925 - membrane protein, at 1,396,695 to 1,396,847
_RS26925
RR42_RS26930: RR42_RS26930 - membrane protein, at 1,397,039 to 1,397,560
_RS26930
RR42_RS26935: RR42_RS26935 - farnesyl-diphosphate farnesyltransferase, at 1,397,693 to 1,398,745
_RS26935
RR42_RS26940: RR42_RS26940 - C4-dicarboxylate ABC transporter substrate-binding protein, at 1,398,846 to 1,400,159
_RS26940
Position (kb)
1397
1398
1399 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1396.825 kb on - strand, within RR42_RS26925 at 1396.825 kb on - strand, within RR42_RS26925 at 1396.917 kb on + strand at 1396.918 kb on - strand at 1396.981 kb on + strand at 1396.982 kb on - strand at 1396.982 kb on - strand at 1397.046 kb on - strand at 1397.193 kb on - strand, within RR42_RS26930 at 1397.225 kb on + strand, within RR42_RS26930 at 1397.226 kb on - strand, within RR42_RS26930 at 1397.324 kb on + strand, within RR42_RS26930 at 1397.324 kb on + strand, within RR42_RS26930 at 1397.324 kb on + strand, within RR42_RS26930 at 1397.324 kb on + strand, within RR42_RS26930 at 1397.324 kb on + strand, within RR42_RS26930 at 1397.325 kb on - strand, within RR42_RS26930 at 1397.399 kb on + strand, within RR42_RS26930 at 1397.403 kb on - strand, within RR42_RS26930 at 1397.453 kb on + strand, within RR42_RS26930 at 1397.454 kb on - strand, within RR42_RS26930 at 1397.454 kb on - strand, within RR42_RS26930 at 1397.477 kb on + strand, within RR42_RS26930 at 1397.478 kb on - strand, within RR42_RS26930 at 1397.478 kb on - strand, within RR42_RS26930 at 1397.694 kb on + strand at 1397.797 kb on - strand at 1397.946 kb on + strand, within RR42_RS26935 at 1398.085 kb on - strand, within RR42_RS26935 at 1398.217 kb on - strand, within RR42_RS26935 at 1398.261 kb on + strand, within RR42_RS26935 at 1398.262 kb on - strand, within RR42_RS26935 at 1398.262 kb on - strand, within RR42_RS26935 at 1398.627 kb on + strand, within RR42_RS26935 at 1398.681 kb on + strand at 1398.790 kb on + strand at 1398.878 kb on + strand at 1398.878 kb on + strand at 1398.878 kb on + strand at 1398.895 kb on + strand at 1398.896 kb on - strand at 1398.958 kb on - strand at 1399.045 kb on + strand, within RR42_RS26940 at 1399.201 kb on + strand, within RR42_RS26940 at 1399.201 kb on + strand, within RR42_RS26940 at 1399.201 kb on + strand, within RR42_RS26940 at 1399.201 kb on + strand, within RR42_RS26940 at 1399.202 kb on - strand, within RR42_RS26940 at 1399.202 kb on - strand, within RR42_RS26940 at 1399.202 kb on - strand, within RR42_RS26940 at 1399.204 kb on + strand, within RR42_RS26940 at 1399.205 kb on - strand, within RR42_RS26940 at 1399.205 kb on - strand, within RR42_RS26940 at 1399.450 kb on + strand, within RR42_RS26940 at 1399.466 kb on + strand, within RR42_RS26940 at 1399.466 kb on + strand, within RR42_RS26940 at 1399.466 kb on + strand at 1399.466 kb