Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS26550 and RR42_RS26555 are separated by 235 nucleotides RR42_RS26555 and RR42_RS26560 are separated by 269 nucleotides RR42_RS26560 and RR42_RS26565 are separated by 166 nucleotides
RR42_RS26550: RR42_RS26550 - gluconolactonase, at 1,312,159 to 1,313,103
_RS26550
RR42_RS26555: RR42_RS26555 - tRNA-Met, at 1,313,339 to 1,313,414
_RS26555
RR42_RS26560: RR42_RS26560 - hypothetical protein, at 1,313,684 to 1,313,923
_RS26560
RR42_RS26565: RR42_RS26565 - 5'-3' exonuclease, at 1,314,090 to 1,314,896
_RS26565
Position (kb)
1313
1314 Strain fitness (log2 ratio)
-1
0
1 at 1312.806 kb on + strand, within RR42_RS26550 at 1312.806 kb on + strand, within RR42_RS26550 at 1312.806 kb on + strand, within RR42_RS26550 at 1312.814 kb on - strand, within RR42_RS26550 at 1312.888 kb on + strand, within RR42_RS26550 at 1312.888 kb on + strand, within RR42_RS26550 at 1312.888 kb on + strand, within RR42_RS26550 at 1312.889 kb on - strand, within RR42_RS26550 at 1312.889 kb on - strand, within RR42_RS26550 at 1313.321 kb on + strand at 1313.321 kb on + strand at 1313.378 kb on + strand, within RR42_RS26555 at 1313.425 kb on + strand at 1313.448 kb on + strand at 1313.464 kb on + strand at 1313.465 kb on - strand at 1313.582 kb on + strand at 1313.582 kb on + strand at 1313.850 kb on + strand, within RR42_RS26560 at 1313.850 kb on + strand, within RR42_RS26560 at 1313.851 kb on - strand, within RR42_RS26560 at 1313.851 kb on - strand, within RR42_RS26560 at 1313.851 kb on - strand, within RR42_RS26560 at 1313.891 kb on + strand, within RR42_RS26560 at 1313.892 kb on - strand, within RR42_RS26560 at 1313.933 kb on + strand at 1313.934 kb on - strand at 1313.934 kb on - strand at 1313.934 kb on - strand at 1314.041 kb on - strand at 1314.103 kb on + strand at 1314.104 kb on - strand at 1314.104 kb on - strand at 1314.104 kb on - strand at 1314.104 kb on - strand at 1314.104 kb on - strand at 1314.295 kb on - strand, within RR42_RS26565 at 1314.295 kb on - strand, within RR42_RS26565 at 1314.411 kb on + strand, within RR42_RS26565 at 1314.444 kb on + strand, within RR42_RS26565 at 1314.445 kb on - strand, within RR42_RS26565 at 1314.478 kb on + strand, within RR42_RS26565 at 1314.589 kb on - strand, within RR42_RS26565 at 1314.589 kb on - strand, within RR42_RS26565 at 1314.634 kb on - strand, within RR42_RS26565 at 1314.653 kb on + strand, within RR42_RS26565 at 1314.665 kb on + strand, within RR42_RS26565 at 1314.666 kb on - strand, within RR42_RS26565 at 1314.921 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 1,312,806 + RR42_RS26550 0.68 -0.5 1,312,806 + RR42_RS26550 0.68 +0.8 1,312,806 + RR42_RS26550 0.68 +0.6 1,312,814 - RR42_RS26550 0.69 +0.5 1,312,888 + RR42_RS26550 0.77 +1.0 1,312,888 + RR42_RS26550 0.77 +1.5 1,312,888 + RR42_RS26550 0.77 -1.0 1,312,889 - RR42_RS26550 0.77 +0.9 1,312,889 - RR42_RS26550 0.77 +0.6 1,313,321 + +0.3 1,313,321 + -0.1 1,313,378 + RR42_RS26555 0.51 +0.8 1,313,425 + -0.2 1,313,448 + -0.7 1,313,464 + -0.3 1,313,465 - -1.0 1,313,582 + -1.0 1,313,582 + +0.3 1,313,850 + RR42_RS26560 0.69 -1.1 1,313,850 + RR42_RS26560 0.69 +1.4 1,313,851 - RR42_RS26560 0.70 +0.1 1,313,851 - RR42_RS26560 0.70 -0.2 1,313,851 - RR42_RS26560 0.70 -1.2 1,313,891 + RR42_RS26560 0.86 +0.8 1,313,892 - RR42_RS26560 0.87 +0.1 1,313,933 + +0.8 1,313,934 - -0.5 1,313,934 - +0.8 1,313,934 - +0.1 1,314,041 - -0.9 1,314,103 + -0.2 1,314,104 - +0.4 1,314,104 - -1.0 1,314,104 - +0.7 1,314,104 - +1.4 1,314,104 - +0.3 1,314,295 - RR42_RS26565 0.25 +0.5 1,314,295 - RR42_RS26565 0.25 -0.7 1,314,411 + RR42_RS26565 0.40 -1.1 1,314,444 + RR42_RS26565 0.44 -0.6 1,314,445 - RR42_RS26565 0.44 -0.2 1,314,478 + RR42_RS26565 0.48 -0.3 1,314,589 - RR42_RS26565 0.62 -0.3 1,314,589 - RR42_RS26565 0.62 -0.5 1,314,634 - RR42_RS26565 0.67 -0.1 1,314,653 + RR42_RS26565 0.70 -0.2 1,314,665 + RR42_RS26565 0.71 -1.3 1,314,666 - RR42_RS26565 0.71 -0.8 1,314,921 - +0.6
Or see this region's nucleotide sequence