Experiment: R2A with A22 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS26540 and RR42_RS26545 are separated by 89 nucleotides RR42_RS26545 and RR42_RS26550 are separated by 3 nucleotides
RR42_RS26540: RR42_RS26540 - 3-phosphoglycerate dehydrogenase, at 1,310,147 to 1,311,082
_RS26540
RR42_RS26545: RR42_RS26545 - ABC transporter substrate-binding protein, at 1,311,172 to 1,312,155
_RS26545
RR42_RS26550: RR42_RS26550 - gluconolactonase, at 1,312,159 to 1,313,103
_RS26550
Position (kb)
1311
1312
1313 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1310.309 kb on + strand, within RR42_RS26540 at 1311.011 kb on + strand at 1311.012 kb on - strand at 1311.012 kb on - strand at 1311.012 kb on - strand at 1311.012 kb on - strand at 1311.012 kb on - strand at 1311.019 kb on + strand at 1311.020 kb on - strand at 1311.020 kb on - strand at 1311.043 kb on + strand at 1311.412 kb on - strand, within RR42_RS26545 at 1311.472 kb on + strand, within RR42_RS26545 at 1311.472 kb on + strand, within RR42_RS26545 at 1311.514 kb on + strand, within RR42_RS26545 at 1311.514 kb on + strand, within RR42_RS26545 at 1311.515 kb on - strand, within RR42_RS26545 at 1311.589 kb on + strand, within RR42_RS26545 at 1311.646 kb on + strand, within RR42_RS26545 at 1311.646 kb on + strand, within RR42_RS26545 at 1311.647 kb on - strand, within RR42_RS26545 at 1311.647 kb on - strand, within RR42_RS26545 at 1311.647 kb on - strand, within RR42_RS26545 at 1312.153 kb on + strand at 1312.153 kb on + strand at 1312.154 kb on - strand at 1312.154 kb on - strand at 1312.154 kb on - strand at 1312.324 kb on + strand, within RR42_RS26550 at 1312.324 kb on + strand, within RR42_RS26550 at 1312.325 kb on - strand, within RR42_RS26550 at 1312.325 kb on - strand, within RR42_RS26550 at 1312.325 kb on - strand, within RR42_RS26550 at 1312.325 kb on - strand, within RR42_RS26550 at 1312.325 kb on - strand, within RR42_RS26550 at 1312.359 kb on + strand, within RR42_RS26550 at 1312.360 kb on - strand, within RR42_RS26550 at 1312.507 kb on + strand, within RR42_RS26550 at 1312.579 kb on + strand, within RR42_RS26550 at 1312.579 kb on + strand, within RR42_RS26550 at 1312.579 kb on + strand, within RR42_RS26550 at 1312.579 kb on + strand, within RR42_RS26550 at 1312.580 kb on - strand, within RR42_RS26550 at 1312.580 kb on - strand, within RR42_RS26550 at 1312.618 kb on - strand, within RR42_RS26550 at 1312.618 kb on - strand, within RR42_RS26550 at 1312.630 kb on + strand, within RR42_RS26550 at 1312.806 kb on + strand, within RR42_RS26550 at 1312.806 kb on + strand, within RR42_RS26550 at 1312.806 kb on + strand, within RR42_RS26550 at 1312.814 kb on - strand, within RR42_RS26550 at 1312.888 kb on + strand, within RR42_RS26550 at 1312.888 kb on + strand, within RR42_RS26550 at 1312.888 kb on + strand, within RR42_RS26550 at 1312.889 kb on - strand, within RR42_RS26550 at 1312.889 kb on - strand, within RR42_RS26550
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.01 mg/ml remove 1,310,309 + RR42_RS26540 0.17 +1.2 1,311,011 + +0.1 1,311,012 - +0.2 1,311,012 - -0.2 1,311,012 - -0.5 1,311,012 - -0.5 1,311,012 - +0.8 1,311,019 + +0.1 1,311,020 - -0.3 1,311,020 - -2.0 1,311,043 + -0.2 1,311,412 - RR42_RS26545 0.24 +1.0 1,311,472 + RR42_RS26545 0.30 +0.3 1,311,472 + RR42_RS26545 0.30 +1.1 1,311,514 + RR42_RS26545 0.35 -0.4 1,311,514 + RR42_RS26545 0.35 -0.3 1,311,515 - RR42_RS26545 0.35 +0.5 1,311,589 + RR42_RS26545 0.42 -0.1 1,311,646 + RR42_RS26545 0.48 -0.5 1,311,646 + RR42_RS26545 0.48 +0.6 1,311,647 - RR42_RS26545 0.48 -1.2 1,311,647 - RR42_RS26545 0.48 +0.2 1,311,647 - RR42_RS26545 0.48 +2.8 1,312,153 + +0.0 1,312,153 + -0.3 1,312,154 - +0.4 1,312,154 - +0.2 1,312,154 - +0.2 1,312,324 + RR42_RS26550 0.17 -0.2 1,312,324 + RR42_RS26550 0.17 +0.4 1,312,325 - RR42_RS26550 0.18 -0.9 1,312,325 - RR42_RS26550 0.18 +0.8 1,312,325 - RR42_RS26550 0.18 -0.0 1,312,325 - RR42_RS26550 0.18 +0.2 1,312,325 - RR42_RS26550 0.18 -0.2 1,312,359 + RR42_RS26550 0.21 +1.3 1,312,360 - RR42_RS26550 0.21 -0.1 1,312,507 + RR42_RS26550 0.37 -0.7 1,312,579 + RR42_RS26550 0.44 +0.3 1,312,579 + RR42_RS26550 0.44 -2.0 1,312,579 + RR42_RS26550 0.44 -0.7 1,312,579 + RR42_RS26550 0.44 -0.5 1,312,580 - RR42_RS26550 0.45 +0.9 1,312,580 - RR42_RS26550 0.45 +2.6 1,312,618 - RR42_RS26550 0.49 -0.3 1,312,618 - RR42_RS26550 0.49 -0.0 1,312,630 + RR42_RS26550 0.50 +0.7 1,312,806 + RR42_RS26550 0.68 -0.5 1,312,806 + RR42_RS26550 0.68 +0.8 1,312,806 + RR42_RS26550 0.68 +0.6 1,312,814 - RR42_RS26550 0.69 +0.5 1,312,888 + RR42_RS26550 0.77 +1.0 1,312,888 + RR42_RS26550 0.77 +1.5 1,312,888 + RR42_RS26550 0.77 -1.0 1,312,889 - RR42_RS26550 0.77 +0.9 1,312,889 - RR42_RS26550 0.77 +0.6
Or see this region's nucleotide sequence