on + strand, within RR42_RS26940 at 1399.467 kb on - strand, within RR42_RS26940 at 1399.467 kb on - strand, within RR42_RS26940 at 1399.467 kb on - strand, within RR42_RS26940 at 1399.467 kb on - strand, within RR42_RS26940 at 1399.467 kb on - strand, within RR42_RS26940 at 1399.468 kb on + strand, within RR42_RS26940 at 1399.469 kb on - strand, within RR42_RS26940 at 1399.469 kb on - strand, within RR42_RS26940 at 1399.742 kb on + strand, within RR42_RS26940 at 1399.742 kb on + strand, within RR42_RS26940 at 1399.742 kb on + strand, within RR42_RS26940 at 1399.742 kb on + strand, within RR42_RS26940
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 1,396,825 - RR42_RS26925 0.85 +1.0 1,396,825 - RR42_RS26925 0.85 -2.8 1,396,917 + +1.4 1,396,918 - -1.1 1,396,981 + +0.9 1,396,982 - +0.1 1,396,982 - +0.7 1,397,046 - -0.4 1,397,193 - RR42_RS26930 0.30 +0.2 1,397,225 + RR42_RS26930 0.36 +0.1 1,397,226 - RR42_RS26930 0.36 -0.5 1,397,324 + RR42_RS26930 0.55 -1.0 1,397,324 + RR42_RS26930 0.55 +0.9 1,397,324 + RR42_RS26930 0.55 +0.5 1,397,324 + RR42_RS26930 0.55 +0.7 1,397,324 + RR42_RS26930 0.55 -0.8 1,397,325 - RR42_RS26930 0.55 +0.4 1,397,399 + RR42_RS26930 0.69 -0.0 1,397,403 - RR42_RS26930 0.70 -1.8 1,397,453 + RR42_RS26930 0.79 -2.1 1,397,454 - RR42_RS26930 0.80 -0.8 1,397,454 - RR42_RS26930 0.80 -0.5 1,397,477 + RR42_RS26930 0.84 +0.1 1,397,478 - RR42_RS26930 0.84 +0.3 1,397,478 - RR42_RS26930 0.84 -1.0 1,397,694 + -0.3 1,397,797 - -1.3 1,397,946 + RR42_RS26935 0.24 -2.7 1,398,085 - RR42_RS26935 0.37 -2.7 1,398,217 - RR42_RS26935 0.50 -2.3 1,398,261 + RR42_RS26935 0.54 -3.3 1,398,262 - RR42_RS26935 0.54 -3.0 1,398,262 - RR42_RS26935 0.54 -1.6 1,398,627 + RR42_RS26935 0.89 +0.7 1,398,681 + -2.4 1,398,790 + -0.7 1,398,878 + +3.3 1,398,878 + +0.7 1,398,878 + +0.7 1,398,895 + -0.5 1,398,896 - -0.1 1,398,958 - -0.2 1,399,045 + RR42_RS26940 0.15 -0.5 1,399,201 + RR42_RS26940 0.27 -0.5 1,399,201 + RR42_RS26940 0.27 +0.5 1,399,201 + RR42_RS26940 0.27 +0.7 1,399,201 + RR42_RS26940 0.27 -0.1 1,399,202 - RR42_RS26940 0.27 -0.0 1,399,202 - RR42_RS26940 0.27 +0.2 1,399,202 - RR42_RS26940 0.27 -0.9 1,399,204 + RR42_RS26940 0.27 -0.8 1,399,205 - RR42_RS26940 0.27 -0.1 1,399,205 - RR42_RS26940 0.27 +0.9 1,399,450 + RR42_RS26940 0.46 +0.7 1,399,466 + RR42_RS26940 0.47 +0.2 1,399,466 + RR42_RS26940 0.47 -0.1 1,399,466 + +0.2 1,399,466 + RR42_RS26940 0.47 -1.0 1,399,467 - RR42_RS26940 0.47 +0.2 1,399,467 - RR42_RS26940 0.47 -0.6 1,399,467 - RR42_RS26940 0.47 -0.8 1,399,467 - RR42_RS26940 0.47 +0.9 1,399,467 - RR42_RS26940 0.47 -0.6 1,399,468 + RR42_RS26940 0.47 +0.4 1,399,469 - RR42_RS26940 0.47 -0.4 1,399,469 - RR42_RS26940 0.47 +0.3 1,399,742 + RR42_RS26940 0.68 +0.3 1,399,742 + RR42_RS26940 0.68 +0.5 1,399,742 + RR42_RS26940 0.68 -1.1 1,399,742 + RR42_RS26940 0.68 +0.0
Or see this region's nucleotide sequence