Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS26760 and RR42_RS26765 are separated by 40 nucleotides RR42_RS26765 and RR42_RS26770 are separated by 58 nucleotides RR42_RS26770 and RR42_RS26775 are separated by 5 nucleotides
RR42_RS26760: RR42_RS26760 - hypothetical protein, at 1,357,748 to 1,358,161
_RS26760
RR42_RS26765: RR42_RS26765 - polyketide cyclase, at 1,358,202 to 1,358,738
_RS26765
RR42_RS26770: RR42_RS26770 - extradiol dioxygenase, at 1,358,797 to 1,359,213
_RS26770
RR42_RS26775: RR42_RS26775 - RNA polymerase subunit sigma-24, at 1,359,219 to 1,360,505
_RS26775
Position (kb)
1358
1359
1360 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1357.836 kb on - strand, within RR42_RS26760 at 1357.836 kb on - strand, within RR42_RS26760 at 1357.916 kb on + strand, within RR42_RS26760 at 1357.982 kb on + strand, within RR42_RS26760 at 1358.079 kb on + strand, within RR42_RS26760 at 1358.079 kb on + strand, within RR42_RS26760 at 1358.080 kb on - strand, within RR42_RS26760 at 1358.277 kb on - strand, within RR42_RS26765 at 1358.277 kb on - strand, within RR42_RS26765 at 1358.407 kb on - strand, within RR42_RS26765 at 1358.407 kb on - strand, within RR42_RS26765 at 1358.467 kb on + strand, within RR42_RS26765 at 1358.467 kb on + strand, within RR42_RS26765 at 1358.625 kb on + strand, within RR42_RS26765 at 1358.802 kb on + strand at 1358.803 kb on - strand at 1358.812 kb on + strand at 1358.813 kb on - strand at 1359.125 kb on - strand, within RR42_RS26770 at 1359.157 kb on - strand, within RR42_RS26770 at 1359.157 kb on - strand, within RR42_RS26770 at 1359.172 kb on + strand at 1359.172 kb on + strand at 1359.174 kb on + strand at 1359.174 kb on + strand at 1359.175 kb on - strand at 1359.517 kb on + strand, within RR42_RS26775 at 1359.518 kb on - strand, within RR42_RS26775 at 1359.822 kb on + strand, within RR42_RS26775 at 1359.822 kb on + strand, within RR42_RS26775 at 1359.822 kb on + strand, within RR42_RS26775 at 1360.209 kb on + strand, within RR42_RS26775
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 1,357,836 - RR42_RS26760 0.21 -0.8 1,357,836 - RR42_RS26760 0.21 -0.2 1,357,916 + RR42_RS26760 0.41 -0.0 1,357,982 + RR42_RS26760 0.57 -1.7 1,358,079 + RR42_RS26760 0.80 +0.1 1,358,079 + RR42_RS26760 0.80 -0.4 1,358,080 - RR42_RS26760 0.80 +1.0 1,358,277 - RR42_RS26765 0.14 -0.0 1,358,277 - RR42_RS26765 0.14 -1.5 1,358,407 - RR42_RS26765 0.38 +1.0 1,358,407 - RR42_RS26765 0.38 +0.4 1,358,467 + RR42_RS26765 0.49 -0.0 1,358,467 + RR42_RS26765 0.49 +0.1 1,358,625 + RR42_RS26765 0.79 +0.8 1,358,802 + -1.1 1,358,803 - +0.0 1,358,812 + +2.4 1,358,813 - -0.3 1,359,125 - RR42_RS26770 0.79 -0.8 1,359,157 - RR42_RS26770 0.86 -1.9 1,359,157 - RR42_RS26770 0.86 -1.4 1,359,172 + +0.3 1,359,172 + -0.2 1,359,174 + -0.3 1,359,174 + -1.4 1,359,175 - -0.2 1,359,517 + RR42_RS26775 0.23 -0.8 1,359,518 - RR42_RS26775 0.23 -0.2 1,359,822 + RR42_RS26775 0.47 -0.2 1,359,822 + RR42_RS26775 0.47 -0.1 1,359,822 + RR42_RS26775 0.47 -0.1 1,360,209 + RR42_RS26775 0.77 +0.2
Or see this region's nucleotide sequence